Reductive transformation of temazepam and lorazepam during b-glucuronidase treatment of urine specimens

Fu, S; Molnar, A; Bowron, P; Wang, H; Dawson, M; Lewis, JH

Reductive transformation of temazepam and lorazepam during b-glucuronidase treatment of urine specimens

Fu, S

Molnar, A Bowron, P Wang, H Dawson, M Lewis, JH

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Publisher:: GI Printing & Graphics
Publication Type:: Conference Proceeding
Citation:: TIAFT Bulletin, 2011, 41 pp. 53 - 55
Issue Date:: 2011-01

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Field	Value	Language
dc.contributor.author	Fu, S https://orcid.org/0000-0002-6238-3612	en_US
dc.contributor.author	Molnar, A	en_US
dc.contributor.author	Bowron, P	en_US
dc.contributor.author	Wang, H	en_US
dc.contributor.author	Dawson, M	en_US
dc.contributor.author	Lewis, JH	en_US
dc.contributor.editor	Gerostamoulos, D	en_US
dc.contributor.editor	Beyer, J	en_US
dc.date	2010-08-29	en_US
dc.date.issued	2011-01	en_US
dc.identifier.citation	TIAFT Bulletin, 2011, 41 pp. 53 - 55	en_US
dc.identifier.uri	http://hdl.handle.net/10453/33309
dc.description.abstract	B-Glucuronidase is on enzyme often employed to deconjugate B-glucuronides during urinary drug testing for benzodiozepines. It is commonly accepted that use of B-glucuronidase is a preferred method of hydrolysis over acid-catalyzed hydrolysis, which is known to induce benzodiozepine degradation and transformation. Very recently, we have reported that B-glucuronidase catalyzed hydrolysis is also a source of artefact production. We found that either oxazepam glucuronide present in patient urine or free oxazepam added in blank urine could be reduced to nordiozepom (desmethyldiozepom) during incubation with commercial B-glucuronidase enzymes. Formation of nordiozepom artefact was positively correlated with incubation temperature, incubation time, oxazepam concentration and enzyme concentration.	en_US
dc.publisher	GI Printing & Graphics	en_US
dc.relation.ispartof	TIAFT Bulletin	en_US
dc.relation.ispartof	Annual Meeting of the International-Association-of-Forensic-Toxicologists (TIAFT)	en_US
dc.title	Reductive transformation of temazepam and lorazepam during b-glucuronidase treatment of urine specimens	en_US
dc.type	Conference Proceeding
utslib.citation.volume	41	en_US
utslib.location	Australia	en_US
utslib.location.activity	Germany	en_US
utslib.for	110107 Metabolic Medicine	en_US
dc.location.activity	Germany
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Chemistry and Forensic Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CCET - Centre for Clean Energy Technology
pubs.organisational-group	/University of Technology Sydney/Strength - CFS - Centre for Forensic Science
utslib.copyright.status	closed_access
pubs.consider-herdc	false	en_US
pubs.finish-date	2010-09-02	en_US
pubs.place-of-publication	Australia	en_US
pubs.publication-status	Published	en_US
pubs.start-date	2010-08-29	en_US
pubs.volume	41	en_US

Abstract:

B-Glucuronidase is on enzyme often employed to deconjugate B-glucuronides during urinary drug testing for benzodiozepines. It is commonly accepted that use of B-glucuronidase is a preferred method of hydrolysis over acid-catalyzed hydrolysis, which is known to induce benzodiozepine degradation and transformation. Very recently, we have reported that B-glucuronidase catalyzed hydrolysis is also a source of artefact production. We found that either oxazepam glucuronide present in patient urine or free oxazepam added in blank urine could be reduced to nordiozepom (desmethyldiozepom) during incubation with commercial B-glucuronidase enzymes. Formation of nordiozepom artefact was positively correlated with incubation temperature, incubation time, oxazepam concentration and enzyme concentration.

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http://hdl.handle.net/10453/33309