Distinct Effects of Respiratory Viral Infection Models on miR-149-5p, IL-6 and p63 Expression in BEAS-2B and A549 Epithelial Cells.
- Publisher:
- MDPI
- Publication Type:
- Journal Article
- Citation:
- Cells, 2024, 13, (11), pp. 919
- Issue Date:
- 2024-05-26
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Full metadata record
| Field | Value | Language |
|---|---|---|
| dc.contributor.author | Shahdab, N | |
| dc.contributor.author | Ward, C | |
| dc.contributor.author | Hansbro, PM | |
| dc.contributor.author | Cummings, S | |
| dc.contributor.author | Young, JS | |
| dc.contributor.author | Moheimani, F | |
| dc.date.accessioned | 2025-01-02T04:10:05Z | |
| dc.date.available | 2024-05-21 | |
| dc.date.available | 2025-01-02T04:10:05Z | |
| dc.date.issued | 2024-05-26 | |
| dc.identifier.citation | Cells, 2024, 13, (11), pp. 919 | |
| dc.identifier.issn | 2073-4409 | |
| dc.identifier.issn | 2073-4409 | |
| dc.identifier.uri | http://hdl.handle.net/10453/182820 | |
| dc.description.abstract | Respiratory viruses cause airway inflammation, resulting in epithelial injury and repair. miRNAs, including miR-149-5p, regulate different pathological conditions. We aimed to determine how miR-149-5p functions in regulating pro-inflammatory IL-6 and p63, key regulators of airway epithelial wound repair, in response to viral proteins in bronchial (BEAS-2B) and alveolar (A549) epithelial cells. BEAS-2B or A549 cells were incubated with poly (I:C, 0.5 µg/mL) for 48 h or SARS-CoV-2 spike protein-1 or 2 subunit (S1 or S2, 1 μg/mL) for 24 h. miR-149-5p was suppressed in BEAS-2B challenged with poly (I:C), correlating with IL-6 and p63 upregulation. miR-149-5p was down-regulated in A549 stimulated with poly (I:C); IL-6 expression increased, but p63 protein levels were undetectable. miR-149-5p remained unchanged in cells exposed to S1 or S2, while S1 transfection increased IL-6 expression in BEAS-2B cells. Ectopic over-expression of miR-149-5p in BEAS-2B cells suppressed IL-6 and p63 mRNA levels and inhibited poly (I:C)-induced IL-6 and p63 mRNA expressions. miR-149-5p directly suppressed IL-6 mRNA in BEAS-2B cells. Hence, BEAS-2B cells respond differently to poly (I:C), S1 or S2 compared to A549 cells. Thus, miR-149-5p dysregulation may be involved in poly (I:C)-stimulated but not S1- or S2-stimulated increased IL-6 production and p63 expression in BEAS-2B cells. | |
| dc.format | Electronic | |
| dc.language | eng | |
| dc.publisher | MDPI | |
| dc.relation | http://purl.org/au-research/grants/nhmrc/1175134 | |
| dc.relation | http://purl.org/au-research/grants/nhmrc/2010287 | |
| dc.relation.ispartof | Cells | |
| dc.relation.isbasedon | 10.3390/cells13110919 | |
| dc.rights | info:eu-repo/semantics/openAccess | |
| dc.subject.classification | 31 Biological sciences | |
| dc.subject.classification | 32 Biomedical and clinical sciences | |
| dc.subject.mesh | Humans | |
| dc.subject.mesh | MicroRNAs | |
| dc.subject.mesh | Interleukin-6 | |
| dc.subject.mesh | A549 Cells | |
| dc.subject.mesh | Epithelial Cells | |
| dc.subject.mesh | Poly I-C | |
| dc.subject.mesh | SARS-CoV-2 | |
| dc.subject.mesh | COVID-19 | |
| dc.subject.mesh | Tumor Suppressor Proteins | |
| dc.subject.mesh | Transcription Factors | |
| dc.subject.mesh | Gene Expression Regulation | |
| dc.subject.mesh | Epithelial Cells | |
| dc.subject.mesh | Humans | |
| dc.subject.mesh | Tumor Suppressor Proteins | |
| dc.subject.mesh | Transcription Factors | |
| dc.subject.mesh | MicroRNAs | |
