Lipidomic changes occurring in platelets during extended cold storage.
- Publisher:
- WILEY
- Publication Type:
- Journal Article
- Citation:
- Transfus Med, 2024, 34, (3), pp. 189-199
- Issue Date:
- 2024-06
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Full metadata record
| Field | Value | Language |
|---|---|---|
| dc.contributor.author | Green, SM | |
| dc.contributor.author | Padula, MP | |
| dc.contributor.author | Dodgen, TM | |
| dc.contributor.author | Batarseh, A | |
| dc.contributor.author | Marks, DC | |
| dc.contributor.author | Johnson, L | |
| dc.date.accessioned | 2024-12-17T00:21:37Z | |
| dc.date.available | 2024-04-13 | |
| dc.date.available | 2024-12-17T00:21:37Z | |
| dc.date.issued | 2024-06 | |
| dc.identifier.citation | Transfus Med, 2024, 34, (3), pp. 189-199 | |
| dc.identifier.issn | 0958-7578 | |
| dc.identifier.issn | 1365-3148 | |
| dc.identifier.uri | http://hdl.handle.net/10453/182609 | |
| dc.description.abstract | OBJECTIVES: Cold storage is being implemented as an alternative to conventional room-temperature storage for extending the shelf-life of platelet components beyond 5-7 days. The aim of this study was to characterise the lipid profile of platelets stored under standard room-temperature or cold (refrigerated) conditions. METHODS: Matched apheresis derived platelet components in 60% PAS-E/40% plasma (n = 8) were stored at room-temperature (20-24°C with agitation) or in the cold (2-6°C without agitation). Platelets were sampled on day 1, 5 and 14. The lipidome was assessed by ultra-pressure liquid chromatography ion mobility quadrupole time of flight mass spectrometry (UPLC IMS QToF). Changes in bioactive lipid mediators were measured by ELISA. RESULTS: The total phospholipid and sphingolipid content of the platelets and supernatant were 44 544 ± 2915 μg/mL and 38 990 ± 10 880 μg/mL, respectively, and was similar over 14 days, regardless of storage temperature. The proportion of the procoagulant lipids, phosphatidylserine (PS) and phosphatidylethanolamine (PE), increased by 2.7% and 12.2%, respectively, during extended cold storage. Cold storage for 14 days increased sphingomyelin (SM) by 4.1% and decreased ceramide by 1.6% compared to day 1. Further, lysophosphatidylcholine (LPC) species remained unchanged during cold storage for 14 days. The concentration of 12- and 15-hydroxyeicosatetraenoic acid (HETE) were lower in the supernatant of cold-stored platelets than room-temperature controls stored for 14 days. CONCLUSION: The lipid profile of platelets was relatively unchanged during storage for 5 days, regardless of temperature. However, during extended cold storage (14 days) the proportion of the procoagulant lipids, PS and PE, increased, while LPC and bioactive lipids were stable. | |
| dc.format | Print-Electronic | |
| dc.language | eng | |
| dc.publisher | WILEY | |
| dc.relation.ispartof | Transfus Med | |
| dc.relation.isbasedon | 10.1111/tme.13043 | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.subject | 1103 Clinical Sciences | |
| dc.subject.classification | Cardiovascular System & Hematology | |
| dc.subject.classification | 3202 Clinical sciences | |
| dc.subject.mesh | Humans | |
| dc.subject.mesh | Blood Preservation | |
| dc.subject.mesh | Blood Platelets | |
| dc.subject.mesh | Lipidomics | |
| dc.subject.mesh | Cold Temperature | |
| dc.subject.mesh | Male | |
| dc.subject.mesh | Female | |
| dc.subject.mesh | Time Factors | |
| dc.subject.mesh | Phospholipids | |
| dc.subject.mesh | Adult | |
| dc.subject.mesh | Sphingolipids | |
| dc.subject.mesh | Blood Platelets | |
| dc.subject.mesh | Humans | |
| dc.subject.mesh | Phospholipids | |
| dc.subject.mesh | Sphingolipids | |
| dc.subject.mesh | Blood Preservation | |
