Nigella sativa Oil-loaded Ethanolic Vesicular Gel for Imiquimod-induced Plaque Psoriasis: Physicochemical Characterization, Rheological Studies, and In vivo Efficacy.

Vihal, S; Pundir, S; Rathore, C; Ranjan Lal, U; Gupta, G; Kumar Singh, S; Dua, K; Kumar Chellappan, D; Negi, P

Nigella sativa Oil-loaded Ethanolic Vesicular Gel for Imiquimod-induced Plaque Psoriasis: Physicochemical Characterization, Rheological Studies, and In vivo Efficacy.

Vihal, S Pundir, S Rathore, C Ranjan Lal, U Gupta, G Kumar Singh, S Dua, K

Kumar Chellappan, D Negi, P

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Publisher:: Bentham Science Publishers
Publication Type:: Journal Article
Citation:: Curr Drug Deliv, 2025, 22, (1), pp. 80-91
Issue Date:: 2025

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Field	Value	Language
dc.contributor.author	Vihal, S
dc.contributor.author	Pundir, S
dc.contributor.author	Rathore, C
dc.contributor.author	Ranjan Lal, U
dc.contributor.author	Gupta, G
dc.contributor.author	Kumar Singh, S
dc.contributor.author	Dua, K https://orcid.org/0000-0002-7507-1159
dc.contributor.author	Kumar Chellappan, D
dc.contributor.author	Negi, P
dc.date.accessioned	2025-01-28T23:28:13Z
dc.date.available	2023-08-18
dc.date.available	2025-01-28T23:28:13Z
dc.date.issued	2025
dc.identifier.citation	Curr Drug Deliv, 2025, 22, (1), pp. 80-91
dc.identifier.issn	1567-2018
dc.identifier.issn	1875-5704
dc.identifier.uri	http://hdl.handle.net/10453/184555
dc.description.abstract	BACKGROUND: The therapeutic effect of NS oil in mild to moderate psoriasis is limited owing to low play load of thymoquinone (<15 %w/w), irritation, dripping, low viscosity and thus, less contact time on the lesions. AIMS: This study aimed at developing and characterizing the ethanolic vesicular hydrogel system of Nigella sativa (NS) oil (NS EV hydrogel) for the enhancement of anti-psoriatic activity. OBJECTIVE: The objective of this study was to develop NS EV hydrogel and evaluate its anti-psoriatic activity. METHODS: The identification and quantification of TQ content in different NS seed extracts and marketed oil were measured by an HPTLC method using n-hexane and ethyl acetate as solvent systems. Preparation of ethanolic vesicles (EVs) was performed by solvent injection method, while its antipsoriatic activity was evaluated employing an Imiquad (IMQ)-induced plaque psoriasis animal model. RESULTS: A compact HPTLC band was obtained for TQ at an Rf value of 0.651. The calibration plot was linear in the range of 1-10 μg/spot, and the correlation coefficient of 0.990 was indicative of good linear dependence of peak area on concentration. From the different NS sources, the high TQ content was obtained in the marketed cold press oil, i.e., 1.45±0.08 mg/ml. Out of various NS oilloaded EVs, the F6 formulation revealed the smallest particle size (278.1 nm), with log-normal size distribution (0.459) and adequate entrapment efficiency. A non-uniform shape was observed in the transmission electron microscopy. The viscosity of F6 formulation hydrogel was 32.34 (Pa·s), which exhibited plastic behavior. In vivo, efficacy studies demonstrated decreased inflammation of the epidermis and dermis and a marked decrease in the levels of IL-17 by NS EV hydrogel compared to plain NS oil and standard drugs (Betamethasone and Dr. JRK Psorolin Oil). CONCLUSION: It may be concluded from the findings that NS-loaded EV gel was as good as betamethasone cream but more efficacious than the other treatments.
dc.format	Print
dc.language	eng
dc.publisher	Bentham Science Publishers
dc.relation.ispartof	Curr Drug Deliv
dc.relation.isbasedon	10.2174/0115672018246645231019131748
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject	1115 Pharmacology and Pharmaceutical Sciences
dc.subject.classification	Pharmacology & Pharmacy
dc.subject.classification	3214 Pharmacology and pharmaceutical sciences
dc.subject.mesh	Psoriasis
dc.subject.mesh	Animals
dc.subject.mesh	Plant Oils
dc.subject.mesh	Rheology
dc.subject.mesh	Imiquimod
dc.subject.mesh	Ethanol
dc.subject.mesh	Male
dc.subject.mesh	Nigella sativa
dc.subject.mesh	Hydrogels
dc.subject.mesh	Mice
dc.subject.mesh	Gels
dc.subject.mesh	Carum
dc.subject.mesh	Animals
dc.subject.mesh	Mice
dc.subject.mesh	Carum
dc.subject.mesh	Nigella sativa
dc.subject.mesh	Psoriasis
dc.subject.mesh	Ethanol
dc.subject.mesh	Plant Oils
dc.subject.mesh	Gels
dc.subject.mesh	Hydrogels
dc.subject.mesh	Rheology
dc.subject.mesh	Male
dc.subject.mesh	Imiquimod
dc.subject.mesh	Psoriasis
dc.subject.mesh	Animals
dc.subject.mesh	Plant Oils
dc.subject.mesh	Rheology
dc.subject.mesh	Imiquimod
dc.subject.mesh	Ethanol
dc.subject.mesh	Male
dc.subject.mesh	Nigella sativa
dc.subject.mesh	Hydrogels
dc.subject.mesh	Mice
dc.subject.mesh	Gels
dc.subject.mesh	Carum
dc.title	Nigella sativa Oil-loaded Ethanolic Vesicular Gel for Imiquimod-induced Plaque Psoriasis: Physicochemical Characterization, Rheological Studies, and In vivo Efficacy.
dc.type	Journal Article
utslib.citation.volume	22
utslib.location.activity	United Arab Emirates
utslib.for	1115 Pharmacology and Pharmaceutical Sciences
pubs.organisational-group	University of Technology Sydney
pubs.organisational-group	University of Technology Sydney/Faculty of Health
pubs.organisational-group	University of Technology Sydney/UTS Groups
pubs.organisational-group	University of Technology Sydney/UTS Groups/Centre for Inflammation (CFI)
pubs.organisational-group	University of Technology Sydney/UTS Groups/Centre for Inflammation (CFI)/Centre for Inflammation (CFI) Associate Members
pubs.organisational-group	University of Technology Sydney/UTS Groups/Australian Research Consortium in Complementary and Integrative Medicine (ARCCIM)
utslib.copyright.status	in_progress	*
dc.date.updated	2025-01-28T23:28:11Z
pubs.issue	1
pubs.publication-status	Published
pubs.volume	22
utslib.citation.issue	1

