Irradiation-Induced Dysbiosis: The Compounding Effect of High-Fat Diet on Metabolic and Immune Functions in Mice.

Clifford, BK; Amorim, NML; Kaakoush, NO; Boysen, L; Tedla, N; Goldstein, D; Hardeman, EC; Simar, D

Irradiation-Induced Dysbiosis: The Compounding Effect of High-Fat Diet on Metabolic and Immune Functions in Mice.

Clifford, BK Amorim, NML Kaakoush, NO Boysen, L Tedla, N Goldstein, D Hardeman, EC Simar, D

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Publisher:: MDPI
Publication Type:: Journal Article
Citation:: Int J Mol Sci, 2023, 24, (6), pp. 5631
Issue Date:: 2023-03-15

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Field	Value	Language
dc.contributor.author	Clifford, BK
dc.contributor.author	Amorim, NML
dc.contributor.author	Kaakoush, NO
dc.contributor.author	Boysen, L
dc.contributor.author	Tedla, N
dc.contributor.author	Goldstein, D
dc.contributor.author	Hardeman, EC
dc.contributor.author	Simar, D
dc.date.accessioned	2024-03-27T02:17:05Z
dc.date.available	2023-03-13
dc.date.available	2024-03-27T02:17:05Z
dc.date.issued	2023-03-15
dc.identifier.citation	Int J Mol Sci, 2023, 24, (6), pp. 5631
dc.identifier.issn	1422-0067
dc.identifier.issn	1422-0067
dc.identifier.uri	http://hdl.handle.net/10453/177227
dc.description.abstract	The negative impact of irradiation or diet on the metabolic and immune profiles of cancer survivors have been previously demonstrated. The gut microbiota plays a critical role in regulating these functions and is highly sensitive to cancer therapies. The aim of this study was to investigate the effect of irradiation and diet on the gut microbiota and metabolic or immune functions. We exposed C57Bl/6J mice to a single dose of 6 Gy radiation and after 5 weeks, fed them a chow or high-fat diet (HFD) for 12 weeks. We characterised their faecal microbiota, metabolic (whole body and adipose tissue) functions, and systemic (multiplex cytokine, chemokine assay, and immune cell profiling) and adipose tissue inflammatory profiles (immune cell profiling). At the end of the study, we observed a compounding effect of irradiation and diet on the metabolic and immune profiles of adipose tissue, with exposed mice fed a HFD displaying a greater inflammatory signature and impaired metabolism. Mice fed a HFD also showed altered microbiota, irrespective of irradiation status. An altered diet may exacerbate the detrimental effects of irradiation on both the metabolic and inflammatory profiles. This could have implications for the diagnosis and prevention of metabolic complications in cancer survivors exposed to radiation.
dc.format	Electronic
dc.language	eng
dc.publisher	MDPI
dc.relation.ispartof	Int J Mol Sci
dc.relation.isbasedon	10.3390/ijms24065631
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	0399 Other Chemical Sciences, 0604 Genetics, 0699 Other Biological Sciences
dc.subject.classification	Chemical Physics
dc.subject.classification	3101 Biochemistry and cell biology
dc.subject.classification	3107 Microbiology
dc.subject.classification	3404 Medicinal and biomolecular chemistry
dc.subject.mesh	Mice
dc.subject.mesh	Animals
dc.subject.mesh	Diet, High-Fat
dc.subject.mesh	Dysbiosis
dc.subject.mesh	Cytokines
dc.subject.mesh	Gastrointestinal Microbiome
dc.subject.mesh	Immunity
dc.subject.mesh	Mice, Inbred C57BL
dc.subject.mesh	Animals
dc.subject.mesh	Mice, Inbred C57BL
dc.subject.mesh	Mice
dc.subject.mesh	Cytokines
dc.subject.mesh	Immunity
dc.subject.mesh	Diet, High-Fat
dc.subject.mesh	Dysbiosis
dc.subject.mesh	Gastrointestinal Microbiome
dc.subject.mesh	Mice
dc.subject.mesh	Animals
dc.subject.mesh	Diet, High-Fat
dc.subject.mesh	Dysbiosis
dc.subject.mesh	Cytokines
dc.subject.mesh	Gastrointestinal Microbiome
dc.subject.mesh	Immunity
dc.subject.mesh	Mice, Inbred C57BL
dc.title	Irradiation-Induced Dysbiosis: The Compounding Effect of High-Fat Diet on Metabolic and Immune Functions in Mice.
dc.type	Journal Article
utslib.citation.volume	24
utslib.location.activity	Switzerland
utslib.for	0399 Other Chemical Sciences
utslib.for	0604 Genetics
utslib.for	0699 Other Biological Sciences
pubs.organisational-group	University of Technology Sydney
pubs.organisational-group	University of Technology Sydney/Strength - CFI - Centre for Inflammation
utslib.copyright.status	open_access	*
dc.date.updated	2024-03-27T02:17:03Z
pubs.issue	6
pubs.publication-status	Published online
pubs.volume	24
utslib.citation.issue	6

Abstract:

The negative impact of irradiation or diet on the metabolic and immune profiles of cancer survivors have been previously demonstrated. The gut microbiota plays a critical role in regulating these functions and is highly sensitive to cancer therapies. The aim of this study was to investigate the effect of irradiation and diet on the gut microbiota and metabolic or immune functions. We exposed C57Bl/6J mice to a single dose of 6 Gy radiation and after 5 weeks, fed them a chow or high-fat diet (HFD) for 12 weeks. We characterised their faecal microbiota, metabolic (whole body and adipose tissue) functions, and systemic (multiplex cytokine, chemokine assay, and immune cell profiling) and adipose tissue inflammatory profiles (immune cell profiling). At the end of the study, we observed a compounding effect of irradiation and diet on the metabolic and immune profiles of adipose tissue, with exposed mice fed a HFD displaying a greater inflammatory signature and impaired metabolism. Mice fed a HFD also showed altered microbiota, irrespective of irradiation status. An altered diet may exacerbate the detrimental effects of irradiation on both the metabolic and inflammatory profiles. This could have implications for the diagnosis and prevention of metabolic complications in cancer survivors exposed to radiation.

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http://hdl.handle.net/10453/177227