Molecular diagnosis of entamoeba species
- Publication Type:
- Thesis
- Issue Date:
- 2007
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01Front.pdf | contents and abstract | 537.82 kB | |||
02Whole.pdf | thesis | 6.55 MB |
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NO FULL TEXT AVAILABLE. Access is restricted indefinitely. ----- Amebiasis, one of the major intestinal diseases of man, is caused by the
protozoan pathogen Entamoeba histolytica. It is the second leading cause of
death from parasitic disease worldwide. Although E. histolytica is recognised as
a pathogen, the ability of the other Entamoeba species to cause disease is
unclear. However, recent studies highlight the recovery of Entamoeba dispar and
Entamoeba moshkovskii from patients with gastrointestinal symptoms.
Traditionally, E. histolytica was diagnosed by microscopy of stool samples
however, with the discovery of two other morphologically similar species (E.
dispar and E. moshkovskii), microscopy can no longer be relied upon to diagnose
amebiasis. In this study a total of 5921 samples were tested over a period
between January 2003- June 2006, which were submitted to the Department of
Microbiology at St.Vincent's hospital Sydney, Australia. One hundred and
seventy seven samples (2.9%) were microscopically positive for cysts or
trophozoites of E complex (E.histolytica, E.dispar, E. moshkovskii), either singly
or in combination with other human protozoan parasites. In addition, stool
samples collected from patients microscopically negative for parasitic cyst or
ova (51) and healthy controls (51) (control group) were also tested. PCR
targeting the 18S ribosomal rDNA was performed on stool samples and the
amplicons were confirmed using sequencing. The PCR showed a specificity of
100% and a sensitivity of 81%. A prevalence of 5.6 % was observed for E.
histolytica and a higher prevalence of E. dispar (70.7%) and E. moshkovskii
(61.8%) was found. The patients infected with only E. dispar (5 patients), E.
moshkovskii (3 patients) and with a mixture of both (7 patients), and the absence
of other protozoan parasites, viral and bacterial pathogens indicate the potential
of these organisms in causing diarrhoea and abdominal pain.
DNA of E. moshkovskii was not detected in the stool samples collected
from the healthy controls (control group). These results further suggest that E.
moshkovskii may not simply be a commensal of the human gastrointestinal
tract and provides additional evidence for E. moshkovskii as a cause of diarrhea
and other gastrointestinal disorders. The findings of this study raises concern
that E. moshkovskii could be a potential pathogen thereby suggesting a review
of the pathogenic status of this parasite is necessary. Further studies are needed
to determine the role of different strains of E. moshkovskii in causing diarrheal
diseases in our population.
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