Development of an In Situ Printing System With Human Platelet Lysate-Based Bio-Adhesive to Treat Corneal Perforations.
You, J
Frazer, H
Sayyar, S
Chen, Z
Liu, X
Taylor, A
Filippi, B
Beirne, S
Wise, I
Petsoglou, C
Hodge, C
Wallace, G
Sutton, G
- Publisher:
- Association for Research in Vision and Ophthalmology (ARVO)
- Publication Type:
- Journal Article
- Citation:
- Transl Vis Sci Technol, 2022, 11, (6), pp. 26
- Issue Date:
- 2022-06-01
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Full metadata record
Field | Value | Language |
---|---|---|
dc.contributor.author | You, J | |
dc.contributor.author | Frazer, H | |
dc.contributor.author | Sayyar, S | |
dc.contributor.author | Chen, Z | |
dc.contributor.author | Liu, X | |
dc.contributor.author | Taylor, A | |
dc.contributor.author | Filippi, B | |
dc.contributor.author | Beirne, S | |
dc.contributor.author | Wise, I | |
dc.contributor.author | Petsoglou, C | |
dc.contributor.author |
Hodge, C https://orcid.org/0000-0002-0516-6486 |
|
dc.contributor.author | Wallace, G | |
dc.contributor.author | Sutton, G | |
dc.date.accessioned | 2024-01-04T03:09:52Z | |
dc.date.available | 2024-01-04T03:09:52Z | |
dc.date.issued | 2022-06-01 | |
dc.identifier.citation | Transl Vis Sci Technol, 2022, 11, (6), pp. 26 | |
dc.identifier.issn | 2164-2591 | |
dc.identifier.issn | 2164-2591 | |
dc.identifier.uri | http://hdl.handle.net/10453/173987 | |
dc.description.abstract | PURPOSE: Corneal perforation is a clinical emergency that can result in blindness. Currently corneal perforations are treated either by cyanoacrylate glue which is toxic to corneal cells, or by using commercial fibrin glue for small perforations. Both methods use manual delivery which lead to uncontrolled application of the glues to the corneal surface. Therefore, there is a need to develop a safe and effective alternative to artificial adhesives. METHODS: Previously, our group developed a transparent human platelet lysate (hPL)-based biomaterial that accelerated corneal epithelial cells healing in vitro. This biomaterial was further characterized in this study using rheometry and adhesive test, and a two-component delivery system was developed for its application. An animal trial (5 New Zealand white rabbits) to compare impact of the biomaterial and cyanoacrylate glue (control group) on a 2 mm perforation was conducted to evaluate safety and efficacy. RESULTS: The hPL-based biomaterial showed higher adhesiveness compared to commercial fibrin glue. Treatment rabbits had lower pain scores and faster recovery, despite generating similar scar-forming structure compared to controls. No secondary corneal ulcer was generated in rabbits treated with the bio-adhesive. CONCLUSIONS: This study reports an in situ printing system capable of delivering a hPL-based, transparent bio-adhesive and successfully treating small corneal perforations. The bio-adhesive-treated rabbits recovered faster and required no additional analgesia. TRANSLATIONAL RELEVANCE: The developed in situ hPL bio-adhesives treatment represents a new format of treating corneal perforation that is easy to use, allows for accurate application, and can be a potentially effective and pain relief treatment. | |
dc.format | ||
dc.language | eng | |
dc.publisher | Association for Research in Vision and Ophthalmology (ARVO) | |
dc.relation.ispartof | Transl Vis Sci Technol | |
dc.relation.isbasedon | 10.1167/tvst.11.6.26 | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject | 0903 Biomedical Engineering, 1113 Opthalmology and Optometry | |
dc.subject.classification | 3212 Ophthalmology and optometry | |
dc.subject.mesh | Adhesives | |
dc.subject.mesh | Animals | |
dc.subject.mesh | Biocompatible Materials | |
dc.subject.mesh | Corneal Perforation | |
dc.subject.mesh | Cyanoacrylates | |
dc.subject.mesh | Fibrin Tissue Adhesive | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Pain | |
dc.subject.mesh | Printing, Three-Dimensional | |
dc.subject.mesh | Rabbits | |
dc.subject.mesh | Tissue Adhesives | |
dc.subject.mesh | Animals | |
dc.subject.mesh | Rabbits | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Pain | |
dc.subject.mesh | Cyanoacrylates | |
dc.subject.mesh | Fibrin Tissue Adhesive | |
dc.subject.mesh | Biocompatible Materials | |
dc.subject.mesh | Tissue Adhesives | |
dc.subject.mesh | Adhesives | |
dc.subject.mesh | Corneal Perforation | |
dc.subject.mesh | Printing, Three-Dimensional | |
dc.subject.mesh | Adhesives | |
dc.subject.mesh | Animals | |
dc.subject.mesh | Biocompatible Materials | |
dc.subject.mesh | Corneal Perforation | |
dc.subject.mesh | Cyanoacrylates | |
dc.subject.mesh | Fibrin Tissue Adhesive | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Pain | |
dc.subject.mesh | Printing, Three-Dimensional | |
dc.subject.mesh | Rabbits | |
dc.subject.mesh | Tissue Adhesives | |
dc.title | Development of an In Situ Printing System With Human Platelet Lysate-Based Bio-Adhesive to Treat Corneal Perforations. | |
dc.type | Journal Article | |
utslib.citation.volume | 11 | |
utslib.location.activity | United States | |
utslib.for | 0903 Biomedical Engineering | |
utslib.for | 1113 Opthalmology and Optometry | |
pubs.organisational-group | /University of Technology Sydney | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Health | |
utslib.copyright.status | open_access | * |
dc.date.updated | 2024-01-04T03:09:47Z | |
pubs.issue | 6 | |
pubs.publication-status | Published | |
pubs.volume | 11 | |
utslib.citation.issue | 6 |
Abstract:
PURPOSE: Corneal perforation is a clinical emergency that can result in blindness. Currently corneal perforations are treated either by cyanoacrylate glue which is toxic to corneal cells, or by using commercial fibrin glue for small perforations. Both methods use manual delivery which lead to uncontrolled application of the glues to the corneal surface. Therefore, there is a need to develop a safe and effective alternative to artificial adhesives. METHODS: Previously, our group developed a transparent human platelet lysate (hPL)-based biomaterial that accelerated corneal epithelial cells healing in vitro. This biomaterial was further characterized in this study using rheometry and adhesive test, and a two-component delivery system was developed for its application. An animal trial (5 New Zealand white rabbits) to compare impact of the biomaterial and cyanoacrylate glue (control group) on a 2 mm perforation was conducted to evaluate safety and efficacy. RESULTS: The hPL-based biomaterial showed higher adhesiveness compared to commercial fibrin glue. Treatment rabbits had lower pain scores and faster recovery, despite generating similar scar-forming structure compared to controls. No secondary corneal ulcer was generated in rabbits treated with the bio-adhesive. CONCLUSIONS: This study reports an in situ printing system capable of delivering a hPL-based, transparent bio-adhesive and successfully treating small corneal perforations. The bio-adhesive-treated rabbits recovered faster and required no additional analgesia. TRANSLATIONAL RELEVANCE: The developed in situ hPL bio-adhesives treatment represents a new format of treating corneal perforation that is easy to use, allows for accurate application, and can be a potentially effective and pain relief treatment.
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