Distillation Extraction of Prunella vulgaris Volatile Organic Compounds: Characterization and their Anti-tumoral effects

Mak, William Chi Keung

Distillation Extraction of Prunella vulgaris Volatile Organic Compounds: Characterization and their Anti-tumoral effects

Mak, William Chi Keung

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Publication Type:: Thesis
Issue Date:: 2023

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Field	Value	Language
dc.contributor.author	Mak, William Chi Keung
dc.date.accessioned	2024-06-19T00:30:58Z
dc.date.available	2024-06-19T00:30:58Z
dc.date.issued	2023
dc.identifier.uri	http://hdl.handle.net/10453/179558
dc.description	University of Technology Sydney. Faculty of Science.	en_US.UTF-8
dc.description.abstract	The thesis consists of two parts. The first part reviews the research work of the past three decades on the anti-tumoral and anti-viral activities of the herb Prunella vulgaris L. (PV). The second part explores experimentally the volatile organic compounds (VOCs) of PV. The study includes the characterization of the steam distillation process which extracted the volatile chemical constituents from the herb PV. The characteristics of the VOCs extracted during the distillation process were explored and reported. It was found that VOCs emerged from the herb constantly at approximately the same rates and continuously for a long time. Abundances of typical compounds obtained from the steam distillation process were compared with those obtained from the hydro distillation process and, the dynamics of the extraction process was proposed and verified. It was found that the hydro distillation process is a more efficient extraction method than steam distillation. Then, the pharmacological effect, specifically the anti-tumoral effect, of the VOCs of PV was studied, with a colorimetric assay using a tetrazolium-based reagent to probe the cytotoxicity of the volatile compounds of PV on the cancer cells from the cell line SCC154. The experiments confirmed the cytotoxicity of the VOCs of PV against the cancer cells. As the extraction of the volatile compounds was explored for the potential of drug production, and the manufacturing process took time, the aging effect on the abundances of the volatile compounds was examined. It was found that the cytotoxicity of the herb extracts persisted for up to eight weeks. The effect of the change of the steam flow rate on the abundances of the extracted volatile compounds was also investigated to further explore the dynamics of the extraction process. The findings in the experiments give great insights into the design of a drug manufacture process which makes use of the VOCs from PV.	en_US.UTF-8
dc.format	Thesis (PhD)
dc.language.iso	en_US	en_US.UTF-8
dc.relation	https://opus.lib.uts.edu.au/bitstream/10453/179558/1/thesis.pdf
dc.rights	info:eu-repo/semantics/openAccess
dc.rights	The author owns the copyright in this thesis including all reproduction and reuse rights for the work. The work may not be altered without the permission of the copyright owner. Attribution is essential when quoting or paraphrasing from this thesis.
dc.rights	© 2023 William Chi Keung Mak
dc.rights	au.edu.uts.lib/cph
dc.title	Distillation Extraction of Prunella vulgaris Volatile Organic Compounds: Characterization and their Anti-tumoral effects	en_US.UTF-8
dc.type	Thesis
utslib.copyright.status	open_access	*

Abstract:

The thesis consists of two parts. The first part reviews the research work of the past three decades on the anti-tumoral and anti-viral activities of the herb Prunella vulgaris L. (PV). The second part explores experimentally the volatile organic compounds (VOCs) of PV. The study includes the characterization of the steam distillation process which extracted the volatile chemical constituents from the herb PV. The characteristics of the VOCs extracted during the distillation process were explored and reported. It was found that VOCs emerged from the herb constantly at approximately the same rates and continuously for a long time. Abundances of typical compounds obtained from the steam distillation process were compared with those obtained from the hydro distillation process and, the dynamics of the extraction process was proposed and verified. It was found that the hydro distillation process is a more efficient extraction method than steam distillation. Then, the pharmacological effect, specifically the anti-tumoral effect, of the VOCs of PV was studied, with a colorimetric assay using a tetrazolium-based reagent to probe the cytotoxicity of the volatile compounds of PV on the cancer cells from the cell line SCC154. The experiments confirmed the cytotoxicity of the VOCs of PV against the cancer cells. As the extraction of the volatile compounds was explored for the potential of drug production, and the manufacturing process took time, the aging effect on the abundances of the volatile compounds was examined. It was found that the cytotoxicity of the herb extracts persisted for up to eight weeks. The effect of the change of the steam flow rate on the abundances of the extracted volatile compounds was also investigated to further explore the dynamics of the extraction process. The findings in the experiments give great insights into the design of a drug manufacture process which makes use of the VOCs from PV.

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http://hdl.handle.net/10453/179558