Profiling extracellular vesicle surface proteins with 10 µL peripheral plasma within 4 h.
- Publisher:
- WILEY
- Publication Type:
- Journal Article
- Citation:
- J Extracell Vesicles, 2023, 12, (9), pp. e12364
- Issue Date:
- 2023-09
Open Access
Copyright Clearance Process
- Recently Added
- In Progress
- Open Access
This item is open access.
Full metadata record
Field | Value | Language |
---|---|---|
dc.contributor.author | He, J | |
dc.contributor.author | Li, H | |
dc.contributor.author | Mai, J | |
dc.contributor.author | Ke, Y | |
dc.contributor.author | Zhai, C | |
dc.contributor.author | Li, JJ | |
dc.contributor.author | Jiang, L | |
dc.contributor.author | Shen, G | |
dc.contributor.author | Ding, X | |
dc.date.accessioned | 2024-04-29T08:03:25Z | |
dc.date.available | 2023-08-22 | |
dc.date.available | 2024-04-29T08:03:25Z | |
dc.date.issued | 2023-09 | |
dc.identifier.citation | J Extracell Vesicles, 2023, 12, (9), pp. e12364 | |
dc.identifier.issn | 2001-3078 | |
dc.identifier.issn | 2001-3078 | |
dc.identifier.uri | http://hdl.handle.net/10453/178462 | |
dc.description.abstract | Extracellular vesicle (EV) surface proteins, expressed by primary tumours, are important biomarkers for early cancer diagnosis. However, the detection of these EV proteins is complicated by their low abundance and interference from non-EV components in clinical samples. Herein, we present a MEmbrane-Specific Separation and two-step Cascade AmpLificatioN (MESS2CAN) strategy for direct detection of EV surface proteins within 4 h. MESS2CAN utilises novel lipid probes (long chains linked by PEG2K with biotin at one end, and DSPE at the other end) and streptavidin-coated magnetic beads, permitting a 49.6% EV recovery rate within 1 h. A dual amplification strategy with a primer exchange reaction (PER) cascaded by the Cas12a system then allows sensitive detection of the target protein at 10 EV particles per microliter. Using 4 cell lines and 90 clinical test samples, we demonstrate MESS2CAN for analysing HER2, EpCAM and EGFR expression on EVs derived from cells and patient plasma. MESS2CAN reports the desired specificity and sensitivity of EGFR (AUC = 0.98) and of HER2 (AUC = 1) for discriminating between HER2-positive breast cancer, triple-negative breast cancer and healthy donors. MESS2CAN is a pioneering method for highly sensitive in vitro EV diagnostics, applicable to clinical samples with trace amounts of EVs. | |
dc.format | ||
dc.language | eng | |
dc.publisher | WILEY | |
dc.relation.ispartof | J Extracell Vesicles | |
dc.relation.isbasedon | 10.1002/jev2.12364 | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject | 0601 Biochemistry and Cell Biology | |
dc.subject.classification | 3101 Biochemistry and cell biology | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Female | |
dc.subject.mesh | Membrane Proteins | |
dc.subject.mesh | Extracellular Vesicles | |
dc.subject.mesh | Biotin | |
dc.subject.mesh | Breast Neoplasms | |
dc.subject.mesh | ErbB Receptors | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Breast Neoplasms | |
dc.subject.mesh | Biotin | |
dc.subject.mesh | Membrane Proteins | |
dc.subject.mesh | Female | |
dc.subject.mesh | ErbB Receptors | |
dc.subject.mesh | Extracellular Vesicles | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Female | |
dc.subject.mesh | Membrane Proteins | |
dc.subject.mesh | Extracellular Vesicles | |
dc.subject.mesh | Biotin | |
dc.subject.mesh | Breast Neoplasms | |
dc.subject.mesh | ErbB Receptors | |
dc.title | Profiling extracellular vesicle surface proteins with 10 µL peripheral plasma within 4 h. | |
dc.type | Journal Article | |
utslib.citation.volume | 12 | |
utslib.location.activity | United States | |
utslib.for | 0601 Biochemistry and Cell Biology | |
pubs.organisational-group | University of Technology Sydney | |
pubs.organisational-group | University of Technology Sydney/Faculty of Engineering and Information Technology | |
pubs.organisational-group | University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering | |
utslib.copyright.status | open_access | * |
dc.date.updated | 2024-04-29T08:03:19Z | |
pubs.issue | 9 | |
pubs.publication-status | Published | |
pubs.volume | 12 | |
utslib.citation.issue | 9 |
Abstract:
Extracellular vesicle (EV) surface proteins, expressed by primary tumours, are important biomarkers for early cancer diagnosis. However, the detection of these EV proteins is complicated by their low abundance and interference from non-EV components in clinical samples. Herein, we present a MEmbrane-Specific Separation and two-step Cascade AmpLificatioN (MESS2CAN) strategy for direct detection of EV surface proteins within 4 h. MESS2CAN utilises novel lipid probes (long chains linked by PEG2K with biotin at one end, and DSPE at the other end) and streptavidin-coated magnetic beads, permitting a 49.6% EV recovery rate within 1 h. A dual amplification strategy with a primer exchange reaction (PER) cascaded by the Cas12a system then allows sensitive detection of the target protein at 10 EV particles per microliter. Using 4 cell lines and 90 clinical test samples, we demonstrate MESS2CAN for analysing HER2, EpCAM and EGFR expression on EVs derived from cells and patient plasma. MESS2CAN reports the desired specificity and sensitivity of EGFR (AUC = 0.98) and of HER2 (AUC = 1) for discriminating between HER2-positive breast cancer, triple-negative breast cancer and healthy donors. MESS2CAN is a pioneering method for highly sensitive in vitro EV diagnostics, applicable to clinical samples with trace amounts of EVs.
Please use this identifier to cite or link to this item:
Download statistics for the last 12 months
Not enough data to produce graph