Temporal dynamics and subpopulation analysis of Theileria orientalis genotypes in cattle

Jenkins, C; Micallef, M; Alex, SM; Collins, D; Djordjevic, SP; Bogema, DR

Temporal dynamics and subpopulation analysis of Theileria orientalis genotypes in cattle

Jenkins, C

Micallef, M Alex, SM Collins, D Djordjevic, SP

Bogema, DR

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Publication Type:: Journal Article
Citation:: Infection, Genetics and Evolution, 2015, 32 pp. 199 - 207
Issue Date:: 2015-06-01

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Field	Value	Language
dc.contributor.author	Jenkins, C https://orcid.org/0000-0002-5966-072X	en_US
dc.contributor.author	Micallef, M	en_US
dc.contributor.author	Alex, SM	en_US
dc.contributor.author	Collins, D	en_US
dc.contributor.author	Djordjevic, SP https://orcid.org/0000-0001-9301-5372	en_US
dc.contributor.author	Bogema, DR https://orcid.org/0000-0001-7013-7326	en_US
dc.date.available	2015-03-12	en_US
dc.date.issued	2015-06-01	en_US
dc.identifier.citation	Infection, Genetics and Evolution, 2015, 32 pp. 199 - 207	en_US
dc.identifier.issn	1567-1348	en_US
dc.identifier.uri	http://hdl.handle.net/10453/36818
dc.identifier.uri	http://hdl.handle.net/10453/36819
dc.identifier.uri	http://hdl.handle.net/10453/36817
dc.description.abstract	© 2015 Elsevier B.V. In Australia, outbreaks of clinical theileriosis caused by Theileria orientalis have been largely associated with the Ikeda genotype which can occur as a sole infection, or more commonly, as a mixture of genotypes. The most prevalent genotype, Chitose, frequently co-occurs with type Ikeda, however the role of this genotype in clinical disease has not been clearly established. Furthermore, the dynamics of individual genotypes in field infection of cattle have not been examined. In this study we developed quantitative PCR (qPCR) and genotyping methods to examine the role of the Chitose genotype in clinical disease and to investigate the temporal dynamics of T. orientalis Ikeda, Chitose and Buffeli genotypes in naïve animals introduced to a T. orientalis-endemic area. Analysis of the major piroplasm surface protein (MPSP) genes of Chitose isolates revealed the presence of two distinct phylogenetic clusters, Chitose A and Chitose B. A genotyping assay aimed at determining Chitose A/B allele frequency revealed that the Chitose A phylogenetic cluster is strongly associated with clinical disease but nearly always co-occurs with the Ikeda genotype. qPCR revealed that the Chitose genotype (particularly Chitose A), undergoes temporal switching in conjunction with the Ikeda genotype and contributes substantially to the overall parasite burden. The benign Buffeli genotype can also undergo temporal switching but levels of this genotype appear to remain low relative to the Ikeda and Chitose types. Interplay between vector and host immunological factors is presumed to be critical to the population dynamics observed in this study. Genotypic switching likely contributes to the persistence of T. orientalis in the host.	en_US
dc.relation.ispartof	Infection, Genetics and Evolution	en_US
dc.relation.isbasedon	10.1016/j.meegid.2015.03.017	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Cattle	en_US
dc.subject.mesh	Theileria	en_US
dc.subject.mesh	Theileriasis	en_US
dc.subject.mesh	Cattle Diseases	en_US
dc.subject.mesh	DNA, Protozoan	en_US
dc.subject.mesh	Polymerase Chain Reaction	en_US
dc.subject.mesh	Sequence Analysis, DNA	en_US
dc.subject.mesh	Disease Outbreaks	en_US
dc.subject.mesh	Phylogeny	en_US
dc.subject.mesh	Genotype	en_US
dc.subject.mesh	Australia	en_US
dc.subject.mesh	Phylogeography	en_US
dc.subject.mesh	Genotyping Techniques	en_US
dc.title	Temporal dynamics and subpopulation analysis of Theileria orientalis genotypes in cattle	en_US
dc.type	Journal Article
utslib.citation.volume	32	en_US
utslib.for	0604 Genetics	en_US
utslib.for	0707 Veterinary Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Medical and Molecular Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	open_access
pubs.publication-status	Published	en_US
pubs.volume	32	en_US

Abstract:

© 2015 Elsevier B.V. In Australia, outbreaks of clinical theileriosis caused by Theileria orientalis have been largely associated with the Ikeda genotype which can occur as a sole infection, or more commonly, as a mixture of genotypes. The most prevalent genotype, Chitose, frequently co-occurs with type Ikeda, however the role of this genotype in clinical disease has not been clearly established. Furthermore, the dynamics of individual genotypes in field infection of cattle have not been examined. In this study we developed quantitative PCR (qPCR) and genotyping methods to examine the role of the Chitose genotype in clinical disease and to investigate the temporal dynamics of T. orientalis Ikeda, Chitose and Buffeli genotypes in naïve animals introduced to a T. orientalis-endemic area. Analysis of the major piroplasm surface protein (MPSP) genes of Chitose isolates revealed the presence of two distinct phylogenetic clusters, Chitose A and Chitose B. A genotyping assay aimed at determining Chitose A/B allele frequency revealed that the Chitose A phylogenetic cluster is strongly associated with clinical disease but nearly always co-occurs with the Ikeda genotype. qPCR revealed that the Chitose genotype (particularly Chitose A), undergoes temporal switching in conjunction with the Ikeda genotype and contributes substantially to the overall parasite burden. The benign Buffeli genotype can also undergo temporal switching but levels of this genotype appear to remain low relative to the Ikeda and Chitose types. Interplay between vector and host immunological factors is presumed to be critical to the population dynamics observed in this study. Genotypic switching likely contributes to the persistence of T. orientalis in the host.

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http://hdl.handle.net/10453/36817