Label-free impedimetric immunosensor for sensitive detection of 2,4-dichlorophenoxybutyric acid (2,4-DB) in soybean

Publisher:
Elsevier Inc
Publication Type:
Journal Article
Citation:
Talanta, 2012, 101 pp. 226 - 232
Issue Date:
2012-01
Full metadata record
Files in This Item:
Filename Description Size
Thumbnail2012000480OK.pdf751.83 kB
Adobe PDF
Electrochemical impedance immunosensor, with its high sensitivity from electrochemical impedance analysis and ideal specificity from the immunoassay, is increasingly used in the detection of a kind of phenoxy acid herbicides which is 2,4-Dichlorophenoxybutyric acid (2,4-DB). In this experiment, synthetic 2,4-DB antibodies were immobilized on the electrode by the crosslinking of l-Cysteine/glutaraldehyde, and 2,4-DB were measured by the increase of electron-transfer resistance when the immune reaction occurred, with Fe(CN)63â/Fe(CN)64â as the probe. Under optimal conditions, the change of resistance is in a linear relationship with the logarithm of the concentration in the range of 1.0Ã10â7â1.0Ã10â3 g/L (R=0.994) with the detection limit of 1.0Ã10â7 g/L (0.1 ppb). This method bears such merits as simplicity in operation, high sensitivity, wide linear range, specificity, reproducibility and good stability. The actual soybean samples were analyzed with the recovery of 82.8%â102.3%.
Please use this identifier to cite or link to this item: