Investigation of post-mortem lipids in textiles associated with decomposing remains

Publication Type:
Thesis
Issue Date:
2023
Full metadata record
After death, the human body undergoes decomposition, releasing fluids comprising water, protein, lipids, carbohydrates and minerals into the surrounding environment. Lipids, due to their hydrophobic nature, can endure harsh conditions, longer than other decomposition components. Archaeologists have thus targeted lipids in a variety of matrices, including clothing, to reconstruct the history of human civilization across the globe. In a forensic context, clothing at crime scenes is crucial evidence, and applying archaeological lipid analysis can assist investigators in complex death investigations, aiding in event reconstruction and time since death estimations. To study post-mortem lipids in textiles, pig and human remains were clothed and left to decompose at the Australian Facility for Taphonomic Experimental Research (AFTER). Clothing samples were initially analyzed using attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy. Further lipid profile elucidation was conducted through gas chromatography coupled with tandem mass spectrometry (GC-MS/MS) on extracted samples. This project demonstrated the utility of post-mortem lipids in conjunction with decomposition timelines, additionally uncovering interspecies differences. Lipids in textiles show promise in forensic science, offering an additional avenue for estimating time since death and providing crucial insights for solving cases involving human remains.
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