Effect of Degree of Deacetylation of Chitosan/Chitin on Human Neural Stem Cell Culture.

Chang, F-C; Zhou, Y; James, MM; Zareie, HM; Ando, Y; Yang, J; Zhang, M

Effect of Degree of Deacetylation of Chitosan/Chitin on Human Neural Stem Cell Culture.

Chang, F-C Zhou, Y James, MM Zareie, HM Ando, Y Yang, J Zhang, M

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Publisher:: Wiley
Publication Type:: Journal Article
Citation:: Macromol Biosci, 2023, 23, (1), pp. e2200389
Issue Date:: 2023-01

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Field	Value	Language
dc.contributor.author	Chang, F-C
dc.contributor.author	Zhou, Y
dc.contributor.author	James, MM
dc.contributor.author	Zareie, HM
dc.contributor.author	Ando, Y
dc.contributor.author	Yang, J
dc.contributor.author	Zhang, M
dc.date.accessioned	2024-05-02T22:32:01Z
dc.date.available	2024-05-02T22:32:01Z
dc.date.issued	2023-01
dc.identifier.citation	Macromol Biosci, 2023, 23, (1), pp. e2200389
dc.identifier.issn	1616-5187
dc.identifier.issn	1616-5195
dc.identifier.uri	http://hdl.handle.net/10453/178553
dc.description.abstract	Stem cell therapy and research for neural diseases depends on reliable reproduction of neural stem cells. Chitosan-based materials have been proposed as a substrate for culturing human neural stem cells (hNSCs) in the pursuit of clinically compatible culture conditions that are chemically defined and compliant with good manufacturing practices. The physical and biochemical properties of chitosan and chitin are strongly regulated by the degree of deacetylation (DD). However, the effect of DD on hNSC behavior has not been systematically investigated. In this study, films with DD ranging from 93% to 14% are fabricated with chitosan and chitin. Under xeno-free conditions, hNSCs proliferate preferentially on films with a higher DD, exhibiting adherent morphology and retaining multipotency. Lowering the DD leads to formation of neural stem cell spheroids due to unsteady adhesion. The neural spheroids present NSC multipotency protein expression reduction and cytoplasmic translocation. This study provides an insight into the influence of the DD on hNSCs behavior and may serve as a guideline for hNSC research using chitosan-based biomaterials. It demonstrates the capability of controlling hNSC fate by simply tailoring the DD of chitosan.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	Wiley
dc.relation.ispartof	Macromol Biosci
dc.relation.isbasedon	10.1002/mabi.202200389
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0303 Macromolecular and Materials Chemistry, 0903 Biomedical Engineering, 0904 Chemical Engineering
dc.subject.classification	Polymers
dc.subject.classification	3403 Macromolecular and materials chemistry
dc.subject.classification	4003 Biomedical engineering
dc.subject.mesh	Humans
dc.subject.mesh	Chitosan
dc.subject.mesh	Chitin
dc.subject.mesh	Biocompatible Materials
dc.subject.mesh	Neural Stem Cells
dc.subject.mesh	Humans
dc.subject.mesh	Chitin
dc.subject.mesh	Chitosan
dc.subject.mesh	Biocompatible Materials
dc.subject.mesh	Neural Stem Cells
dc.subject.mesh	Humans
dc.subject.mesh	Chitosan
dc.subject.mesh	Chitin
dc.subject.mesh	Biocompatible Materials
dc.subject.mesh	Neural Stem Cells
dc.title	Effect of Degree of Deacetylation of Chitosan/Chitin on Human Neural Stem Cell Culture.
dc.type	Journal Article
utslib.citation.volume	23
utslib.location.activity	Germany
utslib.for	0303 Macromolecular and Materials Chemistry
utslib.for	0903 Biomedical Engineering
utslib.for	0904 Chemical Engineering
utslib.copyright.status	closed_access	*
dc.date.updated	2024-05-02T22:31:59Z
pubs.issue	1
pubs.publication-status	Published
pubs.volume	23
utslib.citation.issue	1

Abstract:

Stem cell therapy and research for neural diseases depends on reliable reproduction of neural stem cells. Chitosan-based materials have been proposed as a substrate for culturing human neural stem cells (hNSCs) in the pursuit of clinically compatible culture conditions that are chemically defined and compliant with good manufacturing practices. The physical and biochemical properties of chitosan and chitin are strongly regulated by the degree of deacetylation (DD). However, the effect of DD on hNSC behavior has not been systematically investigated. In this study, films with DD ranging from 93% to 14% are fabricated with chitosan and chitin. Under xeno-free conditions, hNSCs proliferate preferentially on films with a higher DD, exhibiting adherent morphology and retaining multipotency. Lowering the DD leads to formation of neural stem cell spheroids due to unsteady adhesion. The neural spheroids present NSC multipotency protein expression reduction and cytoplasmic translocation. This study provides an insight into the influence of the DD on hNSCs behavior and may serve as a guideline for hNSC research using chitosan-based biomaterials. It demonstrates the capability of controlling hNSC fate by simply tailoring the DD of chitosan.

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http://hdl.handle.net/10453/178553