Single-cell laser emitting cytometry for label-free nucleolus fingerprinting.

Fang, G; Qiao, Z; Huang, L; Zhu, H; Xie, J; Zhou, T; Xiong, Z; Su, I-H; Jin, D; Chen, Y-C

Single-cell laser emitting cytometry for label-free nucleolus fingerprinting.

Fang, G Qiao, Z Huang, L Zhu, H Xie, J Zhou, T Xiong, Z Su, I-H Jin, D

Chen, Y-C

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Publisher:: NATURE PORTFOLIO
Publication Type:: Journal Article
Citation:: Nat Commun, 2024, 15, (1), pp. 7332
Issue Date:: 2024-08-26

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Field	Value	Language
dc.contributor.author	Fang, G
dc.contributor.author	Qiao, Z
dc.contributor.author	Huang, L
dc.contributor.author	Zhu, H
dc.contributor.author	Xie, J
dc.contributor.author	Zhou, T
dc.contributor.author	Xiong, Z
dc.contributor.author	Su, I-H
dc.contributor.author	Jin, D https://orcid.org/0000-0003-1046-2666
dc.contributor.author	Chen, Y-C
dc.date.accessioned	2024-10-09T01:12:22Z
dc.date.available	2024-08-09
dc.date.available	2024-10-09T01:12:22Z
dc.date.issued	2024-08-26
dc.identifier.citation	Nat Commun, 2024, 15, (1), pp. 7332
dc.identifier.issn	2041-1723
dc.identifier.issn	2041-1723
dc.identifier.uri	http://hdl.handle.net/10453/181290
dc.description.abstract	The nucleolus, a recognized biomolecular condensate, serves as the hub for ribosome biogenesis within the cell nucleus. Its quantity and morphology are discernible indicators of cellular functional states. However, precise identification and quantification of nucleoli remain challenging without specific labeling, particularly for suspended cells, tissue-level analysis and high-throughput applications. Here we introduce a single-cell laser emitting cytometry (SLEC) for label-free nucleolus differentiation through light-matter interactions within a Fabry-Perot resonator. The separated gain medium enhances the threshold difference by 36-fold between nucleolus and its surroundings, enabling selective laser emissions at nucleolar area while maintaining lower-order mode. The laser emission image provides insights into structural inhomogeneity, temporal fluid-like dynamics, and pathological application. Lasing spectral fingerprint depicts the quantity and size of nucleoli within a single cell, showcasing the label-free flow cytometry for nucleolus. This approach holds promise for nucleolus-guided cell screening and drug evaluation, advancing the study of diseases such as cancer and neurodegenerative disorders.
dc.format	Electronic
dc.language	eng
dc.publisher	NATURE PORTFOLIO
dc.relation.ispartof	Nat Commun
dc.relation.isbasedon	10.1038/s41467-024-51574-5
dc.rights	info:eu-repo/semantics/openAccess
dc.subject.mesh	Cell Nucleolus
dc.subject.mesh	Single-Cell Analysis
dc.subject.mesh	Humans
dc.subject.mesh	Flow Cytometry
dc.subject.mesh	Lasers
dc.subject.mesh	HeLa Cells
dc.subject.mesh	Hela Cells
dc.subject.mesh	Cell Nucleolus
dc.subject.mesh	Humans
dc.subject.mesh	Flow Cytometry
dc.subject.mesh	Lasers
dc.subject.mesh	Single-Cell Analysis
dc.subject.mesh	Cell Nucleolus
dc.subject.mesh	Single-Cell Analysis
dc.subject.mesh	Humans
dc.subject.mesh	Flow Cytometry
dc.subject.mesh	Lasers
dc.subject.mesh	HeLa Cells
dc.title	Single-cell laser emitting cytometry for label-free nucleolus fingerprinting.
dc.type	Journal Article
utslib.citation.volume	15
utslib.location.activity	England
pubs.organisational-group	University of Technology Sydney
pubs.organisational-group	University of Technology Sydney/Faculty of Science
pubs.organisational-group	University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	University of Technology Sydney/UTS Groups
pubs.organisational-group	University of Technology Sydney/UTS Groups/Institute of Biomedical Materials and Devices (IBMD)
utslib.copyright.status	open_access	*
dc.rights.license	This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND 4.0). To view a copy of this license, visit https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.date.updated	2024-10-09T01:12:14Z
pubs.issue	1
pubs.publication-status	Published online
pubs.volume	15
utslib.citation.issue	1

Abstract:

The nucleolus, a recognized biomolecular condensate, serves as the hub for ribosome biogenesis within the cell nucleus. Its quantity and morphology are discernible indicators of cellular functional states. However, precise identification and quantification of nucleoli remain challenging without specific labeling, particularly for suspended cells, tissue-level analysis and high-throughput applications. Here we introduce a single-cell laser emitting cytometry (SLEC) for label-free nucleolus differentiation through light-matter interactions within a Fabry-Perot resonator. The separated gain medium enhances the threshold difference by 36-fold between nucleolus and its surroundings, enabling selective laser emissions at nucleolar area while maintaining lower-order mode. The laser emission image provides insights into structural inhomogeneity, temporal fluid-like dynamics, and pathological application. Lasing spectral fingerprint depicts the quantity and size of nucleoli within a single cell, showcasing the label-free flow cytometry for nucleolus. This approach holds promise for nucleolus-guided cell screening and drug evaluation, advancing the study of diseases such as cancer and neurodegenerative disorders.

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http://hdl.handle.net/10453/181290