Light field modulation and orthogonal illumination strategies for volumetric imaging

Wu, Sitong

Light field modulation and orthogonal illumination strategies for volumetric imaging

Wu, Sitong

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Publication Type:: Thesis
Issue Date:: 2025

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Field	Value	Language
dc.contributor.author	Wu, Sitong
dc.date.accessioned	2026-02-26T06:33:28Z
dc.date.available	2026-02-26T06:33:28Z
dc.date.issued	2025
dc.identifier.uri	http://hdl.handle.net/10453/193743
dc.description	University of Technology Sydney. Faculty of Science.	en_US.UTF-8
dc.description.abstract	This study explores light field modulation and orthogonal illumination strategies for advanced volumetric imaging. We present three integrated approaches aimed at enhancing resolution, contrast, and imaging depth across biological systems. First, we combine light-sheet fluorescence microscopy with deep learning to achieve high-fidelity 3D vascular imaging in mouse models under low-light conditions, improving signal-to-noise ratio and structural clarity. Second, we develop a rotating oblique illumination scheme based on ROCS (Rotating Oblique-Plane Confocal Scattering) to perform high-speed, label-free 3D scattering tomography of cells and organoids, enabling detailed visualization of subcellular structures. Third, we implement a novel light field modulation technique using multimode fiber to generate a dynamic, scanning light sheet at the distal end, allowing flexible and compact volumetric imaging of cells and organoids in confined or remote environments. Together, these methods demonstrate powerful, scalabl	en_US.UTF-8
dc.format	Thesis (PhD)
dc.language.iso	en	en_US.UTF-8
dc.relation	https://opus.lib.uts.edu.au/bitstream/10453/193743/1/thesis.pdf
dc.rights	info:eu-repo/semantics/openAccess
dc.rights	The author owns the copyright in this thesis including all reproduction and reuse rights for the work. The work may not be altered without the permission of the copyright owner. Attribution is essential when quoting or paraphrasing from this thesis.
dc.rights	©2025 Sitong Wu
dc.rights	au.edu.uts.lib/nph
dc.title	Light field modulation and orthogonal illumination strategies for volumetric imaging	en_US.UTF-8
dc.type	Thesis
utslib.copyright.status	open_access	*

Abstract:

This study explores light field modulation and orthogonal illumination strategies for advanced volumetric imaging. We present three integrated approaches aimed at enhancing resolution, contrast, and imaging depth across biological systems. First, we combine light-sheet fluorescence microscopy with deep learning to achieve high-fidelity 3D vascular imaging in mouse models under low-light conditions, improving signal-to-noise ratio and structural clarity. Second, we develop a rotating oblique illumination scheme based on ROCS (Rotating Oblique-Plane Confocal Scattering) to perform high-speed, label-free 3D scattering tomography of cells and organoids, enabling detailed visualization of subcellular structures. Third, we implement a novel light field modulation technique using multimode fiber to generate a dynamic, scanning light sheet at the distal end, allowing flexible and compact volumetric imaging of cells and organoids in confined or remote environments. Together, these methods demonstrate powerful, scalabl

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http://hdl.handle.net/10453/193743