Microfluidic SERS biosensor based on Au-semicoated photonic crystals for melanoma diagnosis.

Wang, W; Mao, W; Sun, H; Hou, F; Wang, W; Liu, W; Shi, Z; Lin, G; Wang, M; Fang, G; Cheng, YY; Xu, C

Microfluidic SERS biosensor based on Au-semicoated photonic crystals for melanoma diagnosis.

Wang, W Mao, W Sun, H Hou, F Wang, W Liu, W Shi, Z Lin, G

Wang, M Fang, G Cheng, YY Xu, C

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Publisher:: Elsevier
Publication Type:: Journal Article
Citation:: Biosens Bioelectron, 2025, 271, pp. 116983
Issue Date:: 2025-03-01

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Field	Value	Language
dc.contributor.author	Wang, W
dc.contributor.author	Mao, W
dc.contributor.author	Sun, H
dc.contributor.author	Hou, F
dc.contributor.author	Wang, W
dc.contributor.author	Liu, W
dc.contributor.author	Shi, Z
dc.contributor.author	Lin, G https://orcid.org/0000-0001-9880-8478
dc.contributor.author	Wang, M
dc.contributor.author	Fang, G
dc.contributor.author	Cheng, YY
dc.contributor.author	Xu, C
dc.date.accessioned	2025-01-21T06:09:14Z
dc.date.available	2024-11-20
dc.date.available	2025-01-21T06:09:14Z
dc.date.issued	2025-03-01
dc.identifier.citation	Biosens Bioelectron, 2025, 271, pp. 116983
dc.identifier.issn	0956-5663
dc.identifier.issn	1873-4235
dc.identifier.uri	http://hdl.handle.net/10453/183959
dc.description.abstract	Surface-enhanced Raman scattering (SERS) shows great promise for early diagnosis due to its high specificity and rapid detection capabilities. However, its application is often hindered by substrate instability and insufficient interaction between the substrate and incident light. To address these challenges, a photonic-plasmonic strategy is often employed to enhance sensing performance but it is generally limited by the low efficiency of plasmonic metal and optical cavity resonances. In this study, we significantly improved resonance efficiency by optimizing the photonic crystal configuration and designing Au-semicoated polystyrene nanospheres. These modifications maximized light capture and resonance efficiency, resulting in a 790-fold enhancement of the Raman signal with a relative standard deviation of only 4.58%. This approach was further developed into microfluidic biosensors for melanoma diagnosis, achieving a 2-3 order-of-magnitude improvement over comparable SERS biosensors. We believe this technology has the potential to significantly improve the efficiency of early diagnosis and clinical medical analysis.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	Elsevier
dc.relation.ispartof	Biosens Bioelectron
dc.relation.isbasedon	10.1016/j.bios.2024.116983
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	0301 Analytical Chemistry, 0903 Biomedical Engineering, 1007 Nanotechnology
dc.subject.classification	Bioinformatics
dc.subject.classification	3401 Analytical chemistry
dc.subject.classification	4003 Biomedical engineering
dc.subject.classification	4018 Nanotechnology
dc.subject.mesh	Gold
dc.subject.mesh	Spectrum Analysis, Raman
dc.subject.mesh	Humans
dc.subject.mesh	Biosensing Techniques
dc.subject.mesh	Melanoma
dc.subject.mesh	Photons
dc.subject.mesh	Polystyrenes
dc.subject.mesh	Nanospheres
dc.subject.mesh	Metal Nanoparticles
dc.subject.mesh	Equipment Design
dc.subject.mesh	Microfluidic Analytical Techniques
dc.title	Microfluidic SERS biosensor based on Au-semicoated photonic crystals for melanoma diagnosis.
dc.type	Journal Article
utslib.citation.volume	271
utslib.location.activity	England
utslib.for	0301 Analytical Chemistry
utslib.for	0903 Biomedical Engineering
utslib.for	1007 Nanotechnology
pubs.organisational-group	University of Technology Sydney
pubs.organisational-group	University of Technology Sydney/Faculty of Science
pubs.organisational-group	University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	University of Technology Sydney/UTS Groups
pubs.organisational-group	University of Technology Sydney/UTS Groups/Institute of Biomedical Materials and Devices (IBMD)
utslib.copyright.status	open_access	*
dc.rights.license	This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0). To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/
dc.date.updated	2025-01-21T06:09:11Z
pubs.publication-status	Published
pubs.volume	271

Abstract:

Surface-enhanced Raman scattering (SERS) shows great promise for early diagnosis due to its high specificity and rapid detection capabilities. However, its application is often hindered by substrate instability and insufficient interaction between the substrate and incident light. To address these challenges, a photonic-plasmonic strategy is often employed to enhance sensing performance but it is generally limited by the low efficiency of plasmonic metal and optical cavity resonances. In this study, we significantly improved resonance efficiency by optimizing the photonic crystal configuration and designing Au-semicoated polystyrene nanospheres. These modifications maximized light capture and resonance efficiency, resulting in a 790-fold enhancement of the Raman signal with a relative standard deviation of only 4.58%. This approach was further developed into microfluidic biosensors for melanoma diagnosis, achieving a 2-3 order-of-magnitude improvement over comparable SERS biosensors. We believe this technology has the potential to significantly improve the efficiency of early diagnosis and clinical medical analysis.

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http://hdl.handle.net/10453/183959