Digital Lasing Biochip for Tumor-Derived Exosome Analysis.

Zhou, T; Fang, G; Wang, Z; Qiao, Z; Nie, N; Fu, B; Tseng, P-H; Sun, X; Chen, Y-C

Digital Lasing Biochip for Tumor-Derived Exosome Analysis.

Zhou, T Fang, G

Wang, Z Qiao, Z Nie, N Fu, B Tseng, P-H Sun, X Chen, Y-C

Permalink

Publisher:: American Chemical Society (ACS)
Publication Type:: Journal Article
Citation:: Anal Chem, 2025, 97, (10), pp. 5605-5611
Issue Date:: 2025-03-18

Open Access

Copyright Clearance Process

Recently Added
In Progress
Open Access

This item is open access.

Adobe PDF

Download Published versionAdobe PDF (4.3 MB)

View on publisher's site

View statistics

Full metadata record

Field	Value	Language
dc.contributor.author	Zhou, T
dc.contributor.author	Fang, G https://orcid.org/0000-0001-5457-7030
dc.contributor.author	Wang, Z
dc.contributor.author	Qiao, Z
dc.contributor.author	Nie, N
dc.contributor.author	Fu, B
dc.contributor.author	Tseng, P-H
dc.contributor.author	Sun, X
dc.contributor.author	Chen, Y-C
dc.date.accessioned	2025-10-02T01:38:33Z
dc.date.available	2025-10-02T01:38:33Z
dc.date.issued	2025-03-18
dc.identifier.citation	Anal Chem, 2025, 97, (10), pp. 5605-5611
dc.identifier.issn	0003-2700
dc.identifier.issn	1520-6882
dc.identifier.uri	http://hdl.handle.net/10453/190285
dc.description.abstract	Digital microfluidics represents an emerging versatile platform that offers numerous advantages in biomolecule detection. However, conventional probes often lack high-intensity and high-sensitivity signals, making it challenging for precise and automatic analysis. Recently, optical microresonators stand as a prominent high-sensitivity detection in the biological field. Here we introduce whispering gallery mode (WGM) microlasers into the microwell array, forming a digital lasing detection system. The lasing signal makes it highly sensitive, which amplifies the subtle changes via the strong interactions of light and matter. The microfluidic droplet technique further allowed microlasers with uniform laser thresholds and high-throughput fabrication. We utilized this tool for the analysis of exosomes derived from tumor spheroids. We believe that this digital optofluidic system could serve as a promising tool in diverse biomolecule assays and various biomedical applications.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	American Chemical Society (ACS)
dc.relation.ispartof	Anal Chem
dc.relation.isbasedon	10.1021/acs.analchem.4c06172
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	0301 Analytical Chemistry, 0399 Other Chemical Sciences
dc.subject.classification	Analytical Chemistry
dc.subject.classification	3205 Medical biochemistry and metabolomics
dc.subject.classification	3401 Analytical chemistry
dc.subject.classification	4004 Chemical engineering
dc.subject.mesh	Exosomes
dc.subject.mesh	Humans
dc.subject.mesh	Microfluidic Analytical Techniques
dc.subject.mesh	Lab-On-A-Chip Devices
dc.subject.mesh	Lasers
dc.subject.mesh	Neoplasms
dc.subject.mesh	Humans
dc.subject.mesh	Neoplasms
dc.subject.mesh	Microfluidic Analytical Techniques
dc.subject.mesh	Lasers
dc.subject.mesh	Exosomes
dc.subject.mesh	Lab-On-A-Chip Devices
dc.subject.mesh	Exosomes
dc.subject.mesh	Humans
dc.subject.mesh	Microfluidic Analytical Techniques
dc.subject.mesh	Lab-On-A-Chip Devices
dc.subject.mesh	Lasers
dc.subject.mesh	Neoplasms
dc.title	Digital Lasing Biochip for Tumor-Derived Exosome Analysis.
dc.type	Journal Article
utslib.citation.volume	97
utslib.location.activity	United States
utslib.for	0301 Analytical Chemistry
utslib.for	0399 Other Chemical Sciences
pubs.organisational-group	University of Technology Sydney
pubs.organisational-group	University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
utslib.copyright.status	open_access	*
dc.rights.license	This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND 4.0). To view a copy of this license, visit https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.date.updated	2025-10-02T01:38:30Z
pubs.issue	10
pubs.publication-status	Published
pubs.volume	97
utslib.citation.issue	10

Abstract:

Digital microfluidics represents an emerging versatile platform that offers numerous advantages in biomolecule detection. However, conventional probes often lack high-intensity and high-sensitivity signals, making it challenging for precise and automatic analysis. Recently, optical microresonators stand as a prominent high-sensitivity detection in the biological field. Here we introduce whispering gallery mode (WGM) microlasers into the microwell array, forming a digital lasing detection system. The lasing signal makes it highly sensitive, which amplifies the subtle changes via the strong interactions of light and matter. The microfluidic droplet technique further allowed microlasers with uniform laser thresholds and high-throughput fabrication. We utilized this tool for the analysis of exosomes derived from tumor spheroids. We believe that this digital optofluidic system could serve as a promising tool in diverse biomolecule assays and various biomedical applications.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/190285