Optimization of Flavonoid Extraction from Eucommia ulmoides pollen using Respond Surface Methodology and its biological activities.

Zhu, J; Chen, A; Ma, H; Cheng, YY; Song, K

Optimization of Flavonoid Extraction from Eucommia ulmoides pollen using Respond Surface Methodology and its biological activities.

Zhu, J Chen, A Ma, H Cheng, YY Song, K

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Publisher:: Wiley
Publication Type:: Journal Article
Citation:: Chem Biodivers, 2024, 21, (2), pp. e202301308
Issue Date:: 2024-02

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Field	Value	Language
dc.contributor.author	Zhu, J
dc.contributor.author	Chen, A
dc.contributor.author	Ma, H
dc.contributor.author	Cheng, YY
dc.contributor.author	Song, K
dc.date.accessioned	2025-01-21T06:27:57Z
dc.date.available	2023-12-28
dc.date.available	2025-01-21T06:27:57Z
dc.date.issued	2024-02
dc.identifier.citation	Chem Biodivers, 2024, 21, (2), pp. e202301308
dc.identifier.issn	1612-1872
dc.identifier.issn	1612-1880
dc.identifier.uri	http://hdl.handle.net/10453/183968
dc.description.abstract	Flavonoids, known for their abundance in Eucommia ulmoides pollen, possess diverse biological functions, including antioxidants, antibacterial agents, and anti-tumor properties. This study aims to establish effective parameters for flavonoid extraction from Eucommia ulmoides pollen using a microwave-assisted method, characterize the flavonoid composition of the extracted material, and explore its biological activities. Building upon the initial results from single-factor experiments, response surface methodology was employed to optimize the extraction parameters. The inhibitory effect of human breast cancer cells (MCF-7) was evaluated by CCK assay and Live/dead staining. Simultaneously, the extract's scavenging ability against DPPH free radicals and its antibacterial properties against Escherichia coli and Staphylococcus aureus were investigated. The results demonstrated that the flavonoid yield reached 3.28 g per 100 g of pollen, closely aligning with the predicted value. The IC50 for flavonoid-mediated DPPH radical scavenging was 0.04 mg/mL. The extract exhibited a robust inhibitory effect on both Escherichia coli and Staphylococcus aureus. Concurrently, the extract displayed a significant inhibitory effect on the growth and proliferation of MCF-7 cells in a dose-dependent and time-dependent manner. In addition, six kinds of flavonoids have been identified by UPLC-TOF-MS/MS technology, providing further support to the study on the anti-oxidation and anti-tumor mechanism of Eucommia ulmoides pollen extracts.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	Wiley
dc.relation.ispartof	Chem Biodivers
dc.relation.isbasedon	10.1002/cbdv.202301308
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0304 Medicinal and Biomolecular Chemistry, 0305 Organic Chemistry
dc.subject.classification	Medicinal & Biomolecular Chemistry
dc.subject.classification	3404 Medicinal and biomolecular chemistry
dc.subject.classification	3405 Organic chemistry
dc.subject.mesh	Humans
dc.subject.mesh	Eucommiaceae
dc.subject.mesh	Flavonoids
dc.subject.mesh	Tandem Mass Spectrometry
dc.subject.mesh	Antioxidants
dc.subject.mesh	Anti-Bacterial Agents
dc.subject.mesh	Plant Extracts
dc.subject.mesh	Escherichia coli
dc.subject.mesh	Humans
dc.subject.mesh	Escherichia coli
dc.subject.mesh	Eucommiaceae
dc.subject.mesh	Flavonoids
dc.subject.mesh	Plant Extracts
dc.subject.mesh	Antioxidants
dc.subject.mesh	Anti-Bacterial Agents
dc.subject.mesh	Tandem Mass Spectrometry
dc.subject.mesh	Humans
dc.subject.mesh	Eucommiaceae
dc.subject.mesh	Flavonoids
dc.subject.mesh	Tandem Mass Spectrometry
dc.subject.mesh	Antioxidants
dc.subject.mesh	Anti-Bacterial Agents
dc.subject.mesh	Plant Extracts
dc.subject.mesh	Escherichia coli
dc.title	Optimization of Flavonoid Extraction from Eucommia ulmoides pollen using Respond Surface Methodology and its biological activities.
dc.type	Journal Article
utslib.citation.volume	21
utslib.location.activity	Switzerland
utslib.for	0304 Medicinal and Biomolecular Chemistry
utslib.for	0305 Organic Chemistry
pubs.organisational-group	University of Technology Sydney
pubs.organisational-group	University of Technology Sydney/Faculty of Science
pubs.organisational-group	University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	University of Technology Sydney/UTS Groups
pubs.organisational-group	University of Technology Sydney/UTS Groups/Institute of Biomedical Materials and Devices (IBMD)
utslib.copyright.status	closed_access	*
dc.date.updated	2025-01-21T06:27:55Z
pubs.issue	2
pubs.publication-status	Published
pubs.volume	21
utslib.citation.issue	2

Abstract:

Flavonoids, known for their abundance in Eucommia ulmoides pollen, possess diverse biological functions, including antioxidants, antibacterial agents, and anti-tumor properties. This study aims to establish effective parameters for flavonoid extraction from Eucommia ulmoides pollen using a microwave-assisted method, characterize the flavonoid composition of the extracted material, and explore its biological activities. Building upon the initial results from single-factor experiments, response surface methodology was employed to optimize the extraction parameters. The inhibitory effect of human breast cancer cells (MCF-7) was evaluated by CCK assay and Live/dead staining. Simultaneously, the extract's scavenging ability against DPPH free radicals and its antibacterial properties against Escherichia coli and Staphylococcus aureus were investigated. The results demonstrated that the flavonoid yield reached 3.28 g per 100 g of pollen, closely aligning with the predicted value. The IC50 for flavonoid-mediated DPPH radical scavenging was 0.04 mg/mL. The extract exhibited a robust inhibitory effect on both Escherichia coli and Staphylococcus aureus. Concurrently, the extract displayed a significant inhibitory effect on the growth and proliferation of MCF-7 cells in a dose-dependent and time-dependent manner. In addition, six kinds of flavonoids have been identified by UPLC-TOF-MS/MS technology, providing further support to the study on the anti-oxidation and anti-tumor mechanism of Eucommia ulmoides pollen extracts.

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http://hdl.handle.net/10453/183968