Reductive transformation of temazepam and lorazepam during b-glucuronidase treatment of urine specimens

Publisher:
GI Printing & Graphics
Publication Type:
Conference Proceeding
Citation:
TIAFT Bulletin, 2011, 41 pp. 53 - 55
Issue Date:
2011-01
Full metadata record
Files in This Item:
Filename Description Size
Thumbnail2011000907NPR.pdf Published version961 kB
Adobe PDF
B-Glucuronidase is on enzyme often employed to deconjugate B-glucuronides during urinary drug testing for benzodiozepines. It is commonly accepted that use of B-glucuronidase is a preferred method of hydrolysis over acid-catalyzed hydrolysis, which is known to induce benzodiozepine degradation and transformation. Very recently, we have reported that B-glucuronidase catalyzed hydrolysis is also a source of artefact production. We found that either oxazepam glucuronide present in patient urine or free oxazepam added in blank urine could be reduced to nordiozepom (desmethyldiozepom) during incubation with commercial B-glucuronidase enzymes. Formation of nordiozepom artefact was positively correlated with incubation temperature, incubation time, oxazepam concentration and enzyme concentration.
Please use this identifier to cite or link to this item: