NIR light-controlled DNA nanodevice for amplified mRNA imaging and precise gene therapy

Xiang, M; Jiang, Y; Zhou, J; Bao, G; Luo, X; Zhang, L; Jin, D; Xian, Y; Zhang, C

NIR light-controlled DNA nanodevice for amplified mRNA imaging and precise gene therapy

Xiang, M Jiang, Y Zhou, J

Bao, G

Luo, X Zhang, L Jin, D

Xian, Y Zhang, C

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Publisher:: ELSEVIER SCI LTD
Publication Type:: Journal Article
Citation:: Nano Today, 2024, 54
Issue Date:: 2024-02-01

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Field	Value	Language
dc.contributor.author	Xiang, M
dc.contributor.author	Jiang, Y
dc.contributor.author	Zhou, J https://orcid.org/0000-0002-0605-5745
dc.contributor.author	Bao, G https://orcid.org/0000-0001-5103-5009
dc.contributor.author	Luo, X
dc.contributor.author	Zhang, L
dc.contributor.author	Jin, D https://orcid.org/0000-0003-1046-2666
dc.contributor.author	Xian, Y
dc.contributor.author	Zhang, C
dc.date.accessioned	2024-12-17T01:33:03Z
dc.date.available	2024-12-17T01:33:03Z
dc.date.issued	2024-02-01
dc.identifier.citation	Nano Today, 2024, 54
dc.identifier.issn	1748-0132
dc.identifier.issn	1878-044X
dc.identifier.uri	http://hdl.handle.net/10453/182640
dc.description.abstract	Accurately delivering antisense oligonucleotides (ASOs) to tumor cells for gene therapy poses a significant challenge. To address this issue, we develop a NIR light-activable DNA nanodevice to target survivin mRNA and simultaneously release ASOs in tumor cells, which allows high-precision spatiotemporal imaging and efficient gene therapy. The concept is based on upconversion nanoparticles, which can convert NIR light into UV emissions to activate an entropy-driven DNA walking system for mRNA recognition in tumor cells. The intramolecular toehold-mediated entropy-driven catalytic reaction generates a large amount of Bcl-2 ASOs, which allows accurate delivery of ASOs for gene therapy due to the specific targeting of mRNA in tumor cells. The results from in vitro and in vivo experiments show that the NIR light-activated DNA nanodevice can detect mRNA with high sensitivity by DNA walking amplification and high precision in gene delivery. Moreover, the released ASOs from DNA walking system down-regulate the expression of Bcl-2 anti-apoptosis protein and induce tumor cell apoptosis, thereby suppressing tumor growth without a transfection reagent. The NIR-light-controlled DNA nanodevice holds great potential for precise gene therapy in clinical applications.
dc.language	English
dc.publisher	ELSEVIER SCI LTD
dc.relation	University of Technology Sydney
dc.relation.ispartof	Nano Today
dc.relation.isbasedon	10.1016/j.nantod.2023.102110
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0903 Biomedical Engineering, 1007 Nanotechnology
dc.subject.classification	Nanoscience & Nanotechnology
dc.subject.classification	3206 Medical biotechnology
dc.subject.classification	4003 Biomedical engineering
dc.subject.classification	4018 Nanotechnology
dc.title	NIR light-controlled DNA nanodevice for amplified mRNA imaging and precise gene therapy
dc.type	Journal Article
utslib.citation.volume	54
utslib.for	0903 Biomedical Engineering
utslib.for	1007 Nanotechnology
pubs.organisational-group	University of Technology Sydney
pubs.organisational-group	University of Technology Sydney/Faculty of Science
pubs.organisational-group	University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	University of Technology Sydney/UTS Groups
pubs.organisational-group	University of Technology Sydney/UTS Groups/Institute of Biomedical Materials and Devices (IBMD)
utslib.copyright.status	closed_access	*
dc.date.updated	2024-12-17T01:33:01Z
pubs.publication-status	Published
pubs.volume	54

Abstract:

Accurately delivering antisense oligonucleotides (ASOs) to tumor cells for gene therapy poses a significant challenge. To address this issue, we develop a NIR light-activable DNA nanodevice to target survivin mRNA and simultaneously release ASOs in tumor cells, which allows high-precision spatiotemporal imaging and efficient gene therapy. The concept is based on upconversion nanoparticles, which can convert NIR light into UV emissions to activate an entropy-driven DNA walking system for mRNA recognition in tumor cells. The intramolecular toehold-mediated entropy-driven catalytic reaction generates a large amount of Bcl-2 ASOs, which allows accurate delivery of ASOs for gene therapy due to the specific targeting of mRNA in tumor cells. The results from in vitro and in vivo experiments show that the NIR light-activated DNA nanodevice can detect mRNA with high sensitivity by DNA walking amplification and high precision in gene delivery. Moreover, the released ASOs from DNA walking system down-regulate the expression of Bcl-2 anti-apoptosis protein and induce tumor cell apoptosis, thereby suppressing tumor growth without a transfection reagent. The NIR-light-controlled DNA nanodevice holds great potential for precise gene therapy in clinical applications.

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http://hdl.handle.net/10453/182640