Silk fibroin increases the elasticity of alginate-gelatin hydrogels and regulates cardiac cell contractile function in cardiac bioinks.

Vettori, L; Tran, HA; Mahmodi, H; Filipe, EC; Wyllie, K; Liu Chung Ming, C; Cox, TR; Tipper, J; Kabakova, IV; Rnjak-Kovacina, J; Gentile, C

Silk fibroin increases the elasticity of alginate-gelatin hydrogels and regulates cardiac cell contractile function in cardiac bioinks.

Vettori, L Tran, HA Mahmodi, H Filipe, EC Wyllie, K Liu Chung Ming, C Cox, TR Tipper, J

Kabakova, IV Rnjak-Kovacina, J Gentile, C

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Publisher:: IOP Publishing Ltd
Publication Type:: Journal Article
Citation:: Biofabrication, 2024, 16, (3)
Issue Date:: 2024-06-04

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Field	Value	Language
dc.contributor.author	Vettori, L
dc.contributor.author	Tran, HA
dc.contributor.author	Mahmodi, H
dc.contributor.author	Filipe, EC
dc.contributor.author	Wyllie, K
dc.contributor.author	Liu Chung Ming, C
dc.contributor.author	Cox, TR
dc.contributor.author	Tipper, J https://orcid.org/0000-0002-8719-0323
dc.contributor.author	Kabakova, IV
dc.contributor.author	Rnjak-Kovacina, J
dc.contributor.author	Gentile, C https://orcid.org/0000-0002-3689-4275
dc.date.accessioned	2025-01-23T00:35:36Z
dc.date.available	2024-05-22
dc.date.available	2025-01-23T00:35:36Z
dc.date.issued	2024-06-04
dc.identifier.citation	Biofabrication, 2024, 16, (3)
dc.identifier.issn	1758-5082
dc.identifier.issn	1758-5090
dc.identifier.uri	http://hdl.handle.net/10453/184035
dc.description.abstract	Silk fibroin (SF) is a natural protein extracted fromBombyx morisilkworm thread. From its common use in the textile industry, it emerged as a biomaterial with promising biochemical and mechanical properties for applications in the field of tissue engineering and regenerative medicine. In this study, we evaluate for the first time the effects of SF on cardiac bioink formulations containing cardiac spheroids (CSs). First, we evaluate if the SF addition plays a role in the structural and elastic properties of hydrogels containing alginate (Alg) and gelatin (Gel). Then, we test the printability and durability of bioprinted SF-containing hydrogels. Finally, we evaluate whether the addition of SF controls cell viability and function of CSs in Alg-Gel hydrogels. Our findings show that the addition of 1% (w/v) SF to Alg-Gel hydrogels makes them more elastic without affecting cell viability. However, fractional shortening (FS%) of CSs in SF-Alg-Gel hydrogels increases without affecting their contraction frequency, suggesting an improvement in contractile function in the 3D cultures. Altogether, our findings support a promising pathway to bioengineer bioinks containing SF for cardiac applications, with the ability to control mechanical and cellular features in cardiac bioinks.
dc.format	Electronic
dc.language	eng
dc.publisher	IOP Publishing Ltd
dc.relation.ispartof	Biofabrication
dc.relation.isbasedon	10.1088/1758-5090/ad4f1b
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	0903 Biomedical Engineering, 1004 Medical Biotechnology, 1099 Other Technology
dc.subject.classification	3206 Medical biotechnology
dc.subject.classification	4003 Biomedical engineering
dc.subject.mesh	Alginates
dc.subject.mesh	Fibroins
dc.subject.mesh	Gelatin
dc.subject.mesh	Hydrogels
dc.subject.mesh	Animals
dc.subject.mesh	Elasticity
dc.subject.mesh	Myocytes, Cardiac
dc.subject.mesh	Bioprinting
dc.subject.mesh	Cell Survival
dc.subject.mesh	Tissue Engineering
dc.subject.mesh	Ink
dc.subject.mesh	Spheroids, Cellular
dc.subject.mesh	Rats
dc.subject.mesh	Myocardial Contraction
dc.subject.mesh	Spheroids, Cellular
dc.subject.mesh	Myocytes, Cardiac
dc.subject.mesh	Animals
dc.subject.mesh	Rats
dc.subject.mesh	Alginates
dc.subject.mesh	Gelatin
dc.subject.mesh	Fibroins
dc.subject.mesh	Hydrogels
dc.subject.mesh	Tissue Engineering
dc.subject.mesh	Cell Survival
dc.subject.mesh	Myocardial Contraction
dc.subject.mesh	Elasticity
dc.subject.mesh	Ink
dc.subject.mesh	Bioprinting
dc.subject.mesh	Alginates
dc.subject.mesh	Fibroins
dc.subject.mesh	Gelatin
dc.subject.mesh	Hydrogels
dc.subject.mesh	Animals
dc.subject.mesh	Elasticity
dc.subject.mesh	Myocytes, Cardiac
dc.subject.mesh	Bioprinting
dc.subject.mesh	Cell Survival
dc.subject.mesh	Tissue Engineering
dc.subject.mesh	Ink
dc.subject.mesh	Spheroids, Cellular
dc.subject.mesh	Rats
dc.subject.mesh	Myocardial Contraction
dc.title	Silk fibroin increases the elasticity of alginate-gelatin hydrogels and regulates cardiac cell contractile function in cardiac bioinks.
dc.type	Journal Article
utslib.citation.volume	16
utslib.location.activity	England
utslib.for	0903 Biomedical Engineering
utslib.for	1004 Medical Biotechnology
utslib.for	1099 Other Technology
pubs.organisational-group	University of Technology Sydney
pubs.organisational-group	University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	University of Technology Sydney/Faculty of Science
pubs.organisational-group	University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	University of Technology Sydney/UTS Groups
pubs.organisational-group	University of Technology Sydney/UTS Groups/Centre for Health Technologies (CHT)
pubs.organisational-group	University of Technology Sydney/UTS Groups/Institute of Biomedical Materials and Devices (IBMD)
pubs.organisational-group	University of Technology Sydney/UTS Groups/Institute of Biomedical Materials and Devices (IBMD)/Institute of Biomedical Materials and Devices (IBMD) Associate Members
utslib.copyright.status	open_access	*
dc.rights.license	This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0). To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/
dc.date.updated	2025-01-23T00:35:34Z
pubs.issue	3
pubs.publication-status	Published online
pubs.volume	16
utslib.citation.issue	3

Abstract:

Silk fibroin (SF) is a natural protein extracted fromBombyx morisilkworm thread. From its common use in the textile industry, it emerged as a biomaterial with promising biochemical and mechanical properties for applications in the field of tissue engineering and regenerative medicine. In this study, we evaluate for the first time the effects of SF on cardiac bioink formulations containing cardiac spheroids (CSs). First, we evaluate if the SF addition plays a role in the structural and elastic properties of hydrogels containing alginate (Alg) and gelatin (Gel). Then, we test the printability and durability of bioprinted SF-containing hydrogels. Finally, we evaluate whether the addition of SF controls cell viability and function of CSs in Alg-Gel hydrogels. Our findings show that the addition of 1% (w/v) SF to Alg-Gel hydrogels makes them more elastic without affecting cell viability. However, fractional shortening (FS%) of CSs in SF-Alg-Gel hydrogels increases without affecting their contraction frequency, suggesting an improvement in contractile function in the 3D cultures. Altogether, our findings support a promising pathway to bioengineer bioinks containing SF for cardiac applications, with the ability to control mechanical and cellular features in cardiac bioinks.

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http://hdl.handle.net/10453/184035