Visualize the Distribution and Dynamics of Mitochondrial DNA (mtDNA) Nucleoids with Multiple Labeling Strategies.

Di, X; Qin, J; Sun, Y; Su, QP

Visualize the Distribution and Dynamics of Mitochondrial DNA (mtDNA) Nucleoids with Multiple Labeling Strategies.

Di, X Qin, J Sun, Y Su, QP

Permalink

Publisher:: Springer Nature
Publication Type:: Chapter
Citation:: Methods in Molecular Biology, 2023, 2615, pp. 79-88
Issue Date:: 2023

Closed Access

	Filename	Description	Size
	978-1-0716-2922-2_6.pdf		384.3 kB	Adobe PDF	View/Open

Copyright Clearance Process

Recently Added
In Progress
Closed Access

This item is closed access and not available.

Full metadata record

Field	Value	Language
dc.contributor.author	Di, X
dc.contributor.author	Qin, J
dc.contributor.author	Sun, Y
dc.contributor.author	Su, QP
dc.date.accessioned	2023-10-18T23:47:22Z
dc.date.available	2023-10-18T23:47:22Z
dc.date.issued	2023
dc.identifier.citation	Methods in Molecular Biology, 2023, 2615, pp. 79-88
dc.identifier.isbn	9781071629215
dc.identifier.uri	http://hdl.handle.net/10453/172804
dc.description.abstract	Mitochondrial DNA (mtDNA) encodes a variety of rRNAs, tRNAs, and respiratory chain complex proteins. The integrity of mtDNA supports the mitochondrial functions and plays an essential role in numerous physiological and pathological processes. Mutations in mtDNA cause metabolic diseases and aging. The mtDNA within the human cells are packaged into hundreds of nucleoids within the mitochondrial matrix. Knowledge of how the nucleoids are dynamically distributed and organized within mitochondria is key to understanding mtDNA structure and functions. Therefore, visualizing the distribution and dynamics of mtDNA within mitochondria is a powerful approach to gain insights into the regulation of mtDNA replication and transcription. In this chapter, we describe the methods of observing mtDNA and its replication with fluorescence microscopy in both fixed and live cells using different labeling strategies.
dc.format	Print
dc.language	en
dc.publisher	Springer Nature
dc.relation	http://purl.org/au-research/grants/nhmrc/1177374
dc.relation.ispartof	Methods in Molecular Biology
dc.relation.ispartofseries	Methods in Molecular Biology
dc.relation.isbasedon	10.1007/978-1-0716-2922-2_6
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0399 Other Chemical Sciences, 0601 Biochemistry and Cell Biology
dc.subject.classification	Developmental Biology
dc.subject.classification	3101 Biochemistry and cell biology
dc.subject.classification	3404 Medicinal and biomolecular chemistry
dc.subject.mesh	Humans
dc.subject.mesh	DNA, Mitochondrial
dc.subject.mesh	Mitochondrial Proteins
dc.subject.mesh	Mitochondria
dc.subject.mesh	DNA Replication
dc.subject.mesh	Mitochondrial Membranes
dc.subject.mesh	Mitochondrial Dynamics
dc.subject.mesh	Mitochondria
dc.subject.mesh	Humans
dc.subject.mesh	Mitochondrial Proteins
dc.subject.mesh	DNA, Mitochondrial
dc.subject.mesh	DNA Replication
dc.subject.mesh	Mitochondrial Membranes
dc.subject.mesh	Mitochondrial Dynamics
dc.subject.mesh	Humans
dc.subject.mesh	DNA, Mitochondrial
dc.subject.mesh	Mitochondrial Proteins
dc.subject.mesh	Mitochondria
dc.subject.mesh	DNA Replication
dc.subject.mesh	Mitochondrial Membranes
dc.subject.mesh	Mitochondrial Dynamics
dc.title	Visualize the Distribution and Dynamics of Mitochondrial DNA (mtDNA) Nucleoids with Multiple Labeling Strategies.
dc.type	Chapter
utslib.citation.volume	2615
utslib.for	0399 Other Chemical Sciences
utslib.for	0601 Biochemistry and Cell Biology
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
utslib.copyright.status	closed_access	*
dc.date.updated	2023-10-18T23:47:21Z
pubs.publication-status	Published
pubs.volume	2615

Abstract:

Mitochondrial DNA (mtDNA) encodes a variety of rRNAs, tRNAs, and respiratory chain complex proteins. The integrity of mtDNA supports the mitochondrial functions and plays an essential role in numerous physiological and pathological processes. Mutations in mtDNA cause metabolic diseases and aging. The mtDNA within the human cells are packaged into hundreds of nucleoids within the mitochondrial matrix. Knowledge of how the nucleoids are dynamically distributed and organized within mitochondria is key to understanding mtDNA structure and functions. Therefore, visualizing the distribution and dynamics of mtDNA within mitochondria is a powerful approach to gain insights into the regulation of mtDNA replication and transcription. In this chapter, we describe the methods of observing mtDNA and its replication with fluorescence microscopy in both fixed and live cells using different labeling strategies.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/172804