Quantitative Point of Care Tests for Timely Diagnosis of Early‐Onset Preeclampsia with High Sensitivity and Specificity

Ghorbanpour, SM; Wen, S; Kaitu'u‐Lino, TJ; Hannan, NJ; Jin, D; McClements, L

Quantitative Point of Care Tests for Timely Diagnosis of Early‐Onset Preeclampsia with High Sensitivity and Specificity

Ghorbanpour, SM Wen, S Kaitu'u‐Lino, TJ Hannan, NJ Jin, D

McClements, L

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Publisher:: Wiley
Publication Type:: Journal Article
Citation:: Angewandte Chemie, 2023, 135, (26)
Issue Date:: 2023-06-26

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Field	Value	Language
dc.contributor.author	Ghorbanpour, SM
dc.contributor.author	Wen, S
dc.contributor.author	Kaitu'u‐Lino, TJ
dc.contributor.author	Hannan, NJ
dc.contributor.author	Jin, D https://orcid.org/0000-0003-1046-2666
dc.contributor.author	McClements, L https://orcid.org/0000-0002-4911-1014
dc.date.accessioned	2024-02-12T06:00:45Z
dc.date.available	2024-02-12T06:00:45Z
dc.date.issued	2023-06-26
dc.identifier.citation	Angewandte Chemie, 2023, 135, (26)
dc.identifier.issn	0044-8249
dc.identifier.issn	1521-3757
dc.identifier.uri	http://hdl.handle.net/10453/175583
dc.description.abstract	<jats:title>Abstract</jats:title><jats:p>Preeclampsia is a heterogeneous and multiorgan cardiovascular disorder of pregnancy. Here, we report the development of a novel strip‐based lateral flow assay (LFA) using lanthanide‐doped upconversion nanoparticles conjugated to antibodies targeting two different biomarkers for detection of preeclampsia. We first measured circulating plasma FKBPL and CD44 protein concentrations from individuals with early‐onset preeclampsia (EOPE), using ELISA. We confirmed that the CD44/FKBPL ratio is reduced in EOPE with a good diagnostic potential. Using our rapid LFA prototypes, we achieved an improved lower limit of detection: 10 pg ml<jats:sup>−1</jats:sup> for FKBPL and 15 pg ml<jats:sup>−1</jats:sup> for CD44, which is more than one order lower than the standard ELISA method. Using clinical samples, a cut‐off value of 1.24 for CD44/FKBPL ratio provided positive predictive value of 100 % and the negative predictive value of 91 %. Our LFA shows promise as a rapid and highly sensitive point‐of‐care test for preeclampsia.</jats:p>
dc.language	en
dc.publisher	Wiley
dc.relation	http://purl.org/au-research/grants/arc/DE220100846
dc.relation	http://purl.org/au-research/grants/arc/IH150100028
dc.relation	http://purl.org/au-research/grants/arc/FL210100180
dc.relation.ispartof	Angewandte Chemie
dc.relation.isbasedon	10.1002/ange.202301193
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	03 Chemical Sciences
dc.subject.classification	Organic Chemistry
dc.subject.classification	34 Chemical sciences
dc.title	Quantitative Point of Care Tests for Timely Diagnosis of Early‐Onset Preeclampsia with High Sensitivity and Specificity
dc.type	Journal Article
utslib.citation.volume	135
utslib.for	03 Chemical Sciences
pubs.organisational-group	University of Technology Sydney
pubs.organisational-group	University of Technology Sydney/Faculty of Science
pubs.organisational-group	University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
utslib.copyright.status	open_access	*
dc.date.updated	2024-02-12T06:00:44Z
pubs.issue	26
pubs.publication-status	Published
pubs.volume	135
utslib.citation.issue	26

Abstract:

AbstractPreeclampsia is a heterogeneous and multiorgan cardiovascular disorder of pregnancy. Here, we report the development of a novel strip‐based lateral flow assay (LFA) using lanthanide‐doped upconversion nanoparticles conjugated to antibodies targeting two different biomarkers for detection of preeclampsia. We first measured circulating plasma FKBPL and CD44 protein concentrations from individuals with early‐onset preeclampsia (EOPE), using ELISA. We confirmed that the CD44/FKBPL ratio is reduced in EOPE with a good diagnostic potential. Using our rapid LFA prototypes, we achieved an improved lower limit of detection: 10 pg ml−1 for FKBPL and 15 pg ml−1 for CD44, which is more than one order lower than the standard ELISA method. Using clinical samples, a cut‐off value of 1.24 for CD44/FKBPL ratio provided positive predictive value of 100 % and the negative predictive value of 91 %. Our LFA shows promise as a rapid and highly sensitive point‐of‐care test for preeclampsia.

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http://hdl.handle.net/10453/175583