A rapid CZE method for the analysis of benzodiazepines in spiked beverages

Webb, R; Doble, P; Dawson, M

A rapid CZE method for the analysis of benzodiazepines in spiked beverages

Webb, R Doble, P

Dawson, M

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Publication Type:: Journal Article
Citation:: Electrophoresis, 2007, 28 (19), pp. 3553 - 3565
Issue Date:: 2007-10-01

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Field	Value	Language
dc.contributor.author	Webb, R	en_US
dc.contributor.author	Doble, P https://orcid.org/0000-0002-8472-1301	en_US
dc.contributor.author	Dawson, M	en_US
dc.date.issued	2007-10-01	en_US
dc.identifier.citation	Electrophoresis, 2007, 28 (19), pp. 3553 - 3565	en_US
dc.identifier.issn	0173-0835	en_US
dc.identifier.uri	http://hdl.handle.net/10453/3217
dc.description.abstract	A rapid CZE method was developed for the simultaneous determination of nine benzodiazepines in spiked beverages (nitrazepam oxazepam, alprazolam, flunitrazepam, temazepam, diazepam, 7-aminoflunitrazepam, 7-aminonitrazepam and 7-aminoclonazepam). The method employed a double-coated capillary coated with poly(diallyldimethylammonium chloride) and then dextran sulphate. The BGE conditions were 100 mM ammonium phosphate buffer, pH 2.5, which gave baseline resolution between consecutive peaks and a run time of less than 6.5 min. This method offers improvements in both resolution and run time, compared to those attained under analogous conditions with an uncoated capillary. The validated method was successfully applied to beverages that had been spiked with benzodiazepines at concentrations simulating prescription tablets. No sample pretreatment was required to quantify five benzodiazepines in Coca-Cola, orange juice, beer, bourbon and Bacardi. The exception was white wine, where the complex sample matrix did not enable the accurate quantification of nitrazepam. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.	en_US
dc.relation.ispartof	Electrophoresis	en_US
dc.relation.isbasedon	10.1002/elps.200700110	en_US
dc.subject.classification	Analytical Chemistry	en_US
dc.subject.mesh	Phosphates	en_US
dc.subject.mesh	Polyethylenes	en_US
dc.subject.mesh	Benzodiazepines	en_US
dc.subject.mesh	Dextran Sulfate	en_US
dc.subject.mesh	Anti-Anxiety Agents	en_US
dc.subject.mesh	Coated Materials, Biocompatible	en_US
dc.subject.mesh	Calibration	en_US
dc.subject.mesh	Electrophoresis, Capillary	en_US
dc.subject.mesh	Spectrophotometry, Ultraviolet	en_US
dc.subject.mesh	Sensitivity and Specificity	en_US
dc.subject.mesh	Reproducibility of Results	en_US
dc.subject.mesh	Hydrogen-Ion Concentration	en_US
dc.subject.mesh	Beverages	en_US
dc.subject.mesh	Alcoholic Beverages	en_US
dc.subject.mesh	Quaternary Ammonium Compounds	en_US
dc.subject.mesh	Forensic Toxicology	en_US
dc.title	A rapid CZE method for the analysis of benzodiazepines in spiked beverages	en_US
dc.type	Journal Article
utslib.citation.volume	19	en_US
utslib.citation.volume	28	en_US
utslib.for	0399 Other Chemical Sciences	en_US
utslib.for	0301 Analytical Chemistry	en_US
utslib.for	0601 Biochemistry and Cell Biology	en_US
utslib.for	0904 Chemical Engineering	en_US
dc.location.activity	ISI:000250294200022	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Chemistry and Forensic Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CFS - Centre for Forensic Science
utslib.copyright.status	closed_access
pubs.issue	19	en_US
pubs.publication-status	Published	en_US
pubs.volume	28	en_US

Abstract:

A rapid CZE method was developed for the simultaneous determination of nine benzodiazepines in spiked beverages (nitrazepam oxazepam, alprazolam, flunitrazepam, temazepam, diazepam, 7-aminoflunitrazepam, 7-aminonitrazepam and 7-aminoclonazepam). The method employed a double-coated capillary coated with poly(diallyldimethylammonium chloride) and then dextran sulphate. The BGE conditions were 100 mM ammonium phosphate buffer, pH 2.5, which gave baseline resolution between consecutive peaks and a run time of less than 6.5 min. This method offers improvements in both resolution and run time, compared to those attained under analogous conditions with an uncoated capillary. The validated method was successfully applied to beverages that had been spiked with benzodiazepines at concentrations simulating prescription tablets. No sample pretreatment was required to quantify five benzodiazepines in Coca-Cola, orange juice, beer, bourbon and Bacardi. The exception was white wine, where the complex sample matrix did not enable the accurate quantification of nitrazepam. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

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http://hdl.handle.net/10453/3217