Confirmation of sentinel lymph node identity by analysis of fine-needle biopsy samples using inductively coupled plasma-mass spectrometry

Beavis, A; Dawson, M; Doble, P; Scolyer, RA; Bourne, R; Li, LXL; Murali, R; Stretch, JR; Lean, CL; Uren, RF; Thompson, JF

Confirmation of sentinel lymph node identity by analysis of fine-needle biopsy samples using inductively coupled plasma-mass spectrometry

Beavis, A

Dawson, M Doble, P

Scolyer, RA Bourne, R Li, LXL Murali, R Stretch, JR Lean, CL Uren, RF Thompson, JF

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Publication Type:: Journal Article
Citation:: Annals of Surgical Oncology, 2008, 15 (3), pp. 934 - 940
Issue Date:: 2008-03-01

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Field	Value	Language
dc.contributor.author	Beavis, A https://orcid.org/0000-0001-6259-7699	en_US
dc.contributor.author	Dawson, M	en_US
dc.contributor.author	Doble, P https://orcid.org/0000-0002-8472-1301	en_US
dc.contributor.author	Scolyer, RA	en_US
dc.contributor.author	Bourne, R	en_US
dc.contributor.author	Li, LXL	en_US
dc.contributor.author	Murali, R	en_US
dc.contributor.author	Stretch, JR	en_US
dc.contributor.author	Lean, CL	en_US
dc.contributor.author	Uren, RF	en_US
dc.contributor.author	Thompson, JF	en_US
dc.date.available	2007-10-15	en_US
dc.date.issued	2008-03-01	en_US
dc.identifier.citation	Annals of Surgical Oncology, 2008, 15 (3), pp. 934 - 940	en_US
dc.identifier.issn	1068-9265	en_US
dc.identifier.uri	http://hdl.handle.net/10453/9600
dc.description.abstract	Background: The sentinel lymph node (SLN) biopsy technique is a reliable means of determining the tumor-harboring status of regional lymph nodes in melanoma patients. When technetium 99 m-labeled antimony trisulfide colloid (99 mTc-Sb2S3) particles are used to perform preoperative lymphoscintigraphy for SLN identification, they are retained in the SLN but are absent or present in only tiny amounts in non-SLNs. The present study investigated the potential for a novel means of assessing the accuracy of surgical identification of SLNs. This involved the use of inductively coupled plasma-mass spectrometry (ICP-MS) to analyze antimony concentrations in fine-needle biopsy (FNB) samples from surgically procured lymph nodes. Methods: A total of 47 FNB samples from surgically excised lymph nodes (32 SLNs and 15 non-SLNs) were collected. The SLNs were localized by preoperative lymphoscintigraphy that used 99 mTc-Sb2S3, blue dye, and gamma probe techniques. The concentrations of antimony were measured in the FNB samples by ICP-MS. Results: The mean and median antimony concentrations (in parts per billion) were .898 and .451 in the SLNs, and .015 and .068 in the non-SLNs, the differences being highly statistically significant (P < .00005). Conclusions: Our results show that ICP-MS analysis of antimony concentrations in FNB specimens from lymph nodes can accurately confirm the identity of SLNs. Used in conjunction with techniques such as proton magnetic resonance spectroscopy for the nonsurgical evaluation of SLNs, ICP-MS analysis of antimony concentrations in FNB samples could potentially serve as a minimally invasive alternative to surgery and histopathologic evaluation to objectively classify a given node as sentinel or nonsentinel and determine its tumor-harboring status. © 2007 The Author(s).	en_US
dc.relation.ispartof	Annals of Surgical Oncology	en_US
dc.relation.isbasedon	10.1245/s10434-007-9693-0	en_US
dc.subject.classification	Oncology & Carcinogenesis	en_US
dc.subject.mesh	Lymph Nodes	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Melanoma	en_US
dc.subject.mesh	Skin Neoplasms	en_US
dc.subject.mesh	Antimony	en_US
dc.subject.mesh	Radiopharmaceuticals	en_US
dc.subject.mesh	Biopsy, Fine-Needle	en_US
dc.subject.mesh	Sentinel Lymph Node Biopsy	en_US
dc.subject.mesh	Mass Spectrometry	en_US
dc.title	Confirmation of sentinel lymph node identity by analysis of fine-needle biopsy samples using inductively coupled plasma-mass spectrometry	en_US
dc.type	Journal Article
utslib.citation.volume	3	en_US
utslib.citation.volume	15	en_US
utslib.for	1112 Oncology and Carcinogenesis	en_US
dc.location.activity	ISI:000252990700036	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Chemistry and Forensic Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CFS - Centre for Forensic Science
utslib.copyright.status	closed_access
pubs.issue	3	en_US
pubs.publication-status	Published	en_US
pubs.volume	15	en_US

Abstract:

Background: The sentinel lymph node (SLN) biopsy technique is a reliable means of determining the tumor-harboring status of regional lymph nodes in melanoma patients. When technetium 99 m-labeled antimony trisulfide colloid (99 mTc-Sb2S3) particles are used to perform preoperative lymphoscintigraphy for SLN identification, they are retained in the SLN but are absent or present in only tiny amounts in non-SLNs. The present study investigated the potential for a novel means of assessing the accuracy of surgical identification of SLNs. This involved the use of inductively coupled plasma-mass spectrometry (ICP-MS) to analyze antimony concentrations in fine-needle biopsy (FNB) samples from surgically procured lymph nodes. Methods: A total of 47 FNB samples from surgically excised lymph nodes (32 SLNs and 15 non-SLNs) were collected. The SLNs were localized by preoperative lymphoscintigraphy that used 99 mTc-Sb2S3, blue dye, and gamma probe techniques. The concentrations of antimony were measured in the FNB samples by ICP-MS. Results: The mean and median antimony concentrations (in parts per billion) were .898 and .451 in the SLNs, and .015 and .068 in the non-SLNs, the differences being highly statistically significant (P < .00005). Conclusions: Our results show that ICP-MS analysis of antimony concentrations in FNB specimens from lymph nodes can accurately confirm the identity of SLNs. Used in conjunction with techniques such as proton magnetic resonance spectroscopy for the nonsurgical evaluation of SLNs, ICP-MS analysis of antimony concentrations in FNB samples could potentially serve as a minimally invasive alternative to surgery and histopathologic evaluation to objectively classify a given node as sentinel or nonsentinel and determine its tumor-harboring status. © 2007 The Author(s).

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