Reductive transformation of temazepam and lorazepam during b-glucuronidase treatment of urine specimens

GI Printing & Graphics
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Conference Proceeding
TIAFT Bulletin, 2011, 41 pp. 53 - 55
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B-Glucuronidase is on enzyme often employed to deconjugate B-glucuronides during urinary drug testing for benzodiozepines. It is commonly accepted that use of B-glucuronidase is a preferred method of hydrolysis over acid-catalyzed hydrolysis, which is known to induce benzodiozepine degradation and transformation. Very recently, we have reported that B-glucuronidase catalyzed hydrolysis is also a source of artefact production. We found that either oxazepam glucuronide present in patient urine or free oxazepam added in blank urine could be reduced to nordiozepom (desmethyldiozepom) during incubation with commercial B-glucuronidase enzymes. Formation of nordiozepom artefact was positively correlated with incubation temperature, incubation time, oxazepam concentration and enzyme concentration.
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