Optimisation for the separation of the oligosaccharide, sodium Pentosan Polysulfate by reverse polarity capillary zone electrophoresis using a central composite design

Prochazka, S; Mulholland, M; Lloyd-Jones, A

Optimisation for the separation of the oligosaccharide, sodium Pentosan Polysulfate by reverse polarity capillary zone electrophoresis using a central composite design

Prochazka, S Mulholland, M Lloyd-Jones, A

Permalink

Publication Type:: Journal Article
Citation:: Journal of Pharmaceutical and Biomedical Analysis, 2003, 31 (1), pp. 133 - 141
Issue Date:: 2003-02-05

Closed Access

	Filename	Description	Size
	2003000445.pdf		716.73 kB	Adobe PDF	View/Open

Copyright Clearance Process

Recently Added
In Progress
Closed Access

This item is closed access and not available.

Full metadata record

Field	Value	Language
dc.contributor.author	Prochazka, S	en_US
dc.contributor.author	Mulholland, M	en_US
dc.contributor.author	Lloyd-Jones, A	en_US
dc.date.issued	2003-02-05	en_US
dc.identifier.citation	Journal of Pharmaceutical and Biomedical Analysis, 2003, 31 (1), pp. 133 - 141	en_US
dc.identifier.issn	0731-7085	en_US
dc.identifier.uri	http://hdl.handle.net/10453/3573
dc.description.abstract	The separation by reverse polarity capillary zone electrophoresis of the therapeutically developed sodium salt of Pentosan Polysulfate was optimised through the analysis of response surface methodologies, modeled using a central composite design. The optimisation investigated injection pressure, injection time and voltage and the effect of the conditions on retention times, peak areas, separation efficiency and the method sensitivity. The overall goal was to develop the most sensitive results with no decrease in separation efficiency. The following results were obtained: (1) retention times generally decreased as injection pressure, injection time and voltage increased, injection time having the least effect; (2) as expected peak areas increased as injection pressure and injection time increased but decreased as voltage increased; (3) separation efficiencies generally increased as injection pressure and injection time decreased, with voltage having almost no effect. For the optimum condition, the sample was introduced at the inlet vial at the cathode hydrodynamically, at optimal setting of 44 s at 35 mbar. The optimal voltage was -20 kV. In comparison with other methods, the optimum showed increased sensitivity, resolution and separation efficiency. Repeatability studies were performed on the optimum parameter conditions. Relative standard deviation values obtained were between 0.9 and 5.4%. © 2002 Elsevier Science B.V. All rights reserved.	en_US
dc.relation.ispartof	Journal of Pharmaceutical and Biomedical Analysis	en_US
dc.relation.isbasedon	10.1016/S0731-7085(02)00569-1	en_US
dc.subject.classification	Analytical Chemistry	en_US
dc.subject.mesh	Electrolytes	en_US
dc.subject.mesh	Pentosan Sulfuric Polyester	en_US
dc.subject.mesh	Indicators and Reagents	en_US
dc.subject.mesh	Chromatography, High Pressure Liquid	en_US
dc.subject.mesh	Electrophoresis, Capillary	en_US
dc.subject.mesh	Spectrophotometry, Ultraviolet	en_US
dc.subject.mesh	Reproducibility of Results	en_US
dc.subject.mesh	Carbohydrate Sequence	en_US
dc.subject.mesh	Molecular Sequence Data	en_US
dc.title	Optimisation for the separation of the oligosaccharide, sodium Pentosan Polysulfate by reverse polarity capillary zone electrophoresis using a central composite design	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.citation.volume	31	en_US
utslib.for	0301 Analytical Chemistry	en_US
utslib.for	1115 Pharmacology and Pharmaceutical Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Chemistry and Forensic Science
utslib.copyright.status	closed_access
pubs.issue	1	en_US
pubs.publication-status	Published	en_US
pubs.volume	31	en_US

Abstract:

The separation by reverse polarity capillary zone electrophoresis of the therapeutically developed sodium salt of Pentosan Polysulfate was optimised through the analysis of response surface methodologies, modeled using a central composite design. The optimisation investigated injection pressure, injection time and voltage and the effect of the conditions on retention times, peak areas, separation efficiency and the method sensitivity. The overall goal was to develop the most sensitive results with no decrease in separation efficiency. The following results were obtained: (1) retention times generally decreased as injection pressure, injection time and voltage increased, injection time having the least effect; (2) as expected peak areas increased as injection pressure and injection time increased but decreased as voltage increased; (3) separation efficiencies generally increased as injection pressure and injection time decreased, with voltage having almost no effect. For the optimum condition, the sample was introduced at the inlet vial at the cathode hydrodynamically, at optimal setting of 44 s at 35 mbar. The optimal voltage was -20 kV. In comparison with other methods, the optimum showed increased sensitivity, resolution and separation efficiency. Repeatability studies were performed on the optimum parameter conditions. Relative standard deviation values obtained were between 0.9 and 5.4%. © 2002 Elsevier Science B.V. All rights reserved.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/3573