Antimony concentrations in nodal tissue can confirm sentinel node identity

Scolyer, RA; Thompson, JF; Li, LXL; Beavis, A; Dawson, M; Doble, P; Soper, R; Uren, RF; Stretch, JR; Sharma, R; McCarthy, SW

Antimony concentrations in nodal tissue can confirm sentinel node identity

Scolyer, RA Thompson, JF Li, LXL Beavis, A

Dawson, M Doble, P

Soper, R Uren, RF Stretch, JR Sharma, R McCarthy, SW

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Publication Type:: Journal Article
Citation:: Modern Pathology, 2004, 17 (10), pp. 1191 - 1197
Issue Date:: 2004-10-01

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Field	Value	Language
dc.contributor.author	Scolyer, RA	en_US
dc.contributor.author	Thompson, JF	en_US
dc.contributor.author	Li, LXL	en_US
dc.contributor.author	Beavis, A https://orcid.org/0000-0001-6259-7699	en_US
dc.contributor.author	Dawson, M	en_US
dc.contributor.author	Doble, P https://orcid.org/0000-0002-8472-1301	en_US
dc.contributor.author	Soper, R	en_US
dc.contributor.author	Uren, RF	en_US
dc.contributor.author	Stretch, JR	en_US
dc.contributor.author	Sharma, R	en_US
dc.contributor.author	McCarthy, SW	en_US
dc.date.issued	2004-10-01	en_US
dc.identifier.citation	Modern Pathology, 2004, 17 (10), pp. 1191 - 1197	en_US
dc.identifier.issn	0893-3952	en_US
dc.identifier.uri	http://hdl.handle.net/10453/4710
dc.description.abstract	The sentinel node biopsy procedure is a highly accurate method of staging patients with cutaneous melanoma and the tumor-harboring status of sentinel nodes is the most important prognostic factor. For the procedure to provide accurate prognostic information, however, it is essential that 'true' sentinel nodes are removed and examined thoroughly. A technique to confirm sentinel node identity may reduce the false-negative rate of the procedure. We have found that antimony (originating from the antimony sulfide colloid used for preoperative lymphoscintigraphy in our institution) can be measured in tissue sections of sentinel nodes using inductively coupled plasma mass spectrometry. The aims of this study were to determine whether antimony concentrations can be used to confirm that removed sentinel nodes are 'true' sentinel nodes and to differentiate sentinel nodes from nonsentinel nodes. In all, 24 patients who had both a tumor-positive sentinel node and a tumor-negative nonsentinel node removed from one regional node field during the same operation, were identified. Tissue sections (50 μm) thick were cut from archival paraffin blocks of each of the sentinel nodes and nonsentinel nodes. Antimony concentrations in the tissue sections were measured using inductively coupled plasma mass spectrometry. The median and mean concentrations of antimony in parts per billion were 0.526 and 1.198, respectively (range 0.020-7.596) in the sentinel nodes, and 0.043 and 0.123 (range 0-0.800) in the nonsentinel nodes (P = 0.004). In four of the 24 pairs, both the presumed sentinel nodes and the nonsentinel nodes had very low antimony levels (less than 0.18 parts per billion), suggesting that nodes designated as sentinel nodes may not have been 'true' sentinel nodes. It is concluded that determination of antimony concentrations within sentinel nodes using the highly sensitive method of inductively coupled plasma mass spectrometry can confirm the identity of sentinel nodes and validate the sentinel node technique.	en_US
dc.relation	http://purl.org/au-research/grants/arc/LE0454207
dc.relation.ispartof	Modern Pathology	en_US
dc.relation.isbasedon	10.1038/modpathol.3800202	en_US
dc.subject.classification	Pathology	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Melanoma	en_US
dc.subject.mesh	Skin Neoplasms	en_US
dc.subject.mesh	Lymphatic Metastasis	en_US
dc.subject.mesh	Antimony	en_US
dc.subject.mesh	Sentinel Lymph Node Biopsy	en_US
dc.subject.mesh	Reproducibility of Results	en_US
dc.subject.mesh	Predictive Value of Tests	en_US
dc.subject.mesh	Radionuclide Imaging	en_US
dc.title	Antimony concentrations in nodal tissue can confirm sentinel node identity	en_US
dc.type	Journal Article
utslib.citation.volume	10	en_US
utslib.citation.volume	17	en_US
utslib.for	1103 Clinical Sciences	en_US
utslib.for	11 Medical and Health Sciences	en_US
dc.location.activity	Gold Coast, Australia
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Chemistry and Forensic Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CFS - Centre for Forensic Science
utslib.copyright.status	closed_access
pubs.issue	10	en_US
pubs.publication-status	Published	en_US
pubs.volume	17	en_US

Abstract:

The sentinel node biopsy procedure is a highly accurate method of staging patients with cutaneous melanoma and the tumor-harboring status of sentinel nodes is the most important prognostic factor. For the procedure to provide accurate prognostic information, however, it is essential that 'true' sentinel nodes are removed and examined thoroughly. A technique to confirm sentinel node identity may reduce the false-negative rate of the procedure. We have found that antimony (originating from the antimony sulfide colloid used for preoperative lymphoscintigraphy in our institution) can be measured in tissue sections of sentinel nodes using inductively coupled plasma mass spectrometry. The aims of this study were to determine whether antimony concentrations can be used to confirm that removed sentinel nodes are 'true' sentinel nodes and to differentiate sentinel nodes from nonsentinel nodes. In all, 24 patients who had both a tumor-positive sentinel node and a tumor-negative nonsentinel node removed from one regional node field during the same operation, were identified. Tissue sections (50 μm) thick were cut from archival paraffin blocks of each of the sentinel nodes and nonsentinel nodes. Antimony concentrations in the tissue sections were measured using inductively coupled plasma mass spectrometry. The median and mean concentrations of antimony in parts per billion were 0.526 and 1.198, respectively (range 0.020-7.596) in the sentinel nodes, and 0.043 and 0.123 (range 0-0.800) in the nonsentinel nodes (P = 0.004). In four of the 24 pairs, both the presumed sentinel nodes and the nonsentinel nodes had very low antimony levels (less than 0.18 parts per billion), suggesting that nodes designated as sentinel nodes may not have been 'true' sentinel nodes. It is concluded that determination of antimony concentrations within sentinel nodes using the highly sensitive method of inductively coupled plasma mass spectrometry can confirm the identity of sentinel nodes and validate the sentinel node technique.

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http://hdl.handle.net/10453/4710