Droplet 3D cryobioprinting for fabrication of free-standing and volumetric structures

Weygant, J; Entezari, A; Koch, F; Galaviz, RA; Garciamendez, CE; Hernández, P; Ortiz, V; Ruiz, DSR; Aguilar, F; Andolfi, A; Cai, L; Maharjan, S; Osorio, A; Zhang, YS

Droplet 3D cryobioprinting for fabrication of free-standing and volumetric structures

Weygant, J Entezari, A

Koch, F Galaviz, RA Garciamendez, CE Hernández, P Ortiz, V Ruiz, DSR Aguilar, F Andolfi, A Cai, L Maharjan, S Osorio, A Zhang, YS

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Publisher:: Wiley
Publication Type:: Journal Article
Citation:: Aggregate, 2024
Issue Date:: 2024-01-01

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Field	Value	Language
dc.contributor.author	Weygant, J
dc.contributor.author	Entezari, A https://orcid.org/0000-0003-0504-8364
dc.contributor.author	Koch, F
dc.contributor.author	Galaviz, RA
dc.contributor.author	Garciamendez, CE
dc.contributor.author	Hernández, P
dc.contributor.author	Ortiz, V
dc.contributor.author	Ruiz, DSR
dc.contributor.author	Aguilar, F
dc.contributor.author	Andolfi, A
dc.contributor.author	Cai, L
dc.contributor.author	Maharjan, S
dc.contributor.author	Osorio, A
dc.contributor.author	Zhang, YS
dc.date.accessioned	2024-09-04T01:19:46Z
dc.date.available	2024-09-04T01:19:46Z
dc.date.issued	2024-01-01
dc.identifier.citation	Aggregate, 2024
dc.identifier.issn	2766-8541
dc.identifier.issn	2692-4560
dc.identifier.uri	http://hdl.handle.net/10453/180608
dc.description.abstract	Droplet-based bioprinting has shown remarkable potential in tissue engineering and regenerative medicine. However, it requires bioinks with low viscosities, which makes it challenging to create complex 3D structures and spatially pattern them with different materials. This study introduces a novel approach to bioprinting sophisticated volumetric objects by merging droplet-based bioprinting and cryobioprinting techniques. By leveraging the benefits of cryopreservation, we fabricated, for the first time, intricate, self-supporting cell-free or cell-laden structures with single or multiple materials in a simple droplet-based bioprinting process that is facilitated by depositing the droplets onto a cryoplate followed by crosslinking during revival. The feasibility of this approach is demonstrated by bioprinting several cell types, with cell viability increasing to 80%–90% after up to 2 or 3 weeks of culture. Furthermore, the applicational capabilities of this approach are showcased by bioprinting an endothelialized breast cancer model. The results indicate that merging droplet and cryogenic bioprinting complements current droplet-based bioprinting techniques and opens new avenues for the fabrication of volumetric objects with enhanced complexity and functionality, presenting exciting potential for biomedical applications.
dc.language	en
dc.publisher	Wiley
dc.relation.ispartof	Aggregate
dc.relation.isbasedon	10.1002/agt2.599
dc.rights	info:eu-repo/semantics/openAccess
dc.title	Droplet 3D cryobioprinting for fabrication of free-standing and volumetric structures
dc.type	Journal Article
pubs.organisational-group	University of Technology Sydney
pubs.organisational-group	University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
utslib.copyright.status	open_access	*
dc.rights.license	This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0). To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/
dc.date.updated	2024-09-04T01:19:43Z
pubs.publication-status	Published

Abstract:

Droplet-based bioprinting has shown remarkable potential in tissue engineering and regenerative medicine. However, it requires bioinks with low viscosities, which makes it challenging to create complex 3D structures and spatially pattern them with different materials. This study introduces a novel approach to bioprinting sophisticated volumetric objects by merging droplet-based bioprinting and cryobioprinting techniques. By leveraging the benefits of cryopreservation, we fabricated, for the first time, intricate, self-supporting cell-free or cell-laden structures with single or multiple materials in a simple droplet-based bioprinting process that is facilitated by depositing the droplets onto a cryoplate followed by crosslinking during revival. The feasibility of this approach is demonstrated by bioprinting several cell types, with cell viability increasing to 80%–90% after up to 2 or 3 weeks of culture. Furthermore, the applicational capabilities of this approach are showcased by bioprinting an endothelialized breast cancer model. The results indicate that merging droplet and cryogenic bioprinting complements current droplet-based bioprinting techniques and opens new avenues for the fabrication of volumetric objects with enhanced complexity and functionality, presenting exciting potential for biomedical applications.

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http://hdl.handle.net/10453/180608