Application of neurophysiological methodology in acupuncture research
- Publication Type:
- Thesis
- Issue Date:
- 2006
Open Access
Copyright Clearance Process
- Recently Added
- In Progress
- Open Access
This item is open access.
Title: Application of neurophysiological methodology in acupuncture research.
Background: Increased popularity and acceptance of acupuncture also increases demand
for scientific evidence of its effectiveness. In order to produce such evidence
acupuncture research borrows methods from other scientific disciplines. Thus it is
essential to validate the usefulness of such methods when they are employed specifically
in physiological research of the acupuncture phenomenon. Confirmation of
physiological changes due to acupuncture stimulation could increase engagement in
acupuncture research and make direct comparisons with biomedical research possible.
Objectives: The main objective was to evaluate the usefulness of non-invasive
neurophysiological methods in detecting physiological changes in response to manual
acupuncture. It was not the purpose of this study to find a proof that acupuncture works
or how it works, but to confirm that acupuncture can be successfully researched using
objective, non-invasive neurophysiological methods.
Method: A rigorously designed, randomised controlled trial (RCT) was used to evaluate
the effectiveness of some of the neurophysiological research methods. Three-point
criteria was set up. Firstly, to investigate if manual stimulation of the acupuncture points
can induce physiological changes in healthy subjects that are strong enough to be
detected by non-invasive testing methods. Secondly, to investigate if physiological
changes induced by acupuncture stimulation form a pattern, which is unique for every
individual acupuncture point being tested. And thirdly, to investigate if physiological
changes induced by acupuncture are more pronounced in subjects initially showing
stronger signs of subjective / objective stress. Overall, sixty healthy female subjects
were recruited for the study, and one hundred and twenty experimental sessions were
completed in order to collect the data. Objective measurements by means of
multichannel computerised recording were used to capture concurrently occurring
physiological events.
Results: Acupuncture treatment used in group one (T1) using acupuncture point LU7
and in group three (T3) using acupuncture points LU7 and KD6 promoted deeper
relaxation and light sleep in subjects showing higher levels of anxiety. In contrast,
subjects in group two (T2) where only acupuncture point KD6 was stimulated, did not
relax or easily fall into light sleep during the 40 minutes post-treatment. Usually Theta
and Alpha “relaxation” waves were changed. Of the two treatment protocols T1 and T3,
the T1 showed the strongest differences. This can be interpreted as indicating that
acupuncture point LU7 promotes relaxation and light sleep. Stimulation of acupoint
KD6 counteracted the relaxation effect promoted by acupoint LU7. Data from the
experiments clearly showed that stimulation of two acupuncture points LU7 and KD6
did not influence heart beat in a strong way. The treatment effect was only detected in
group T2 (acupoint KD6) where an increase in the sympathetic regulation may indicate
that KD6 has a balancing and energising effect on heart rhythm. The respiration rate and
respiration amplitude seems to be unchanged by acupuncture stimulation. There were no
statistically significant differences in body temperature before and after the experiment.
Systolic and diastolic blood pressure remained very stable and was not influenced by
acupuncture stimulation of the two acupoints LU7 and KD6. Analysis of the
electrodermal activity (EDA) data confirmed that changes in skin potential are very
sensitive and specific to needle insertion and needle removal, but not specific to the site
of the insertion. Physiological habituation seems to play significant role in diminishing
the EDA response to manipulation of the acupuncture needle.
Conclusion: Insertion of a single needle invokes physiological changes that can be
detected by neurophysiological methods. Certain acupoint-specific patterns of change
can be seen in multichannel recordings. Results suggest that neurophysiological
methods are appropriate for, and can be adopted in acupuncture studies. Furthermore,
recommendations regarding research designs were formulated. They include a strong
need for a separate control group in acupuncture studies and highlight the importance of
continuous monitoring of multiple physiological parameters during experiments of that
type. New methods of data analysis based on neural networks gave very promising
results that may rival traditional statistical linear models. Overall the outcome of this
project may serve as a call for standardisation of neurophysiological research protocols
in acupuncture laboratories.
Please use this identifier to cite or link to this item: