Advances in TEER measurements of biological barriers in microphysiological systems.

Nazari, H; Shrestha, J; Naei, VY; Bazaz, SR; Sabbagh, M; Thiery, JP; Warkiani, ME

Advances in TEER measurements of biological barriers in microphysiological systems.

Nazari, H Shrestha, J

Naei, VY Bazaz, SR Sabbagh, M Thiery, JP Warkiani, ME

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Publisher:: ELSEVIER ADVANCED TECHNOLOGY
Publication Type:: Journal Article
Citation:: Biosens Bioelectron, 2023, 234, pp. 115355
Issue Date:: 2023-08-15

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Field	Value	Language
dc.contributor.author	Nazari, H
dc.contributor.author	Shrestha, J https://orcid.org/0000-0003-3692-6676
dc.contributor.author	Naei, VY
dc.contributor.author	Bazaz, SR
dc.contributor.author	Sabbagh, M
dc.contributor.author	Thiery, JP
dc.contributor.author	Warkiani, ME
dc.date.accessioned	2024-03-08T02:20:16Z
dc.date.available	2023-04-25
dc.date.available	2024-03-08T02:20:16Z
dc.date.issued	2023-08-15
dc.identifier.citation	Biosens Bioelectron, 2023, 234, pp. 115355
dc.identifier.issn	0956-5663
dc.identifier.issn	1873-4235
dc.identifier.uri	http://hdl.handle.net/10453/176342
dc.description.abstract	Biological barriers are multicellular structures that precisely regulate the transport of ions, biomolecules, drugs, cells, and other organisms. Transendothelial/epithelial electrical resistance (TEER) is a label-free method for predicting the properties of biological barriers. Understanding the mechanisms that control TEER significantly enhances our knowledge of the physiopathology of different diseases and aids in the development of new drugs. Measuring TEER values within microphysiological systems called organ-on-a-chip devices that simulate the microenvironment, architecture, and physiology of biological barriers in the body provides valuable insight into the behavior of barriers in response to different drugs and pathogens. These integrated systems should increase the accuracy, reproducibility, sensitivity, resolution, high throughput, speed, cost-effectiveness, and reliable predictability of TEER measurements. Implementing advanced micro and nanoscale manufacturing techniques, surface modification methods, biomaterials, biosensors, electronics, and stem cell biology is necessary for integrating TEER measuring systems with organ-on-chip technology. This review focuses on the applications, advantages, and future perspectives of integrating organ-on-a-chip technology with TEER measurement methods for studying biological barriers. After briefly reviewing the role of TEER in the physiology and pathology of barriers, standard techniques for measuring TEER, including Ohm's law and impedance spectroscopy, and commercially available devices are described. Furthermore, advances in TEER measurement are discussed in multiple barrier-on-a-chip system models representing different organs. Finally, we outline future trends in implementing advanced technologies to design and fabricate nanostructured electrodes, complicated microfluidic chips, and membranes for more advanced and accurate TEER measurements.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	ELSEVIER ADVANCED TECHNOLOGY
dc.relation.ispartof	Biosens Bioelectron
dc.relation.isbasedon	10.1016/j.bios.2023.115355
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0301 Analytical Chemistry, 0903 Biomedical Engineering, 1007 Nanotechnology
dc.subject.classification	Bioinformatics
dc.subject.classification	3401 Analytical chemistry
dc.subject.classification	4003 Biomedical engineering
dc.subject.classification	4018 Nanotechnology
dc.subject.mesh	Microphysiological Systems
dc.subject.mesh	Electric Impedance
dc.subject.mesh	Reproducibility of Results
dc.subject.mesh	Biosensing Techniques
dc.subject.mesh	Microfluidics
dc.subject.mesh	Lab-On-A-Chip Devices
dc.subject.mesh	Reproducibility of Results
dc.subject.mesh	Biosensing Techniques
dc.subject.mesh	Microfluidics
dc.subject.mesh	Electric Impedance
dc.subject.mesh	Lab-On-A-Chip Devices
dc.subject.mesh	Microphysiological Systems
dc.subject.mesh	Microphysiological Systems
dc.subject.mesh	Electric Impedance
dc.subject.mesh	Reproducibility of Results
dc.subject.mesh	Biosensing Techniques
dc.subject.mesh	Microfluidics
dc.subject.mesh	Lab-On-A-Chip Devices
dc.title	Advances in TEER measurements of biological barriers in microphysiological systems.
dc.type	Journal Article
utslib.citation.volume	234
utslib.location.activity	England
utslib.for	0301 Analytical Chemistry
utslib.for	0903 Biomedical Engineering
utslib.for	1007 Nanotechnology
pubs.organisational-group	University of Technology Sydney
pubs.organisational-group	University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	University of Technology Sydney/Faculty of Science
pubs.organisational-group	University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
utslib.copyright.status	closed_access	*
dc.date.updated	2024-03-08T02:20:12Z
pubs.publication-status	Published
pubs.volume	234

Abstract:

Biological barriers are multicellular structures that precisely regulate the transport of ions, biomolecules, drugs, cells, and other organisms. Transendothelial/epithelial electrical resistance (TEER) is a label-free method for predicting the properties of biological barriers. Understanding the mechanisms that control TEER significantly enhances our knowledge of the physiopathology of different diseases and aids in the development of new drugs. Measuring TEER values within microphysiological systems called organ-on-a-chip devices that simulate the microenvironment, architecture, and physiology of biological barriers in the body provides valuable insight into the behavior of barriers in response to different drugs and pathogens. These integrated systems should increase the accuracy, reproducibility, sensitivity, resolution, high throughput, speed, cost-effectiveness, and reliable predictability of TEER measurements. Implementing advanced micro and nanoscale manufacturing techniques, surface modification methods, biomaterials, biosensors, electronics, and stem cell biology is necessary for integrating TEER measuring systems with organ-on-chip technology. This review focuses on the applications, advantages, and future perspectives of integrating organ-on-a-chip technology with TEER measurement methods for studying biological barriers. After briefly reviewing the role of TEER in the physiology and pathology of barriers, standard techniques for measuring TEER, including Ohm's law and impedance spectroscopy, and commercially available devices are described. Furthermore, advances in TEER measurement are discussed in multiple barrier-on-a-chip system models representing different organs. Finally, we outline future trends in implementing advanced technologies to design and fabricate nanostructured electrodes, complicated microfluidic chips, and membranes for more advanced and accurate TEER measurements.

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http://hdl.handle.net/10453/176342