Protective effect of L‑carnitine against oxidative stress injury in human ovarian granulosa cells.

Li, X; Wu, X; Ma, T; Zhang, Y; Sun, P; Qi, D; Ma, H

Protective effect of L‑carnitine against oxidative stress injury in human ovarian granulosa cells.

Li, X Wu, X Ma, T Zhang, Y Sun, P Qi, D Ma, H

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Publisher:: Spandidos Publications
Publication Type:: Journal Article
Citation:: Exp Ther Med, 2023, 25, (4), pp. 161
Issue Date:: 2023-04

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Field	Value	Language
dc.contributor.author	Li, X
dc.contributor.author	Wu, X
dc.contributor.author	Ma, T
dc.contributor.author	Zhang, Y
dc.contributor.author	Sun, P
dc.contributor.author	Qi, D
dc.contributor.author	Ma, H
dc.date.accessioned	2024-05-24T05:00:27Z
dc.date.available	2022-10-31
dc.date.available	2024-05-24T05:00:27Z
dc.date.issued	2023-04
dc.identifier.citation	Exp Ther Med, 2023, 25, (4), pp. 161
dc.identifier.issn	1792-0981
dc.identifier.issn	1792-1015
dc.identifier.uri	http://hdl.handle.net/10453/179183
dc.description.abstract	Granulosa cells (GCs) are important for supporting and nourishing oocytes during follicular development and maturation. Oxidative stress (OS) injury of GCs can lead to decreased responsiveness of follicles to follicular stimulating hormone (FSH), which will accelerate ovarian senescence and adversely affect oocyte and embryo quality. Since L-carnitine has been previously reported to exert strong antioxidant activity, the present study aimed to explore the possible effects of L-carnitine on OS injury and FSH receptor (FSHR) expression in ovarian GCs, results of which may be of significance for GCs protection. In the present study, OS was induced in vitro in KGN cells by treatment with H2O2. KGN cells were cultured and divided into the following four groups: Blank, OS, and 40 and 80 µmol/l L-carnitine pre-treatment groups. In the OS group, cells showed nuclear pyknosis, mitochondria swelled irregularly whilst featuring fractured cristae. In addition, cell viability, ROS levels, superoxide dismutase levels, glutathione levels, malondialdehyde levels, the mitochondrial membrane potential and FSHR expression, as determined by Cell Counting Kit-8 (CCK-8), 2,7-dichloro-dihydrofluorescein diacetate, spectrophotometry, ELISA, spectrophotometry, JC-1 and western blot analyses, respectively, were all significantly different in the OS group compared with those in the control group. However, malonaldehyde levels, reactive oxygen species levels and the apoptosis rate according to flow cytometry were all significantly increased compared with those in the control. Compared with those in the OS group, the morphology of cells and mitochondria in the L-carnitine pre-treatment groups were improved, whilst cell viability and the expression of FSHR were significantly increased but oxidative stress injury was decreased. The present results suggest that L-carnitine can protect the cells from OS damage induced by H2O2, enhance antioxidant activity whilst suppressing the apoptosis of GCs, in addition to preserving FSHR expression in GCs under OS. Therefore, the present study revealed that the introduction of L-carnitine in clinical medicine or dietary supplement may protect GCs, improve follicular quality and female reproductive function.
dc.format	Electronic-eCollection
dc.language	eng
dc.publisher	Spandidos Publications
dc.relation.ispartof	Exp Ther Med
dc.relation.isbasedon	10.3892/etm.2023.11860
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	1104 Complementary and Alternative Medicine, 1111 Nutrition and Dietetics
dc.subject.classification	3214 Pharmacology and pharmaceutical sciences
dc.subject.classification	4208 Traditional, complementary and integrative medicine
dc.title	Protective effect of L‑carnitine against oxidative stress injury in human ovarian granulosa cells.
dc.type	Journal Article
utslib.citation.volume	25
utslib.location.activity	Greece
utslib.for	1104 Complementary and Alternative Medicine
utslib.for	1111 Nutrition and Dietetics
utslib.copyright.status	open_access	*
dc.date.updated	2024-05-24T05:00:21Z
pubs.issue	4
pubs.publication-status	Published online
pubs.volume	25
utslib.citation.issue	4

Abstract:

Granulosa cells (GCs) are important for supporting and nourishing oocytes during follicular development and maturation. Oxidative stress (OS) injury of GCs can lead to decreased responsiveness of follicles to follicular stimulating hormone (FSH), which will accelerate ovarian senescence and adversely affect oocyte and embryo quality. Since L-carnitine has been previously reported to exert strong antioxidant activity, the present study aimed to explore the possible effects of L-carnitine on OS injury and FSH receptor (FSHR) expression in ovarian GCs, results of which may be of significance for GCs protection. In the present study, OS was induced in vitro in KGN cells by treatment with H2O2. KGN cells were cultured and divided into the following four groups: Blank, OS, and 40 and 80 µmol/l L-carnitine pre-treatment groups. In the OS group, cells showed nuclear pyknosis, mitochondria swelled irregularly whilst featuring fractured cristae. In addition, cell viability, ROS levels, superoxide dismutase levels, glutathione levels, malondialdehyde levels, the mitochondrial membrane potential and FSHR expression, as determined by Cell Counting Kit-8 (CCK-8), 2,7-dichloro-dihydrofluorescein diacetate, spectrophotometry, ELISA, spectrophotometry, JC-1 and western blot analyses, respectively, were all significantly different in the OS group compared with those in the control group. However, malonaldehyde levels, reactive oxygen species levels and the apoptosis rate according to flow cytometry were all significantly increased compared with those in the control. Compared with those in the OS group, the morphology of cells and mitochondria in the L-carnitine pre-treatment groups were improved, whilst cell viability and the expression of FSHR were significantly increased but oxidative stress injury was decreased. The present results suggest that L-carnitine can protect the cells from OS damage induced by H2O2, enhance antioxidant activity whilst suppressing the apoptosis of GCs, in addition to preserving FSHR expression in GCs under OS. Therefore, the present study revealed that the introduction of L-carnitine in clinical medicine or dietary supplement may protect GCs, improve follicular quality and female reproductive function.

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