Serum-derived protein coronas affect nanoparticle interactions with brain cells.

Morshed, N; Rennie, C; Deng, W; Collins-Praino, L; Care, A

Serum-derived protein coronas affect nanoparticle interactions with brain cells.

Morshed, N

Rennie, C

Deng, W

Collins-Praino, L Care, A

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Publisher:: IOP Publishing Ltd
Publication Type:: Journal Article
Citation:: Nanotechnology, 2024, 35, (49)
Issue Date:: 2024-09-25

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Field	Value	Language
dc.contributor.author	Morshed, N https://orcid.org/0000-0001-6398-616X
dc.contributor.author	Rennie, C https://orcid.org/0000-0003-4533-6627
dc.contributor.author	Deng, W https://orcid.org/0000-0002-9413-0978
dc.contributor.author	Collins-Praino, L
dc.contributor.author	Care, A https://orcid.org/0000-0002-0035-7961
dc.date.accessioned	2024-11-04T01:28:12Z
dc.date.available	2024-09-16
dc.date.available	2024-11-04T01:28:12Z
dc.date.issued	2024-09-25
dc.identifier.citation	Nanotechnology, 2024, 35, (49)
dc.identifier.issn	0957-4484
dc.identifier.issn	1361-6528
dc.identifier.uri	http://hdl.handle.net/10453/181694
dc.description.abstract	Neuronanomedicine is an emerging field bridging the gap between neuromedicine and novel nanotherapeutics. Despite promise, clinical translation of neuronanomedicine remains elusive, possibly due to a dearth of information regarding the effect of the protein corona on these neuronanomedicines. The protein corona, a layer of proteins adsorbed to nanoparticles following exposure to biological fluids, ultimately determines the fate of nanoparticles in biological systems, dictating nanoparticle-cell interactions. To date, few studies have investigated the effect of the protein corona on interactions with brain-derived cells, an important consideration for the development of neuronanomedicines. Here, two polymeric nanoparticles, poly(lactic-co-glycolic acid) (PLGA) and PLGA-polyethylene glycol (PLGA-PEG), were used to obtain serum-derived protein coronas. Protein corona characterization and liquid chromatography mass spectrometry analysis revealed distinct differences in biophysical properties and protein composition. PLGA protein coronas contained high abundance of globins (60%) and apolipoproteins (21%), while PLGA-PEG protein coronas contained fewer globins (42%) and high abundance of protease inhibitors (28%). Corona coated PLGA nanoparticles were readily internalized into microglia and neuronal cells, but not into astrocytes. Internalization of nanoparticles was associated with pro-inflammatory cytokine release and decreased neuronal cell viability, however, viability was rescued in cells treated with corona coated nanoparticles. These results showcase the importance of the protein corona in mediating nanoparticle-cell interactions.
dc.format	Electronic
dc.language	eng
dc.publisher	IOP Publishing Ltd
dc.relation.ispartof	Nanotechnology
dc.relation.isbasedon	10.1088/1361-6528/ad7b40
dc.rights	info:eu-repo/semantics/openAccess
dc.subject.classification	Nanoscience & Nanotechnology
dc.subject.mesh	Protein Corona
dc.subject.mesh	Nanoparticles
dc.subject.mesh	Polyethylene Glycols
dc.subject.mesh	Animals
dc.subject.mesh	Polylactic Acid-Polyglycolic Acid Copolymer
dc.subject.mesh	Brain
dc.subject.mesh	Humans
dc.subject.mesh	Neurons
dc.subject.mesh	Astrocytes
dc.subject.mesh	Lactic Acid
dc.subject.mesh	Microglia
dc.subject.mesh	Polyglycolic Acid
dc.subject.mesh	Brain
dc.subject.mesh	Astrocytes
dc.subject.mesh	Microglia
dc.subject.mesh	Neurons
dc.subject.mesh	Animals
dc.subject.mesh	Humans
dc.subject.mesh	Polyethylene Glycols
dc.subject.mesh	Lactic Acid
dc.subject.mesh	Polyglycolic Acid
dc.subject.mesh	Nanoparticles
dc.subject.mesh	Protein Corona
dc.subject.mesh	Polylactic Acid-Polyglycolic Acid Copolymer
dc.title	Serum-derived protein coronas affect nanoparticle interactions with brain cells.
dc.type	Journal Article
utslib.citation.volume	35
utslib.location.activity	England
pubs.organisational-group	University of Technology Sydney
pubs.organisational-group	University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	University of Technology Sydney/Faculty of Science
pubs.organisational-group	University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
utslib.copyright.status	open_access	*
dc.rights.license	This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0). To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/
dc.date.updated	2024-11-04T01:28:10Z
pubs.issue	49
pubs.publication-status	Published online
pubs.volume	35
utslib.citation.issue	49

Abstract:

Neuronanomedicine is an emerging field bridging the gap between neuromedicine and novel nanotherapeutics. Despite promise, clinical translation of neuronanomedicine remains elusive, possibly due to a dearth of information regarding the effect of the protein corona on these neuronanomedicines. The protein corona, a layer of proteins adsorbed to nanoparticles following exposure to biological fluids, ultimately determines the fate of nanoparticles in biological systems, dictating nanoparticle-cell interactions. To date, few studies have investigated the effect of the protein corona on interactions with brain-derived cells, an important consideration for the development of neuronanomedicines. Here, two polymeric nanoparticles, poly(lactic-co-glycolic acid) (PLGA) and PLGA-polyethylene glycol (PLGA-PEG), were used to obtain serum-derived protein coronas. Protein corona characterization and liquid chromatography mass spectrometry analysis revealed distinct differences in biophysical properties and protein composition. PLGA protein coronas contained high abundance of globins (60%) and apolipoproteins (21%), while PLGA-PEG protein coronas contained fewer globins (42%) and high abundance of protease inhibitors (28%). Corona coated PLGA nanoparticles were readily internalized into microglia and neuronal cells, but not into astrocytes. Internalization of nanoparticles was associated with pro-inflammatory cytokine release and decreased neuronal cell viability, however, viability was rescued in cells treated with corona coated nanoparticles. These results showcase the importance of the protein corona in mediating nanoparticle-cell interactions.

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http://hdl.handle.net/10453/181694