A rapid and sensitive high-performace liquid chromatography-electrospray ionization-triple quadrupole mass spectometry method for the quantitation of oxycodone in human plasma

Preston Publications Inc.
Publication Type:
Journal Article
Journal of Chromatographic Science, 2002, 40 (1), pp. 40 - 44
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A sensitive method for the determination of oxycodone concentrations in plasma by high-performance liquid chromatography (HPLC)electrospray ionizationtriple quadrupole mass spectrometry is described. The method is rugged, reliable, selective, and rapid with a run time of 2 min. One milliliter of plasma is made basic and extracted with 2-mL duplicate portions of 2% isoamyl alcohol in n-butyl chloride. The combined extracts are then evaporated to dryness, reconstituted in 100 µL of the mobile phase (15% methanol85% water containing 0.1% acetic acid), and injected onto the HPLC. The limit of quantitation is 1 ng/mL, and the estimated limit of detection is 33 pg/mL (signal-to-noise = 3). Standard curves are linear over the range of 1 to 100 ng/mL with all correlation coefficient values greater than 0.9989. The method is used to determine the concentration of oxycodone in human plasma following the intravenous infusion of doses ranging from 5 to 15 mg in which the analysis of over 3000 plasma samples is required.
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