Advanced 3d Bioprinted Conductive Tissue for Cardiac Regeneration

Al Shamery, Wafa

Advanced 3d Bioprinted Conductive Tissue for Cardiac Regeneration

Al Shamery, Wafa

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Publication Type:: Thesis
Issue Date:: 2025

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Field	Value	Language
dc.contributor.author	Al Shamery, Wafa
dc.date.accessioned	2026-07-21T01:09:58Z
dc.date.available	2026-07-21T01:09:58Z
dc.date.issued	2025
dc.identifier.uri	http://hdl.handle.net/10453/195726
dc.description	University of Technology Sydney. Faculty of Engineering and Information Technology.	en_US.UTF-8
dc.description.abstract	This thesis explores the use of conductive polymers (CPs) and hydrogels in cardiac tissue engineering, focusing on their potential to repair and regenerate myocardial tissue. The role of CPs in enhancing the electrical conductivity and mechanical properties of engineered cardiac tissues is central to this research. Previous studies in our team developed alginate-gelatin (Alg-Gel) hydrogels for cardiac tissue engineering applications. In this study, I aimed to improve the conductivity of Alg-Gel hydrogels by incorporating and comparing conductive materials, such as graphene, graphene oxide, gold nanoparticles, polypyrrole, and polyaniline. First, these hydrogels were evaluated for their effects on conductivity, pore size, printability and durability of Alg-Gel hydrogels. Then, they were tested with freely dispersed cardiac cells, as well as in the presence of cardiac spheroids, a three-dimensional in vitro culture model of the heart, for their effects on cell viability and toxicity. The conductive hydrogels demonstrated good biocompatibility, supporting high cell viability and promoting the formation of functional cardiac tissue. In vivo results demonstrated that conductive hydrogel-based patches containing G and GO improved myocardial function, as evidenced by increased ejection fraction and reduced fibrosis by day 28 post-implantation. These findings suggest that conductive hydrogels offer promise for cardiac tissue engineering.	en_US.UTF-8
dc.format	Thesis (PhD)
dc.language.iso	en_US	en_US.UTF-8
dc.relation	https://opus.lib.uts.edu.au/bitstream/10453/195726/3/thesis.pdf
dc.rights	info:eu-repo/semantics/openAccess
dc.rights	The author owns the copyright in this thesis including all reproduction and reuse rights for the work. The work may not be altered without the permission of the copyright owner. Attribution is essential when quoting or paraphrasing from this thesis.
dc.rights	© 2025 Wafa Al Shamery
dc.rights	au.edu.uts.lib/cph
dc.title	Advanced 3d Bioprinted Conductive Tissue for Cardiac Regeneration	en_US.UTF-8
dc.type	Thesis
utslib.copyright.status	open_access	*

Abstract:

This thesis explores the use of conductive polymers (CPs) and hydrogels in cardiac tissue engineering, focusing on their potential to repair and regenerate myocardial tissue. The role of CPs in enhancing the electrical conductivity and mechanical properties of engineered cardiac tissues is central to this research. Previous studies in our team developed alginate-gelatin (Alg-Gel) hydrogels for cardiac tissue engineering applications. In this study, I aimed to improve the conductivity of Alg-Gel hydrogels by incorporating and comparing conductive materials, such as graphene, graphene oxide, gold nanoparticles, polypyrrole, and polyaniline. First, these hydrogels were evaluated for their effects on conductivity, pore size, printability and durability of Alg-Gel hydrogels. Then, they were tested with freely dispersed cardiac cells, as well as in the presence of cardiac spheroids, a three-dimensional in vitro culture model of the heart, for their effects on cell viability and toxicity. The conductive hydrogels demonstrated good biocompatibility, supporting high cell viability and promoting the formation of functional cardiac tissue. In vivo results demonstrated that conductive hydrogel-based patches containing G and GO improved myocardial function, as evidenced by increased ejection fraction and reduced fibrosis by day 28 post-implantation. These findings suggest that conductive hydrogels offer promise for cardiac tissue engineering.

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http://hdl.handle.net/10453/195726