Enhancing the Characterisation of the Phosphatidylinositols

Gertner, David

Enhancing the Characterisation of the Phosphatidylinositols

Gertner, David

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Publication Type:: Thesis
Issue Date:: 2023

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Field	Value	Language
dc.contributor.author	Gertner, David
dc.date.accessioned	2024-06-18T03:52:09Z
dc.date.available	2024-06-18T03:52:09Z
dc.date.issued	2023
dc.identifier.uri	http://hdl.handle.net/10453/179547
dc.description	University of Technology Sydney. Faculty of Science.	en_US.UTF-8
dc.description.abstract	The phosphatidylinositols are an interesting and diverse group of molecules. They’re ubiquitous throughout known life and are involved in almost every aspect of a cell's life and death. Consequently, they are also of significant interest as biomarkers, as deviations in their lipidomic profiles are associated with the development of many serious pathologies. Despite this, the methods and tools used to characterise these lipids are limited, as they provide only a shallow glimpse at the distribution of the molecular species present. To enhance the future characterisation of these molecules, a re-evaluation of current lipidomic protocols may be necessary to favour their analysis. This thesis outlines the of steps taken to this end, where a series of advancements were made. As a result of the work done here, 148 unique lyso- phosphatidylinositol, phosphatidylinositol, and phosphatidylinositol-phosphate species were identified in four cultivars of canola, permitting differentiation based on their unique profiles. To date, this represents the most comprehensive characterisation of phosphatidylinositol species in any matrix.	en_US.UTF-8
dc.format	Thesis (PhD)
dc.language.iso	en_US	en_US.UTF-8
dc.relation	https://opus.lib.uts.edu.au/bitstream/10453/179547/1/thesis.pdf
dc.rights	info:eu-repo/semantics/openAccess
dc.rights	The author owns the copyright in this thesis including all reproduction and reuse rights for the work. The work may not be altered without the permission of the copyright owner. Attribution is essential when quoting or paraphrasing from this thesis.
dc.rights	© 2023 David Gertner
dc.rights	au.edu.uts.lib/nph
dc.title	Enhancing the Characterisation of the Phosphatidylinositols	en_US.UTF-8
dc.type	Thesis
utslib.copyright.status	open_access	*

Abstract:

The phosphatidylinositols are an interesting and diverse group of molecules. They’re ubiquitous throughout known life and are involved in almost every aspect of a cell's life and death. Consequently, they are also of significant interest as biomarkers, as deviations in their lipidomic profiles are associated with the development of many serious pathologies. Despite this, the methods and tools used to characterise these lipids are limited, as they provide only a shallow glimpse at the distribution of the molecular species present. To enhance the future characterisation of these molecules, a re-evaluation of current lipidomic protocols may be necessary to favour their analysis. This thesis outlines the of steps taken to this end, where a series of advancements were made. As a result of the work done here, 148 unique lyso- phosphatidylinositol, phosphatidylinositol, and phosphatidylinositol-phosphate species were identified in four cultivars of canola, permitting differentiation based on their unique profiles. To date, this represents the most comprehensive characterisation of phosphatidylinositol species in any matrix.

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http://hdl.handle.net/10453/179547