A portable and versatile rGO-Co3O4-Pt nanocomposite-based electrochemical sensor for ex vivo and in vivo cardiac oxidative stress monitoring.

Singh, S; Melini, S; Raucci, A; Numan, A; Khalid, M; Goh, BH; Meli, R; Pirozzi, C; Cinti, S

A portable and versatile rGO-Co3O4-Pt nanocomposite-based electrochemical sensor for ex vivo and in vivo cardiac oxidative stress monitoring.

Singh, S Melini, S Raucci, A Numan, A Khalid, M Goh, BH Meli, R Pirozzi, C Cinti, S

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Publisher:: Royal Society of Chemistry (RSC)
Publication Type:: Journal Article
Citation:: Nanoscale, 2025, 17, (45), pp. 26417-26428
Issue Date:: 2025-11-20

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Field	Value	Language
dc.contributor.author	Singh, S
dc.contributor.author	Melini, S
dc.contributor.author	Raucci, A
dc.contributor.author	Numan, A
dc.contributor.author	Khalid, M
dc.contributor.author	Goh, BH
dc.contributor.author	Meli, R
dc.contributor.author	Pirozzi, C
dc.contributor.author	Cinti, S
dc.date.accessioned	2026-04-30T00:52:47Z
dc.date.available	2026-04-30T00:52:47Z
dc.date.issued	2025-11-20
dc.identifier.citation	Nanoscale, 2025, 17, (45), pp. 26417-26428
dc.identifier.issn	2040-3364
dc.identifier.issn	2040-3372
dc.identifier.uri	http://hdl.handle.net/10453/194837
dc.description.abstract	The excessive production of reactive oxygen species (ROS) disrupts redox homeostasis, contributing to the development of cardiovascular diseases. Among ROS, hydrogen peroxide (H2O2) serves as a key mediator of oxidative signaling and a critical biomarker of cellular oxidative damage due to its relative stability and signaling relevance. However, current detection strategies for ROS and H2O2 often lack the necessary sensitivity, selectivity, and real-time responsiveness, underscoring the urgent need for advanced sensing platforms to support precision cardiovascular medicine. The research introduces a ROS detection based on reduced graphene oxide-cobalt oxide-platinum (rGO-Co3O4-Pt) nanocomposites for biological sample analysis. The nanocomposite platform enables H2O2 detection down to 160 nM, with linearity up to 2.50 μM. The platform showed acceptable analytical performance in terms of sensitivity, repeatability, and selectivity, which enabled both ex vivo and in vivo H2O2 monitoring. The method was validated in doxorubicin (DOX)-induced cardiotoxicity models using HL-1 cells and C57BL/6J mice, showing strong correlation with MTT-based ROS assays. The rGO-Co3O4-Pt nanocomposite improves portable analytical devices for real-world pharmacological applications. The technology enables real-time therapeutic monitoring while optimizing individualized dosing and expanding its applications to cancer treatment and diabetes management and neurological disorders.
dc.format	Electronic
dc.language	eng
dc.publisher	Royal Society of Chemistry (RSC)
dc.relation.ispartof	Nanoscale
dc.relation.isbasedon	10.1039/d5nr03297k
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	02 Physical Sciences, 03 Chemical Sciences, 10 Technology
dc.subject.classification	Nanoscience & Nanotechnology
dc.subject.classification	34 Chemical sciences
dc.subject.classification	40 Engineering
dc.subject.classification	51 Physical sciences
dc.subject.mesh	Animals
dc.subject.mesh	Nanocomposites
dc.subject.mesh	Graphite
dc.subject.mesh	Platinum
dc.subject.mesh	Cobalt
dc.subject.mesh	Oxidative Stress
dc.subject.mesh	Mice
dc.subject.mesh	Oxides
dc.subject.mesh	Hydrogen Peroxide
dc.subject.mesh	Doxorubicin
dc.subject.mesh	Mice, Inbred C57BL
dc.subject.mesh	Electrochemical Techniques
dc.subject.mesh	Cell Line
dc.subject.mesh	Reactive Oxygen Species
dc.subject.mesh	Cell Line
dc.subject.mesh	Animals
dc.subject.mesh	Mice, Inbred C57BL
dc.subject.mesh	Mice
dc.subject.mesh	Oxides
dc.subject.mesh	Hydrogen Peroxide
dc.subject.mesh	Graphite
dc.subject.mesh	Cobalt
dc.subject.mesh	Platinum
dc.subject.mesh	Reactive Oxygen Species
dc.subject.mesh	Doxorubicin
dc.subject.mesh	Oxidative Stress
dc.subject.mesh	Nanocomposites
dc.subject.mesh	Electrochemical Techniques
dc.subject.mesh	Animals
dc.subject.mesh	Nanocomposites
dc.subject.mesh	Graphite
dc.subject.mesh	Platinum
dc.subject.mesh	Cobalt
dc.subject.mesh	Oxidative Stress
dc.subject.mesh	Mice
dc.subject.mesh	Oxides
dc.subject.mesh	Hydrogen Peroxide
dc.subject.mesh	Doxorubicin
dc.subject.mesh	Mice, Inbred C57BL
dc.subject.mesh	Electrochemical Techniques
dc.subject.mesh	Cell Line
dc.subject.mesh	Reactive Oxygen Species
dc.title	A portable and versatile rGO-Co3O4-Pt nanocomposite-based electrochemical sensor for ex vivo and in vivo cardiac oxidative stress monitoring.
dc.type	Journal Article
utslib.citation.volume	17
utslib.location.activity	England
utslib.for	02 Physical Sciences
utslib.for	03 Chemical Sciences
utslib.for	10 Technology
pubs.organisational-group	University of Technology Sydney
pubs.organisational-group	University of Technology Sydney/Faculty of Health
utslib.copyright.status	open_access	*
dc.rights.license	This work is licensed under a Creative Commons Attribution 3.0 Unported License (CC BY 3.0). To view a copy of this license, visit https://creativecommons.org/licenses/by/3.0/.
dc.date.updated	2026-04-30T00:52:45Z
pubs.issue	45
pubs.publication-status	Published online
pubs.volume	17
utslib.citation.issue	45

Abstract:

The excessive production of reactive oxygen species (ROS) disrupts redox homeostasis, contributing to the development of cardiovascular diseases. Among ROS, hydrogen peroxide (H2O2) serves as a key mediator of oxidative signaling and a critical biomarker of cellular oxidative damage due to its relative stability and signaling relevance. However, current detection strategies for ROS and H2O2 often lack the necessary sensitivity, selectivity, and real-time responsiveness, underscoring the urgent need for advanced sensing platforms to support precision cardiovascular medicine. The research introduces a ROS detection based on reduced graphene oxide-cobalt oxide-platinum (rGO-Co3O4-Pt) nanocomposites for biological sample analysis. The nanocomposite platform enables H2O2 detection down to 160 nM, with linearity up to 2.50 μM. The platform showed acceptable analytical performance in terms of sensitivity, repeatability, and selectivity, which enabled both ex vivo and in vivo H2O2 monitoring. The method was validated in doxorubicin (DOX)-induced cardiotoxicity models using HL-1 cells and C57BL/6J mice, showing strong correlation with MTT-based ROS assays. The rGO-Co3O4-Pt nanocomposite improves portable analytical devices for real-world pharmacological applications. The technology enables real-time therapeutic monitoring while optimizing individualized dosing and expanding its applications to cancer treatment and diabetes management and neurological disorders.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/194837