(‐)‐Epigallocatechin‐3‐gallate induced apoptosis by dissociation of c‐FLIP/Ku70 complex in gastric cancer cells
- Publisher:
- Wiley
- Publication Type:
- Journal Article
- Citation:
- Journal of Cellular and Molecular Medicine, 2023, 27, (17), pp. 2572-2582
- Issue Date:
- 2023-09
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Anti cancer properties of epigallocatechin 3 gallate EGCG are mediated via apoptosis induction as well as inhibition of cell proliferation and histone deacetylase Accumulation of stabilized cellular FLICE inhibitory protein c FLIP Ku70 complex in the cytoplasm inhibits apoptosis through interruption of extrinsic apoptosis pathway In this study we evaluated the anti cancer role of EGCG in gastric cancer GC cells through dissociation of c FLIP Ku70 complex MKN 45 cells were treated with EGCG or its antagonist MG149 for 24 h Apoptosis was evaluated by flow cytometry and quantitative RT PCR Protein expression of c FLIP and Ku70 was analysed using western blot and immunofluorescence Dissociation of c FLIP Ku70 complex as well as Ku70 translocation were studied by sub cellular fractionation and co immunoprecipitation EGCG induced apoptosis in MKN 45 cells with substantial up regulation of P53 and P21 down regulation of c Myc and Cyclin D1 as well as cell cycle arrest in S and G2 M check points Moreover EGCG treatment suppressed the expression of c FLIP and Ku70 decreased their interaction while increasing the Ku70 nuclear content By dissociating the c FLIP Ku70 complex EGCG could be an alternative component to the conventional HDAC inhibitors in order to induce apoptosis in GC cells Thus its combination with other cancer therapy protocols could result in a better therapeutic outcome
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