Isolation of small noncoding RNAS from human serum

Publication Type:
Journal Article
Journal of Visualized Experiments, 2014, (88)
Issue Date:
Full metadata record
This protocol describes a method for extracting small RNAs from human serum. We have used this method to isolate microRNAs from cancer serum for use in DNA arrays and also singleplex quantitative PCR. The protocol utilizes phenol and guanidinium thiocyanate reagents with modifications to yield high quality RNA.
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