Abstract:

BACKGROUND: The therapeutic effect of NS oil in mild to moderate psoriasis is limited owing to low play load of thymoquinone (<15 %w/w), irritation, dripping, low viscosity and thus, less contact time on the lesions. AIMS: This study aimed at developing and characterizing the ethanolic vesicular hydrogel system of Nigella sativa (NS) oil (NS EV hydrogel) for the enhancement of anti-psoriatic activity. OBJECTIVE: The objective of this study was to develop NS EV hydrogel and evaluate its anti-psoriatic activity. METHODS: The identification and quantification of TQ content in different NS seed extracts and marketed oil were measured by an HPTLC method using n-hexane and ethyl acetate as solvent systems. Preparation of ethanolic vesicles (EVs) was performed by solvent injection method, while its antipsoriatic activity was evaluated employing an Imiquad (IMQ)-induced plaque psoriasis animal model. RESULTS: A compact HPTLC band was obtained for TQ at an Rf value of 0.651. The calibration plot was linear in the range of 1-10 μg/spot, and the correlation coefficient of 0.990 was indicative of good linear dependence of peak area on concentration. From the different NS sources, the high TQ content was obtained in the marketed cold press oil, i.e., 1.45±0.08 mg/ml. Out of various NS oilloaded EVs, the F6 formulation revealed the smallest particle size (278.1 nm), with log-normal size distribution (0.459) and adequate entrapment efficiency. A non-uniform shape was observed in the transmission electron microscopy. The viscosity of F6 formulation hydrogel was 32.34 (Pa·s), which exhibited plastic behavior. In vivo, efficacy studies demonstrated decreased inflammation of the epidermis and dermis and a marked decrease in the levels of IL-17 by NS EV hydrogel compared to plain NS oil and standard drugs (Betamethasone and Dr. JRK Psorolin Oil). CONCLUSION: It may be concluded from the findings that NS-loaded EV gel was as good as betamethasone cream but more efficacious than the other treatments.

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