| dc.subject.mesh | Time Factors | |
| dc.subject.mesh | Adult | |
| dc.subject.mesh | Female | |
| dc.subject.mesh | Male | |
| dc.subject.mesh | Cold Temperature | |
| dc.subject.mesh | Lipidomics | |
| dc.subject.mesh | Humans | |
| dc.subject.mesh | Blood Preservation | |
| dc.subject.mesh | Blood Platelets | |
| dc.subject.mesh | Lipidomics | |
| dc.subject.mesh | Cold Temperature | |
| dc.subject.mesh | Male | |
| dc.subject.mesh | Female | |
| dc.subject.mesh | Time Factors | |
| dc.subject.mesh | Phospholipids | |
| dc.subject.mesh | Adult | |
| dc.subject.mesh | Sphingolipids | |
| dc.title | Lipidomic changes occurring in platelets during extended cold storage. | |
| dc.type | Journal Article | |
| utslib.citation.volume | 34 | |
| utslib.location.activity | England | |
| utslib.for | 1103 Clinical Sciences | |
| pubs.organisational-group | University of Technology Sydney | |
| pubs.organisational-group | University of Technology Sydney/Faculty of Science | |
| pubs.organisational-group | University of Technology Sydney/Faculty of Science/School of Life Sciences | |
| pubs.organisational-group | University of Technology Sydney/UTS Groups | |
| pubs.organisational-group | University of Technology Sydney/UTS Groups/Australian Institute for Microbiology & Infection (AIMI) | |
| pubs.organisational-group | University of Technology Sydney/UTS Groups/Australian Institute for Microbiology & Infection (AIMI)/Australian Institute for Microbiology & Infection (AIMI) Associate Members | |
| utslib.copyright.status | open_access | * |
| dc.rights.license | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND 4.0). To view a copy of this license, visit https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
| dc.date.updated | 2024-12-17T00:21:35Z | |
| pubs.issue | 3 | |
| pubs.publication-status | Published | |
| pubs.volume | 34 | |
| utslib.citation.issue | 3 |
Abstract:
OBJECTIVES: Cold storage is being implemented as an alternative to conventional room-temperature storage for extending the shelf-life of platelet components beyond 5-7 days. The aim of this study was to characterise the lipid profile of platelets stored under standard room-temperature or cold (refrigerated) conditions. METHODS: Matched apheresis derived platelet components in 60% PAS-E/40% plasma (n = 8) were stored at room-temperature (20-24°C with agitation) or in the cold (2-6°C without agitation). Platelets were sampled on day 1, 5 and 14. The lipidome was assessed by ultra-pressure liquid chromatography ion mobility quadrupole time of flight mass spectrometry (UPLC IMS QToF). Changes in bioactive lipid mediators were measured by ELISA. RESULTS: The total phospholipid and sphingolipid content of the platelets and supernatant were 44 544 ± 2915 μg/mL and 38 990 ± 10 880 μg/mL, respectively, and was similar over 14 days, regardless of storage temperature. The proportion of the procoagulant lipids, phosphatidylserine (PS) and phosphatidylethanolamine (PE), increased by 2.7% and 12.2%, respectively, during extended cold storage. Cold storage for 14 days increased sphingomyelin (SM) by 4.1% and decreased ceramide by 1.6% compared to day 1. Further, lysophosphatidylcholine (LPC) species remained unchanged during cold storage for 14 days. The concentration of 12- and 15-hydroxyeicosatetraenoic acid (HETE) were lower in the supernatant of cold-stored platelets than room-temperature controls stored for 14 days. CONCLUSION: The lipid profile of platelets was relatively unchanged during storage for 5 days, regardless of temperature. However, during extended cold storage (14 days) the proportion of the procoagulant lipids, PS and PE, increased, while LPC and bioactive lipids were stable.
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