Improved effectiveness of X-PDT against human triple-negative breast cancer cells through the use of liposomes co-loaded with protoporphyrin IX and perfluorooctyl bromide.

Yang, B; Sang, R; Li, Y; Goldys, EM; Deng, W

Improved effectiveness of X-PDT against human triple-negative breast cancer cells through the use of liposomes co-loaded with protoporphyrin IX and perfluorooctyl bromide.

Yang, B Sang, R Li, Y Goldys, EM Deng, W

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Publisher:: Royal Society of Chemistry (RSC)
Publication Type:: Journal Article
Citation:: J Mater Chem B, 2024, 12, (15), pp. 3764-3773
Issue Date:: 2024-04-17

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Field	Value	Language
dc.contributor.author	Yang, B
dc.contributor.author	Sang, R
dc.contributor.author	Li, Y
dc.contributor.author	Goldys, EM
dc.contributor.author	Deng, W https://orcid.org/0000-0002-9413-0978
dc.date.accessioned	2024-11-04T01:26:14Z
dc.date.available	2024-11-04T01:26:14Z
dc.date.issued	2024-04-17
dc.identifier.citation	J Mater Chem B, 2024, 12, (15), pp. 3764-3773
dc.identifier.issn	2050-750X
dc.identifier.issn	2050-7518
dc.identifier.uri	http://hdl.handle.net/10453/181693
dc.description.abstract	In this study, we utilized X-ray-induced photodynamic therapy (X-PDT) against triple-negative breast cancer (TNBC) cells. To achieve this, we developed a liposome delivery system that co-loaded protoporphyrin IX (PPIX) and perfluorooctyl bromide (PFOB) in a rational manner. Low-dose X-ray at 2 Gy was employed to activate PPIX for the generation of reactive oxygen species (ROS), and the co-loading of PFOB provided additional oxygen to enhance ROS production. The resulting highly toxic ROS effectively induced cell death in TNBC. In vitro X-PDT effects, including intracellular ROS generation, cell viability, and apoptosis/necrosis assays in TNBC cells, were thoroughly investigated. Our results indicate that the nanocarriers effectively induced X-PDT effects with very low-dose radiation, making it feasible to damage cancer cells. This suggests the potential for the effective utilization of X-PDT in treating hypoxic cancers, including TNBC, with only a fraction of conventional radiotherapy.
dc.format	Electronic
dc.language	eng
dc.publisher	Royal Society of Chemistry (RSC)
dc.relation	http://purl.org/au-research/grants/arc/CE140100003
dc.relation	http://purl.org/au-research/grants/nhmrc/1181889
dc.relation.ispartof	J Mater Chem B
dc.relation.isbasedon	10.1039/d4tb00011k
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0303 Macromolecular and Materials Chemistry, 0903 Biomedical Engineering
dc.subject.classification	3403 Macromolecular and materials chemistry
dc.subject.classification	4003 Biomedical engineering
dc.subject.classification	4004 Chemical engineering
dc.subject.mesh	Humans
dc.subject.mesh	Photochemotherapy
dc.subject.mesh	Liposomes
dc.subject.mesh	Triple Negative Breast Neoplasms
dc.subject.mesh	Reactive Oxygen Species
dc.subject.mesh	Fluorocarbons
dc.subject.mesh	Hydrocarbons, Brominated
dc.subject.mesh	Protoporphyrins
dc.subject.mesh	Humans
dc.subject.mesh	Reactive Oxygen Species
dc.subject.mesh	Hydrocarbons, Brominated
dc.subject.mesh	Fluorocarbons
dc.subject.mesh	Protoporphyrins
dc.subject.mesh	Liposomes
dc.subject.mesh	Photochemotherapy
dc.subject.mesh	Triple Negative Breast Neoplasms
dc.subject.mesh	Humans
dc.subject.mesh	Photochemotherapy
dc.subject.mesh	Liposomes
dc.subject.mesh	Triple Negative Breast Neoplasms
dc.subject.mesh	Reactive Oxygen Species
dc.subject.mesh	Fluorocarbons
dc.subject.mesh	Hydrocarbons, Brominated
dc.subject.mesh	Protoporphyrins
dc.title	Improved effectiveness of X-PDT against human triple-negative breast cancer cells through the use of liposomes co-loaded with protoporphyrin IX and perfluorooctyl bromide.
dc.type	Journal Article
utslib.citation.volume	12
utslib.location.activity	England
utslib.for	0303 Macromolecular and Materials Chemistry
utslib.for	0903 Biomedical Engineering
pubs.organisational-group	University of Technology Sydney
pubs.organisational-group	University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
utslib.copyright.status	closed_access	*
dc.date.updated	2024-11-04T01:26:12Z
pubs.issue	15
pubs.publication-status	Published online
pubs.volume	12
utslib.citation.issue	15

Abstract:

In this study, we utilized X-ray-induced photodynamic therapy (X-PDT) against triple-negative breast cancer (TNBC) cells. To achieve this, we developed a liposome delivery system that co-loaded protoporphyrin IX (PPIX) and perfluorooctyl bromide (PFOB) in a rational manner. Low-dose X-ray at 2 Gy was employed to activate PPIX for the generation of reactive oxygen species (ROS), and the co-loading of PFOB provided additional oxygen to enhance ROS production. The resulting highly toxic ROS effectively induced cell death in TNBC. In vitro X-PDT effects, including intracellular ROS generation, cell viability, and apoptosis/necrosis assays in TNBC cells, were thoroughly investigated. Our results indicate that the nanocarriers effectively induced X-PDT effects with very low-dose radiation, making it feasible to damage cancer cells. This suggests the potential for the effective utilization of X-PDT in treating hypoxic cancers, including TNBC, with only a fraction of conventional radiotherapy.

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http://hdl.handle.net/10453/181693