Scaling up stem cell production: harnessing the potential of microfluidic devices.

Ding, L; Oh, S; Shrestha, J; Lam, A; Wang, Y; Radfar, P; Warkiani, ME

Scaling up stem cell production: harnessing the potential of microfluidic devices.

Ding, L Oh, S Shrestha, J

Lam, A Wang, Y Radfar, P Warkiani, ME

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Publisher:: PERGAMON-ELSEVIER SCIENCE LTD
Publication Type:: Journal Article
Citation:: Biotechnol Adv, 2023, 69, pp. 108271
Issue Date:: 2023-12

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Field	Value	Language
dc.contributor.author	Ding, L
dc.contributor.author	Oh, S
dc.contributor.author	Shrestha, J https://orcid.org/0000-0003-3692-6676
dc.contributor.author	Lam, A
dc.contributor.author	Wang, Y
dc.contributor.author	Radfar, P
dc.contributor.author	Warkiani, ME
dc.date.accessioned	2024-03-08T03:39:42Z
dc.date.available	2023-10-13
dc.date.available	2024-03-08T03:39:42Z
dc.date.issued	2023-12
dc.identifier.citation	Biotechnol Adv, 2023, 69, pp. 108271
dc.identifier.issn	0734-9750
dc.identifier.issn	1873-1899
dc.identifier.uri	http://hdl.handle.net/10453/176348
dc.description.abstract	Stem cells are specialised cells characterised by their unique ability to both self-renew and transform into a wide array of specialised cell types. The widespread interest in stem cells for regenerative medicine and cultivated meat has led to a significant demand for these cells in both research and practical applications. Despite the growing need for stem cell manufacturing, the industry faces significant obstacles, including high costs for equipment and maintenance, complicated operation, and low product quality and yield. Microfluidic technology presents a promising solution to the abovementioned challenges. As an innovative approach for manipulating liquids and cells within microchannels, microfluidics offers a plethora of advantages at an industrial scale. These benefits encompass low setup costs, ease of operation and multiplexing, minimal energy consumption, and the added advantage of being labour-free. This review presents a thorough examination of the prominent microfluidic technologies employed in stem cell research and explores their promising applications in the burgeoning stem cell industry. It thoroughly examines how microfluidics can enhance cell harvesting from tissue samples, facilitate mixing and cryopreservation, streamline microcarrier production, and efficiently conduct cell separation, purification, washing, and final cell formulation post-culture.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	PERGAMON-ELSEVIER SCIENCE LTD
dc.relation.ispartof	Biotechnol Adv
dc.relation.isbasedon	10.1016/j.biotechadv.2023.108271
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	06 Biological Sciences, 09 Engineering, 10 Technology
dc.subject.classification	Biotechnology
dc.subject.classification	31 Biological sciences
dc.subject.classification	40 Engineering
dc.subject.mesh	Microfluidics
dc.subject.mesh	Regenerative Medicine
dc.subject.mesh	Stem Cells
dc.subject.mesh	Cell Culture Techniques
dc.subject.mesh	Lab-On-A-Chip Devices
dc.subject.mesh	Stem Cells
dc.subject.mesh	Cell Culture Techniques
dc.subject.mesh	Microfluidics
dc.subject.mesh	Regenerative Medicine
dc.subject.mesh	Lab-On-A-Chip Devices
dc.subject.mesh	Microfluidics
dc.subject.mesh	Regenerative Medicine
dc.subject.mesh	Stem Cells
dc.subject.mesh	Cell Culture Techniques
dc.subject.mesh	Lab-On-A-Chip Devices
dc.title	Scaling up stem cell production: harnessing the potential of microfluidic devices.
dc.type	Journal Article
utslib.citation.volume	69
utslib.location.activity	England
utslib.for	06 Biological Sciences
utslib.for	09 Engineering
utslib.for	10 Technology
pubs.organisational-group	University of Technology Sydney
pubs.organisational-group	University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	University of Technology Sydney/Faculty of Science
pubs.organisational-group	University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
utslib.copyright.status	closed_access	*
dc.date.updated	2024-03-08T03:39:35Z
pubs.publication-status	Published
pubs.volume	69

Abstract:

Stem cells are specialised cells characterised by their unique ability to both self-renew and transform into a wide array of specialised cell types. The widespread interest in stem cells for regenerative medicine and cultivated meat has led to a significant demand for these cells in both research and practical applications. Despite the growing need for stem cell manufacturing, the industry faces significant obstacles, including high costs for equipment and maintenance, complicated operation, and low product quality and yield. Microfluidic technology presents a promising solution to the abovementioned challenges. As an innovative approach for manipulating liquids and cells within microchannels, microfluidics offers a plethora of advantages at an industrial scale. These benefits encompass low setup costs, ease of operation and multiplexing, minimal energy consumption, and the added advantage of being labour-free. This review presents a thorough examination of the prominent microfluidic technologies employed in stem cell research and explores their promising applications in the burgeoning stem cell industry. It thoroughly examines how microfluidics can enhance cell harvesting from tissue samples, facilitate mixing and cryopreservation, streamline microcarrier production, and efficiently conduct cell separation, purification, washing, and final cell formulation post-culture.

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http://hdl.handle.net/10453/176348