Optical Nonlinearity Enabled Super-Resolved Multiplexing Microscopy.

Ding, L; Chen, C; Shan, X; Liu, B; Wang, D; Du, Z; Zhao, G; Su, QP; Yang, Y; Halkon, B; Tran, TT; Liao, J; Aharonovich, I; Zhang, M; Cheng, F; Fu, L; Xu, X; Wang, F

Optical Nonlinearity Enabled Super-Resolved Multiplexing Microscopy.

Ding, L Chen, C

Shan, X Liu, B Wang, D Du, Z Zhao, G Su, QP Yang, Y

Halkon, B

Tran, TT Liao, J

Aharonovich, I

Zhang, M Cheng, F Fu, L Xu, X

Wang, F

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Publisher:: WILEY-V C H VERLAG GMBH
Publication Type:: Journal Article
Citation:: Adv Mater, 2023, pp. e2308844
Issue Date:: 2023-11-16

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Field	Value	Language
dc.contributor.author	Ding, L
dc.contributor.author	Chen, C https://orcid.org/0000-0003-4620-7771
dc.contributor.author	Shan, X
dc.contributor.author	Liu, B
dc.contributor.author	Wang, D
dc.contributor.author	Du, Z
dc.contributor.author	Zhao, G
dc.contributor.author	Su, QP
dc.contributor.author	Yang, Y https://orcid.org/0000-0001-7439-2156
dc.contributor.author	Halkon, B https://orcid.org/0000-0001-5459-2329
dc.contributor.author	Tran, TT
dc.contributor.author	Liao, J https://orcid.org/0000-0003-0616-4762
dc.contributor.author	Aharonovich, I https://orcid.org/0000-0003-4304-3935
dc.contributor.author	Zhang, M
dc.contributor.author	Cheng, F
dc.contributor.author	Fu, L
dc.contributor.author	Xu, X https://orcid.org/0000-0002-2598-3766
dc.contributor.author	Wang, F
dc.date.accessioned	2024-01-09T05:43:35Z
dc.date.available	2024-01-09T05:43:35Z
dc.date.issued	2023-11-16
dc.identifier.citation	Adv Mater, 2023, pp. e2308844
dc.identifier.issn	0935-9648
dc.identifier.issn	1521-4095
dc.identifier.uri	http://hdl.handle.net/10453/174154
dc.description.abstract	Optical multiplexing for nanoscale object recognition is of great significance within the intricate domains of biology, medicine, anti-counterfeiting, and microscopic imaging. Traditionally, the multiplexing dimensions of nanoscopy are limited to emission intensity, color, lifetime, and polarization. Here, a novel dimension, optical nonlinearity, is proposed for super-resolved multiplexing microscopy. This optical nonlinearity is attributable to the energy transitions between multiple energy levels of the doped lanthanide ions in upconversion nanoparticles (UCNPs), resulting in unique optical fingerprints for UCNPs with different compositions. A vortex beam is applied to transport the optical nonlinearity onto the imaging point-spread function (PSF), creating a robust super-resolved multiplexing imaging strategy for differentiating UCNPs with distinctive optical nonlinearities. The composition information of the nanoparticles can be retrieved with variations of the corresponding PSF in the obtained image. Four channels multiplexing super-resolved imaging with a single scanning, applying emission color and nonlinearity of two orthogonal imaging dimensions with a spatial resolution higher than 150 nm (1/6.5λ), are demonstrated. This work provides a new and orthogonal dimension - optical nonlinearity - to existing multiplexing dimensions, which shows great potential in bioimaging, anti-counterfeiting, microarray assays, deep tissue multiplexing detection, and high-density data storage.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	WILEY-V C H VERLAG GMBH
dc.relation.ispartof	Adv Mater
dc.relation.isbasedon	10.1002/adma.202308844
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	02 Physical Sciences, 03 Chemical Sciences, 09 Engineering
dc.subject.classification	Nanoscience & Nanotechnology
dc.subject.classification	34 Chemical sciences
dc.subject.classification	40 Engineering
dc.subject.classification	51 Physical sciences
dc.title	Optical Nonlinearity Enabled Super-Resolved Multiplexing Microscopy.
dc.type	Journal Article
utslib.location.activity	Germany
utslib.for	02 Physical Sciences
utslib.for	03 Chemical Sciences
utslib.for	09 Engineering
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Mechanical and Mechatronic Engineering
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Electrical and Data Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
utslib.copyright.status	open_access	*
dc.date.updated	2024-01-09T05:43:34Z
pubs.publication-status	Published online

Abstract:

Optical multiplexing for nanoscale object recognition is of great significance within the intricate domains of biology, medicine, anti-counterfeiting, and microscopic imaging. Traditionally, the multiplexing dimensions of nanoscopy are limited to emission intensity, color, lifetime, and polarization. Here, a novel dimension, optical nonlinearity, is proposed for super-resolved multiplexing microscopy. This optical nonlinearity is attributable to the energy transitions between multiple energy levels of the doped lanthanide ions in upconversion nanoparticles (UCNPs), resulting in unique optical fingerprints for UCNPs with different compositions. A vortex beam is applied to transport the optical nonlinearity onto the imaging point-spread function (PSF), creating a robust super-resolved multiplexing imaging strategy for differentiating UCNPs with distinctive optical nonlinearities. The composition information of the nanoparticles can be retrieved with variations of the corresponding PSF in the obtained image. Four channels multiplexing super-resolved imaging with a single scanning, applying emission color and nonlinearity of two orthogonal imaging dimensions with a spatial resolution higher than 150 nm (1/6.5λ), are demonstrated. This work provides a new and orthogonal dimension - optical nonlinearity - to existing multiplexing dimensions, which shows great potential in bioimaging, anti-counterfeiting, microarray assays, deep tissue multiplexing detection, and high-density data storage.

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http://hdl.handle.net/10453/174154