A portable multi-taxa phenotyping device to retrieve physiological performance traits.

England, H; Herdean, A; Matthews, J; Hughes, DJ; Roper, CD; Suggett, DJ; Voolstra, CR; Camp, EF

A portable multi-taxa phenotyping device to retrieve physiological performance traits.

England, H Herdean, A

Matthews, J Hughes, DJ Roper, CD Suggett, DJ Voolstra, CR Camp, EF

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Publisher:: NATURE PORTFOLIO
Publication Type:: Journal Article
Citation:: Sci Rep, 2024, 14, (1), pp. 21826
Issue Date:: 2024-09-18

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Field	Value	Language
dc.contributor.author	England, H
dc.contributor.author	Herdean, A https://orcid.org/0000-0003-2143-0213
dc.contributor.author	Matthews, J
dc.contributor.author	Hughes, DJ
dc.contributor.author	Roper, CD
dc.contributor.author	Suggett, DJ
dc.contributor.author	Voolstra, CR
dc.contributor.author	Camp, EF
dc.date.accessioned	2024-11-21T23:05:59Z
dc.date.available	2024-09-02
dc.date.available	2024-11-21T23:05:59Z
dc.date.issued	2024-09-18
dc.identifier.citation	Sci Rep, 2024, 14, (1), pp. 21826
dc.identifier.issn	2045-2322
dc.identifier.issn	2045-2322
dc.identifier.uri	http://hdl.handle.net/10453/182030
dc.description.abstract	Organismal phenotyping to identify fitness traits is transforming our understanding of adaptive responses and ecological interactions of species within changing environments. Here we present a portable Multi-Taxa Phenotyping (MTP) system that can retrieve a suite of metabolic and photophysiological parameter across light, temperature, and/or chemical gradients, using real time bio-optical (oxygen and chlorophyll a fluorescence) measurements. The MTP system integrates three well-established technologies for the first time: an imaging Pulse Amplitude Modulated (PAM) chlorophyll a fluorometer, custom-designed well plates equipped with optical oxygen sensors, and a thermocycler. We demonstrate the ability of the MTP system to distinguish phenotypic performance characteristics of diverse aquatic taxa spanning corals, mangroves and algae based on metabolic parameters and Photosystem II dynamics, in a high-throughput capacity and accounting for interactions of different environmental gradients on performance. Extracted metrics from the MTP system can not only provide information on the performance of aquatic taxa exposed to differing environmental gradients, but also provide predicted phenotypic responses of key aquatic organisms to environmental change. Further work validating how rapid phenotyping tools such as the MTP system predict phenotypic responses to long term environmental changes in situ are urgently required to best inform how these tools can support management efforts.
dc.format	Electronic
dc.language	eng
dc.publisher	NATURE PORTFOLIO
dc.relation.ispartof	Sci Rep
dc.relation.isbasedon	10.1038/s41598-024-71972-5
dc.rights	info:eu-repo/semantics/openAccess
dc.subject.mesh	Phenotype
dc.subject.mesh	Animals
dc.subject.mesh	Chlorophyll A
dc.subject.mesh	Aquatic Organisms
dc.subject.mesh	Anthozoa
dc.subject.mesh	Oxygen
dc.subject.mesh	Chlorophyll
dc.subject.mesh	Photosystem II Protein Complex
dc.subject.mesh	Animals
dc.subject.mesh	Anthozoa
dc.subject.mesh	Oxygen
dc.subject.mesh	Chlorophyll
dc.subject.mesh	Photosystem II Protein Complex
dc.subject.mesh	Phenotype
dc.subject.mesh	Aquatic Organisms
dc.subject.mesh	Chlorophyll A
dc.subject.mesh	Phenotype
dc.subject.mesh	Animals
dc.subject.mesh	Chlorophyll A
dc.subject.mesh	Aquatic Organisms
dc.subject.mesh	Anthozoa
dc.subject.mesh	Oxygen
dc.subject.mesh	Chlorophyll
dc.subject.mesh	Photosystem II Protein Complex
dc.title	A portable multi-taxa phenotyping device to retrieve physiological performance traits.
dc.type	Journal Article
utslib.citation.volume	14
utslib.location.activity	England
pubs.organisational-group	University of Technology Sydney
pubs.organisational-group	University of Technology Sydney/Faculty of Science
pubs.organisational-group	University of Technology Sydney/UTS Groups
pubs.organisational-group	University of Technology Sydney/UTS Groups/Climate Change Cluster Research Strength (C3)
pubs.organisational-group	University of Technology Sydney/UTS Groups/Climate Change Cluster Research Strength (C3)/Climate Change Cluster Research Strength (C3) Associate Members
utslib.copyright.status	open_access	*
dc.rights.license	This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND 4.0). To view a copy of this license, visit https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.date.updated	2024-11-21T23:05:57Z
pubs.issue	1
pubs.publication-status	Published online
pubs.volume	14
utslib.citation.issue	1

Abstract:

Organismal phenotyping to identify fitness traits is transforming our understanding of adaptive responses and ecological interactions of species within changing environments. Here we present a portable Multi-Taxa Phenotyping (MTP) system that can retrieve a suite of metabolic and photophysiological parameter across light, temperature, and/or chemical gradients, using real time bio-optical (oxygen and chlorophyll a fluorescence) measurements. The MTP system integrates three well-established technologies for the first time: an imaging Pulse Amplitude Modulated (PAM) chlorophyll a fluorometer, custom-designed well plates equipped with optical oxygen sensors, and a thermocycler. We demonstrate the ability of the MTP system to distinguish phenotypic performance characteristics of diverse aquatic taxa spanning corals, mangroves and algae based on metabolic parameters and Photosystem II dynamics, in a high-throughput capacity and accounting for interactions of different environmental gradients on performance. Extracted metrics from the MTP system can not only provide information on the performance of aquatic taxa exposed to differing environmental gradients, but also provide predicted phenotypic responses of key aquatic organisms to environmental change. Further work validating how rapid phenotyping tools such as the MTP system predict phenotypic responses to long term environmental changes in situ are urgently required to best inform how these tools can support management efforts.

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http://hdl.handle.net/10453/182030