Isolation of small noncoding RNAS from human serum

Khoury, S; Ajuyah, P; Tran, N

Isolation of small noncoding RNAS from human serum

Khoury, S

Ajuyah, P Tran, N

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Publication Type:: Journal Article
Citation:: Journal of Visualized Experiments, 2014, (88)
Issue Date:: 2014-06-19

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Field	Value	Language
dc.contributor.author	Khoury, S https://orcid.org/0000-0001-5631-7478	en_US
dc.contributor.author	Ajuyah, P	en_US
dc.contributor.author	Tran, N https://orcid.org/0000-0001-7747-2530	en_US
dc.date.available	2020-05-25T19:11:47Z
dc.date.issued	2014-06-19	en_US
dc.identifier.citation	Journal of Visualized Experiments, 2014, (88)	en_US
dc.identifier.issn	1940-087X	en_US
dc.identifier.uri	http://hdl.handle.net/10453/36268
dc.description.abstract	This protocol describes a method for extracting small RNAs from human serum. We have used this method to isolate microRNAs from cancer serum for use in DNA arrays and also singleplex quantitative PCR. The protocol utilizes phenol and guanidinium thiocyanate reagents with modifications to yield high quality RNA.	en_US
dc.relation.ispartof	Journal of Visualized Experiments	en_US
dc.relation.isbasedon	10.3791/51443	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	RNA, Untranslated	en_US
dc.subject.mesh	Blood Chemical Analysis	en_US
dc.title	Isolation of small noncoding RNAS from human serum	en_US
dc.type	Journal Article
utslib.citation.volume	88	en_US
utslib.for	0699 Other Biological Sciences	en_US
utslib.for	0601 Biochemistry and Cell Biology	en_US
utslib.for	1701 Psychology	en_US
utslib.for	1702 Cognitive Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
utslib.copyright.status	open_access
pubs.issue	88	en_US
pubs.publication-status	Published	en_US

Abstract:

This protocol describes a method for extracting small RNAs from human serum. We have used this method to isolate microRNAs from cancer serum for use in DNA arrays and also singleplex quantitative PCR. The protocol utilizes phenol and guanidinium thiocyanate reagents with modifications to yield high quality RNA.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/36268