Visualising mouse neuroanatomy and function by metal distribution using laser ablation-inductively coupled plasma-mass spectrometry imaging

Paul, B; Hare, DJ; Bishop, DP; Paton, C; Nguyen, VT; Cole, N; Niedwiecki, MM; Andreozzi, E; Vais, A; Billings, JL; Bray, L; Bush, AI; McColl, G; Roberts, BR; Adlard, PA; Finkelstein, DI; Hellstrom, J; Hergt, JM; Woodhead, JD; Doble, PA

Visualising mouse neuroanatomy and function by metal distribution using laser ablation-inductively coupled plasma-mass spectrometry imaging

Paul, B Hare, DJ

Bishop, DP

Paton, C Nguyen, VT Cole, N

Niedwiecki, MM Andreozzi, E Vais, A Billings, JL Bray, L Bush, AI McColl, G Roberts, BR Adlard, PA Finkelstein, DI Hellstrom, J Hergt, JM Woodhead, JD Doble, PA

Permalink

Publication Type:: Journal Article
Citation:: Chemical Science, 2015, 6 (10), pp. 5383 - 5393
Issue Date:: 2015-07-27

Open Access

Copyright Clearance Process

Recently Added
In Progress
Open Access

This item is open access.

Adobe PDF

Download Published VersionAdobe PDF (2.21 MB)

View on publisher's site

View statistics

Full metadata record

Field	Value	Language
dc.contributor.author	Paul, B	en_US
dc.contributor.author	Hare, DJ https://orcid.org/0000-0002-5922-7643	en_US
dc.contributor.author	Bishop, DP https://orcid.org/0000-0002-6533-4410	en_US
dc.contributor.author	Paton, C	en_US
dc.contributor.author	Nguyen, VT	en_US
dc.contributor.author	Cole, N https://orcid.org/0000-0002-9866-4694	en_US
dc.contributor.author	Niedwiecki, MM	en_US
dc.contributor.author	Andreozzi, E	en_US
dc.contributor.author	Vais, A	en_US
dc.contributor.author	Billings, JL	en_US
dc.contributor.author	Bray, L	en_US
dc.contributor.author	Bush, AI	en_US
dc.contributor.author	McColl, G	en_US
dc.contributor.author	Roberts, BR	en_US
dc.contributor.author	Adlard, PA	en_US
dc.contributor.author	Finkelstein, DI	en_US
dc.contributor.author	Hellstrom, J	en_US
dc.contributor.author	Hergt, JM	en_US
dc.contributor.author	Woodhead, JD	en_US
dc.contributor.author	Doble, PA https://orcid.org/0000-0002-8472-1301	en_US
dc.date.available	2015-07-27	en_US
dc.date.issued	2015-07-27	en_US
dc.identifier.citation	Chemical Science, 2015, 6 (10), pp. 5383 - 5393	en_US
dc.identifier.issn	2041-6520	en_US
dc.identifier.uri	http://hdl.handle.net/10453/37800
dc.description.abstract	© The Royal Society of Chemistry 2015. Metals have a number of important roles within the brain. We used laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) to map the three-dimensional concentrations and distributions of transition metals, in particular iron (Fe), copper (Cu) and zinc (Zn) within the murine brain. LA-ICP-MS is one of the leading analytical tools for measuring metals in tissue samples. Here, we present a complete data reduction protocol for measuring metals in biological samples, including the application of a pyramidal voxel registration technique to reproducibly align tissue sections. We used gold (Au) nanoparticle and ytterbium (Yb)-tagged tyrosine hydroxylase antibodies to assess the co-localisation of Fe and dopamine throughout the entire mouse brain. We also examined the natural clustering of metal concentrations within the murine brain to elucidate areas of similar composition. This clustering technique uses a mathematical approach to identify multiple 'elemental clusters', avoiding user bias and showing that metal composition follows a hierarchical organisation of neuroanatomical structures. This work provides new insight into the distinct compartmentalisation of metals in the brain, and presents new avenues of exploration with regard to region-specific, metal-associated neurodegeneration observed in several chronic neurodegenerative diseases.	en_US
dc.relation.ispartof	Chemical Science	en_US
dc.relation.isbasedon	10.1039/c5sc02231b	en_US
dc.title	Visualising mouse neuroanatomy and function by metal distribution using laser ablation-inductively coupled plasma-mass spectrometry imaging	en_US
dc.type	Journal Article
utslib.citation.volume	10	en_US
utslib.citation.volume	6	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	03 Chemical Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Chemistry and Forensic Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CFS - Centre for Forensic Science
utslib.copyright.status	open_access
pubs.issue	10	en_US
pubs.publication-status	Published	en_US
pubs.volume	6	en_US

Abstract:

© The Royal Society of Chemistry 2015. Metals have a number of important roles within the brain. We used laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) to map the three-dimensional concentrations and distributions of transition metals, in particular iron (Fe), copper (Cu) and zinc (Zn) within the murine brain. LA-ICP-MS is one of the leading analytical tools for measuring metals in tissue samples. Here, we present a complete data reduction protocol for measuring metals in biological samples, including the application of a pyramidal voxel registration technique to reproducibly align tissue sections. We used gold (Au) nanoparticle and ytterbium (Yb)-tagged tyrosine hydroxylase antibodies to assess the co-localisation of Fe and dopamine throughout the entire mouse brain. We also examined the natural clustering of metal concentrations within the murine brain to elucidate areas of similar composition. This clustering technique uses a mathematical approach to identify multiple 'elemental clusters', avoiding user bias and showing that metal composition follows a hierarchical organisation of neuroanatomical structures. This work provides new insight into the distinct compartmentalisation of metals in the brain, and presents new avenues of exploration with regard to region-specific, metal-associated neurodegeneration observed in several chronic neurodegenerative diseases.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/37800