A fast CE method for the achiral separation of methadone and its major metabolites, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine and 2-ethyl-5-methyl-3,3-diphenyl-1-pyrroline

Kelly, T; Doble, P; Dawson, M

A fast CE method for the achiral separation of methadone and its major metabolites, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine and 2-ethyl-5-methyl-3,3-diphenyl-1-pyrroline

Kelly, T

Doble, P

Dawson, M

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Publication Type:: Journal Article
Citation:: Electrophoresis, 2007, 28 (19), pp. 3566 - 3569
Issue Date:: 2007-10-01

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Field	Value	Language
dc.contributor.author	Kelly, T https://orcid.org/0000-0002-8946-9686	en_US
dc.contributor.author	Doble, P https://orcid.org/0000-0002-8472-1301	en_US
dc.contributor.author	Dawson, M	en_US
dc.date.issued	2007-10-01	en_US
dc.identifier.citation	Electrophoresis, 2007, 28 (19), pp. 3566 - 3569	en_US
dc.identifier.issn	0173-0835	en_US
dc.identifier.uri	http://hdl.handle.net/10453/3213
dc.description.abstract	The utilization of dynamic doubly coated capillaries for a fast separation of methadone and its two major metabolites, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) and 2-ethyl-5-methyl-3,3-diphenyl-1-pyrroline (EMDP) was investigated. The coated capillaries were prepared using a polycation of poly(diallyldimethylammonium chloride) and a polyanion of dextran sulfate. A fast achiral separation was developed using the coated capillaries with a BGE of 100 mM phosphate buffer at pH 2.6. Complete achiral separation of methadone, EDDP and EMDP was achieved, with migration times of approximately 4 min. The method offers considerable advantages with respect to BGE simplicity and analysis time compared to previously published CE methods for methadone and its related analytes. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.	en_US
dc.relation.ispartof	Electrophoresis	en_US
dc.relation.isbasedon	10.1002/elps.200700090	en_US
dc.subject.classification	Analytical Chemistry	en_US
dc.subject.mesh	Phosphates	en_US
dc.subject.mesh	Polyethylenes	en_US
dc.subject.mesh	Methadone	en_US
dc.subject.mesh	Pyrrolidines	en_US
dc.subject.mesh	Dextran Sulfate	en_US
dc.subject.mesh	Coated Materials, Biocompatible	en_US
dc.subject.mesh	Calibration	en_US
dc.subject.mesh	Electrophoresis, Capillary	en_US
dc.subject.mesh	Spectrophotometry, Ultraviolet	en_US
dc.subject.mesh	Sensitivity and Specificity	en_US
dc.subject.mesh	Reproducibility of Results	en_US
dc.subject.mesh	Hydrogen-Ion Concentration	en_US
dc.subject.mesh	Quaternary Ammonium Compounds	en_US
dc.subject.mesh	Forensic Toxicology	en_US
dc.title	A fast CE method for the achiral separation of methadone and its major metabolites, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine and 2-ethyl-5-methyl-3,3-diphenyl-1-pyrroline	en_US
dc.type	Journal Article
utslib.citation.volume	19	en_US
utslib.citation.volume	28	en_US
utslib.for	0301 Analytical Chemistry	en_US
utslib.for	0601 Biochemistry and Cell Biology	en_US
utslib.for	0904 Chemical Engineering	en_US
dc.location.activity	ISI:000250294200023	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Chemistry and Forensic Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CFS - Centre for Forensic Science
utslib.copyright.status	closed_access
pubs.issue	19	en_US
pubs.publication-status	Published	en_US
pubs.volume	28	en_US

Abstract:

The utilization of dynamic doubly coated capillaries for a fast separation of methadone and its two major metabolites, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) and 2-ethyl-5-methyl-3,3-diphenyl-1-pyrroline (EMDP) was investigated. The coated capillaries were prepared using a polycation of poly(diallyldimethylammonium chloride) and a polyanion of dextran sulfate. A fast achiral separation was developed using the coated capillaries with a BGE of 100 mM phosphate buffer at pH 2.6. Complete achiral separation of methadone, EDDP and EMDP was achieved, with migration times of approximately 4 min. The method offers considerable advantages with respect to BGE simplicity and analysis time compared to previously published CE methods for methadone and its related analytes. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

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http://hdl.handle.net/10453/3213