| dc.subject.mesh | Poly I-C | |
| dc.subject.mesh | Interleukin-6 | |
| dc.subject.mesh | Gene Expression Regulation | |
| dc.subject.mesh | A549 Cells | |
| dc.subject.mesh | COVID-19 | |
| dc.subject.mesh | SARS-CoV-2 | |
| dc.subject.mesh | Humans | |
| dc.subject.mesh | MicroRNAs | |
| dc.subject.mesh | Interleukin-6 | |
| dc.subject.mesh | A549 Cells | |
| dc.subject.mesh | Epithelial Cells | |
| dc.subject.mesh | Poly I-C | |
| dc.subject.mesh | SARS-CoV-2 | |
| dc.subject.mesh | COVID-19 | |
| dc.subject.mesh | Tumor Suppressor Proteins | |
| dc.subject.mesh | Transcription Factors | |
| dc.subject.mesh | Gene Expression Regulation | |
| dc.title | Distinct Effects of Respiratory Viral Infection Models on miR-149-5p, IL-6 and p63 Expression in BEAS-2B and A549 Epithelial Cells. | |
| dc.type | Journal Article | |
| utslib.citation.volume | 13 | |
| utslib.location.activity | Switzerland | |
| pubs.organisational-group | University of Technology Sydney | |
| pubs.organisational-group | University of Technology Sydney/Faculty of Science | |
| pubs.organisational-group | University of Technology Sydney/Faculty of Science/School of Life Sciences | |
| pubs.organisational-group | University of Technology Sydney/UTS Groups | |
| pubs.organisational-group | University of Technology Sydney/UTS Groups/Australian Institute for Microbiology & Infection (AIMI) | |
| pubs.organisational-group | University of Technology Sydney/UTS Groups/Centre for Inflammation (CFI) | |
| pubs.organisational-group | University of Technology Sydney/UTS Groups/Australian Institute for Microbiology & Infection (AIMI)/Australian Institute for Microbiology & Infection (AIMI) Associate Members | |
| utslib.copyright.status | open_access | * |
| dc.rights.license | This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0). To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/ | |
| dc.date.updated | 2025-01-02T04:10:02Z | |
| pubs.issue | 11 | |
| pubs.publication-status | Published online | |
| pubs.volume | 13 | |
| utslib.citation.issue | 11 |
Abstract:
Respiratory viruses cause airway inflammation, resulting in epithelial injury and repair. miRNAs, including miR-149-5p, regulate different pathological conditions. We aimed to determine how miR-149-5p functions in regulating pro-inflammatory IL-6 and p63, key regulators of airway epithelial wound repair, in response to viral proteins in bronchial (BEAS-2B) and alveolar (A549) epithelial cells. BEAS-2B or A549 cells were incubated with poly (I:C, 0.5 µg/mL) for 48 h or SARS-CoV-2 spike protein-1 or 2 subunit (S1 or S2, 1 μg/mL) for 24 h. miR-149-5p was suppressed in BEAS-2B challenged with poly (I:C), correlating with IL-6 and p63 upregulation. miR-149-5p was down-regulated in A549 stimulated with poly (I:C); IL-6 expression increased, but p63 protein levels were undetectable. miR-149-5p remained unchanged in cells exposed to S1 or S2, while S1 transfection increased IL-6 expression in BEAS-2B cells. Ectopic over-expression of miR-149-5p in BEAS-2B cells suppressed IL-6 and p63 mRNA levels and inhibited poly (I:C)-induced IL-6 and p63 mRNA expressions. miR-149-5p directly suppressed IL-6 mRNA in BEAS-2B cells. Hence, BEAS-2B cells respond differently to poly (I:C), S1 or S2 compared to A549 cells. Thus, miR-149-5p dysregulation may be involved in poly (I:C)-stimulated but not S1- or S2-stimulated increased IL-6 production and p63 expression in BEAS-2B cells.
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