Developing Microfluidic Tools for Intracellular Delivery

Morshedi Rad, Dorsa

Developing Microfluidic Tools for Intracellular Delivery

Morshedi Rad, Dorsa

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Publication Type:: Thesis
Issue Date:: 2023

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Field	Value	Language
dc.contributor.author	Morshedi Rad, Dorsa
dc.date.accessioned	2024-04-03T03:37:04Z
dc.date.available	2024-04-03T03:37:04Z
dc.date.issued	2023
dc.identifier.uri	http://hdl.handle.net/10453/177463
dc.description	University of Technology Sydney. Faculty of Engineering and Information Technology.	en_US.UTF-8
dc.description.abstract	Intracellular delivery involves the introduction of biomolecules into the target cells. This process requires overcoming the plasma membrane barrier, which protects the intracellular compositions from outside the cell. Intracellular delivery is a powerful tool to monitor and decode cellular function and behavior, playing a pivotal role in biomedical research, biomanufacturing, and therapeutic applications. Current intracellular delivery technologies can transport cargo across the cell membrane using carrier-mediated or membrane disruption-mediated techniques. Recently, there has been renewed interest in microfluidic-based membrane disruption for payload delivery applications. Microfluidic technologies leverage electric fields, laser-induced shock waves, and hydrodynamic forces to induce rapid mechanical deformations and create transient membrane disruptions without causing permanent damage to the target cells. These techniques have shown success in genome editing of hard-to-transfect primary human hematopoietic stem cells (HSCs) and T cells. However, current microfluidic delivery techniques have certain limitations. These include inconsistent plasma membrane disruption, clogging, low throughput, uneven cargo delivery, and difficulties in delivering of large-sized gene editing tools such as plasmid DNA and CRISPR/Cas-9 ribonucleoprotein. This thesis is structured to demonstrate the development of novel microfluidic tools that enable efficient intracellular delivery of nanomaterials and CRISPR/Cas-9 plasmid DNA through membrane disruption while maintaining high cell viability.	en_US.UTF-8
dc.format	Thesis (PhD)
dc.language.iso	en_US	en_US.UTF-8
dc.relation	https://opus.lib.uts.edu.au/bitstream/10453/177463/1/thesis.pdf
dc.rights	info:eu-repo/semantics/embargoedAccess
dc.rights	The author owns the copyright in this thesis including all reproduction and reuse rights for the work. The work may not be altered without the permission of the copyright owner. Attribution is essential when quoting or paraphrasing from this thesis.
dc.rights	© 2023 Dorsa Morshedi Rad
dc.rights	au.edu.uts.lib/cph
dc.title	Developing Microfluidic Tools for Intracellular Delivery	en_US.UTF-8
dc.type	Thesis
utslib.copyright.status	embargoed	*
utslib.copyright.embargo	2025-12-31T00:00:00+1000

Abstract:

Intracellular delivery involves the introduction of biomolecules into the target cells. This process requires overcoming the plasma membrane barrier, which protects the intracellular compositions from outside the cell. Intracellular delivery is a powerful tool to monitor and decode cellular function and behavior, playing a pivotal role in biomedical research, biomanufacturing, and therapeutic applications. Current intracellular delivery technologies can transport cargo across the cell membrane using carrier-mediated or membrane disruption-mediated techniques. Recently, there has been renewed interest in microfluidic-based membrane disruption for payload delivery applications. Microfluidic technologies leverage electric fields, laser-induced shock waves, and hydrodynamic forces to induce rapid mechanical deformations and create transient membrane disruptions without causing permanent damage to the target cells. These techniques have shown success in genome editing of hard-to-transfect primary human hematopoietic stem cells (HSCs) and T cells. However, current microfluidic delivery techniques have certain limitations. These include inconsistent plasma membrane disruption, clogging, low throughput, uneven cargo delivery, and difficulties in delivering of large-sized gene editing tools such as plasmid DNA and CRISPR/Cas-9 ribonucleoprotein. This thesis is structured to demonstrate the development of novel microfluidic tools that enable efficient intracellular delivery of nanomaterials and CRISPR/Cas-9 plasmid DNA through membrane disruption while maintaining high cell viability.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/177463