Zerumbone liquid crystalline nanoparticles protect against oxidative stress, inflammation and senescence induced by cigarette smoke extract in vitro

Paudel, KR; Clarence, DD; Panth, N; Manandhar, B; De Rubis, G; Devkota, HP; Gupta, G; Zacconi, FC; Williams, KA; Pont, LG; Singh, SK; Warkiani, ME; Adams, J; MacLoughlin, R; Oliver, BG; Chellappan, DK; Hansbro, PM; Dua, K

Zerumbone liquid crystalline nanoparticles protect against oxidative stress, inflammation and senescence induced by cigarette smoke extract in vitro

Paudel, KR Clarence, DD Panth, N Manandhar, B De Rubis, G Devkota, HP Gupta, G Zacconi, FC Williams, KA Pont, LG Singh, SK Warkiani, ME Adams, J MacLoughlin, R Oliver, BG Chellappan, DK Hansbro, PM Dua, K

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Publisher:: Springer Science and Business Media LLC
Publication Type:: Journal Article
Citation:: Naunyn-Schmiedeberg's Archives of Pharmacology

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Field	Value	Language
dc.contributor.author	Paudel, KR
dc.contributor.author	Clarence, DD
dc.contributor.author	Panth, N
dc.contributor.author	Manandhar, B
dc.contributor.author	De Rubis, G
dc.contributor.author	Devkota, HP
dc.contributor.author	Gupta, G
dc.contributor.author	Zacconi, FC
dc.contributor.author	Williams, KA
dc.contributor.author	Pont, LG
dc.contributor.author	Singh, SK
dc.contributor.author	Warkiani, ME
dc.contributor.author	Adams, J
dc.contributor.author	MacLoughlin, R
dc.contributor.author	Oliver, BG
dc.contributor.author	Chellappan, DK
dc.contributor.author	Hansbro, PM
dc.contributor.author	Dua, K https://orcid.org/0000-0002-7507-1159
dc.date.accessioned	2023-10-19T11:36:23Z
dc.date.available	2023-10-19T11:36:23Z
dc.identifier.citation	Naunyn-Schmiedeberg's Archives of Pharmacology
dc.identifier.issn	0028-1298
dc.identifier.issn	1432-1912
dc.identifier.uri	http://hdl.handle.net/10453/172811
dc.description.abstract	<jats:title>Abstract</jats:title><jats:p>The purpose of this study was to evaluate the potential of zerumbone-loaded liquid crystalline nanoparticles (ZER-LCNs) in the protection of broncho-epithelial cells and alveolar macrophages against oxidative stress, inflammation and senescence induced by cigarette smoke extract <jats:italic>in vitro</jats:italic>. The effect of the treatment of ZER-LCNs on <jats:italic>in vitro</jats:italic> cell models of cigarette smoke extract (CSE)-treated mouse RAW264.7 and human BCi-NS1.1 basal epithelial cell lines was evaluated for their anti-inflammatory, antioxidant and anti-senescence activities using colorimetric and fluorescence-based assays, fluorescence imaging, RT-qPCR and proteome profiler kit. The ZER-LCNs successfully reduced the expression of pro-inflammatory markers including <jats:italic>Il-6</jats:italic>, <jats:italic>Il-1β</jats:italic> and <jats:italic>Tnf-α</jats:italic>, as well as the production of nitric oxide in RAW 264.7 cells. Additionally, ZER-LCNs successfully inhibited oxidative stress through reduction of reactive oxygen species (ROS) levels and regulation of genes, namely <jats:italic>GPX2</jats:italic> and <jats:italic>GCLC</jats:italic> in BCi-NS1.1 cells. Anti-senescence activity of ZER-LCNs was also observed in BCi-NS1.1 cells, with significant reductions in the expression of <jats:italic>SIRT1</jats:italic>, <jats:italic>CDKN1A</jats:italic> and <jats:italic>CDKN2A</jats:italic>. This study demonstrates strong <jats:italic>in vitro</jats:italic> anti-inflammatory, antioxidative and anti-senescence activities of ZER-LCNs paving the path for this formulation to be translated into a promising therapeutic agent for chronic respiratory inflammatory conditions including COPD and asthma.</jats:p>
dc.language	en
dc.publisher	Springer Science and Business Media LLC
dc.relation.ispartof	Naunyn-Schmiedeberg's Archives of Pharmacology
dc.relation.isbasedon	10.1007/s00210-023-02760-7
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	1115 Pharmacology and Pharmaceutical Sciences, 1116 Medical Physiology
dc.subject.classification	Pharmacology & Pharmacy
dc.subject.classification	3214 Pharmacology and pharmaceutical sciences
dc.title	Zerumbone liquid crystalline nanoparticles protect against oxidative stress, inflammation and senescence induced by cigarette smoke extract in vitro
dc.type	Journal Article
utslib.for	1115 Pharmacology and Pharmaceutical Sciences
utslib.for	1116 Medical Physiology
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Health
utslib.copyright.status	open_access	*
dc.date.updated	2023-10-19T11:36:20Z
pubs.publication-status	Published online

Abstract:

AbstractThe purpose of this study was to evaluate the potential of zerumbone-loaded liquid crystalline nanoparticles (ZER-LCNs) in the protection of broncho-epithelial cells and alveolar macrophages against oxidative stress, inflammation and senescence induced by cigarette smoke extract in vitro. The effect of the treatment of ZER-LCNs on in vitro cell models of cigarette smoke extract (CSE)-treated mouse RAW264.7 and human BCi-NS1.1 basal epithelial cell lines was evaluated for their anti-inflammatory, antioxidant and anti-senescence activities using colorimetric and fluorescence-based assays, fluorescence imaging, RT-qPCR and proteome profiler kit. The ZER-LCNs successfully reduced the expression of pro-inflammatory markers including Il-6, Il-1β and Tnf-α, as well as the production of nitric oxide in RAW 264.7 cells. Additionally, ZER-LCNs successfully inhibited oxidative stress through reduction of reactive oxygen species (ROS) levels and regulation of genes, namely GPX2 and GCLC in BCi-NS1.1 cells. Anti-senescence activity of ZER-LCNs was also observed in BCi-NS1.1 cells, with significant reductions in the expression of SIRT1, CDKN1A and CDKN2A. This study demonstrates strong in vitro anti-inflammatory, antioxidative and anti-senescence activities of ZER-LCNs paving the path for this formulation to be translated into a promising therapeutic agent for chronic respiratory inflammatory conditions including COPD and asthma.

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http://hdl.handle.net/10453/172811