Challenge of New Zealand Greenshell™ mussel Perna canaliculus larvae using two Vibrio pathogens: A hatchery study

Kesarcodi-Watson, A; Kaspar, H; Lategan, MJ; Gibson, LF

Challenge of New Zealand Greenshell™ mussel Perna canaliculus larvae using two Vibrio pathogens: A hatchery study

Kesarcodi-Watson, A Kaspar, H Lategan, MJ Gibson, LF

Permalink

Publication Type:: Journal Article
Citation:: Diseases of Aquatic Organisms, 2009, 86 (1), pp. 15 - 20
Issue Date:: 2009-10-01

Closed Access

	Filename	Description	Size
	2008007751OK.pdf		353.74 kB	Adobe PDF	View/Open

Copyright Clearance Process

Recently Added
In Progress
Closed Access

This item is closed access and not available.

Full metadata record

Field	Value	Language
dc.contributor.author	Kesarcodi-Watson, A	en_US
dc.contributor.author	Kaspar, H	en_US
dc.contributor.author	Lategan, MJ	en_US
dc.contributor.author	Gibson, LF	en_US
dc.date.issued	2009-10-01	en_US
dc.identifier.citation	Diseases of Aquatic Organisms, 2009, 86 (1), pp. 15 - 20	en_US
dc.identifier.issn	0177-5103	en_US
dc.identifier.uri	http://hdl.handle.net/10453/8854
dc.description.abstract	Bacterial diseases ra large problem in aquaculture hatcheries. The successful design and implementation of protective measures in the hatchery depends on an understanding of the dynamics of the infection process. Developing an in situ experimental protocol for pathogen challenge is therefore of paramount importance. Here, we demonstrated the minimum effective pathogenic dose (MEPD) of Vibrio splendidus (105 CFU ml-1) and a Vibrio coralliilyticus/neptunius-like isolate, Vibrio sp. DO1 (106 CFU ml-1), for New Zealand Greenshell™ mussel (GSM, Perna canaliculus) larvae during hatchery production. In a flow-through water hatchery system, larvae given 1 to 2 h of static water exposure to these pathogen doses showed respective average cumulative mortalities of 58 and 69% on the fourth day following pathogen exposure. After the 1 to 2 h static exposure, larvae were returned to flow-through water. Larvae exposed to a dosage one order of magnitude greater than the MEPD had higher mortalities of 73 and 96% for V. splendidus and Vibrio sp. DO1 respectively. These 4 levels of mortality were significantly greater than those of the non-exposed control larvae which respectively averaged 23 and 35% in experiments involving V. splendidus and Vibrio sp. DO1. Experiments were repeated 4 times to confirm reproducibility. After pathogen exposure, pathogens were detected in the larvae and tank water of treatments with dosages of ≥ 105 CFU ml-5 (V. splendidus) and 106 CFU ml-1 (Vibrio sp. DO1), but not in treatments with lower pathogen dosages. The challenge protocols are reproducible and provide an opportunity to assess measures for the protection of GSM larvae against infection in the hatchery environment. © Inter-Research 2009.	en_US
dc.relation.ispartof	Diseases of Aquatic Organisms	en_US
dc.relation.isbasedon	10.3354/dao02100	en_US
dc.subject.classification	Fisheries	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Vibrio	en_US
dc.subject.mesh	Survival Analysis	en_US
dc.subject.mesh	Seawater	en_US
dc.subject.mesh	Larva	en_US
dc.subject.mesh	Time Factors	en_US
dc.subject.mesh	Aquaculture	en_US
dc.subject.mesh	New Zealand	en_US
dc.subject.mesh	Perna	en_US
dc.subject.mesh	Host-Pathogen Interactions	en_US
dc.title	Challenge of New Zealand Greenshell™ mussel Perna canaliculus larvae using two Vibrio pathogens: A hatchery study	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.citation.volume	86	en_US
utslib.for	070404 Fish Pests and Diseases	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	07 Agricultural and Veterinary Sciences	en_US
dc.location.activity	ISI:000270802000003	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Medical and Molecular Sciences
utslib.copyright.status	closed_access
pubs.issue	1	en_US
pubs.publication-status	Published	en_US
pubs.volume	86	en_US

Abstract:

Bacterial diseases ra large problem in aquaculture hatcheries. The successful design and implementation of protective measures in the hatchery depends on an understanding of the dynamics of the infection process. Developing an in situ experimental protocol for pathogen challenge is therefore of paramount importance. Here, we demonstrated the minimum effective pathogenic dose (MEPD) of Vibrio splendidus (105 CFU ml-1) and a Vibrio coralliilyticus/neptunius-like isolate, Vibrio sp. DO1 (106 CFU ml-1), for New Zealand Greenshell™ mussel (GSM, Perna canaliculus) larvae during hatchery production. In a flow-through water hatchery system, larvae given 1 to 2 h of static water exposure to these pathogen doses showed respective average cumulative mortalities of 58 and 69% on the fourth day following pathogen exposure. After the 1 to 2 h static exposure, larvae were returned to flow-through water. Larvae exposed to a dosage one order of magnitude greater than the MEPD had higher mortalities of 73 and 96% for V. splendidus and Vibrio sp. DO1 respectively. These 4 levels of mortality were significantly greater than those of the non-exposed control larvae which respectively averaged 23 and 35% in experiments involving V. splendidus and Vibrio sp. DO1. Experiments were repeated 4 times to confirm reproducibility. After pathogen exposure, pathogens were detected in the larvae and tank water of treatments with dosages of ≥ 105 CFU ml-5 (V. splendidus) and 106 CFU ml-1 (Vibrio sp. DO1), but not in treatments with lower pathogen dosages. The challenge protocols are reproducible and provide an opportunity to assess measures for the protection of GSM larvae against infection in the hatchery environment. © Inter-Research 2009.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/8854