Direct and indirect methods for determining plasma volume during thermoneutral and cold-water immersion.

Gordon, CJ; Fogarty, AL; Greenleaf, JE; Taylor, NAS; Stocks, JM

Direct and indirect methods for determining plasma volume during thermoneutral and cold-water immersion.

Gordon, CJ Fogarty, AL Greenleaf, JE Taylor, NAS Stocks, JM

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Publication Type:: Journal Article
Citation:: Eur J Appl Physiol, 2003, 89 (5), pp. 471 - 474
Issue Date:: 2003-06

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Field	Value	Language
dc.contributor.author	Gordon, CJ	en_US
dc.contributor.author	Fogarty, AL	en_US
dc.contributor.author	Greenleaf, JE	en_US
dc.contributor.author	Taylor, NAS	en_US
dc.contributor.author	Stocks, JM	en_US
dc.date.available	2003-02-17	en_US
dc.date.issued	2003-06	en_US
dc.identifier.citation	Eur J Appl Physiol, 2003, 89 (5), pp. 471 - 474	en_US
dc.identifier.issn	1439-6319	en_US
dc.identifier.uri	http://hdl.handle.net/10453/9691
dc.description.abstract	Plasma volume (PV), determined indirectly from changes in haematocrit (Hct) and haemoglobin concentration ([Hb]), underestimates the absolute PV change (Evans blue dye) during thermoneutral immersion. Since PV changes during cold-water immersion have only been determined indirectly, we hypothesised that a similar underestimation may occur. Therefore, we compared the indirectly-measured PV with a direct-tracer dilution method (Evans blue dye column elution) in seven healthy males, during three, 60-min exposures: air (control; 21.2 degrees C), thermoneutral immersion (34.5 degrees C) and cold-water immersion (18.6 degrees C). During thermoneutral immersion, the directly-measured PV increased by 16.2 (1.4)% (P<0.05) and the indirectly-measured by 8.5 (0.8)% (P<0.05), with the latter underestimating the former by 43 (9.1)% (P<0.05). During cold immersion, the direct PV decreased by 17.9 (3.0)% (P<0.05) and the indirect by 8.0 (1.2)% (P<0.05), with the latter representing a 52 (6.8)% (P<0.05) underestimation of the direct PV change. Directionally-equivalent underestimations of PV change occur when using the indirect method during both thermoneutral and cold-water immersion. The assumptions inherent in the indirect method (constant F-cell ratio) appear to be violated during water immersion.	en_US
dc.language	eng	en_US
dc.relation.ispartof	Eur J Appl Physiol	en_US
dc.relation.isbasedon	10.1007/s00421-003-0823-5	en_US
dc.subject.classification	Sport Sciences	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Evans Blue	en_US
dc.subject.mesh	Hemoglobins	en_US
dc.subject.mesh	Hematocrit	en_US
dc.subject.mesh	Sensitivity and Specificity	en_US
dc.subject.mesh	Reproducibility of Results	en_US
dc.subject.mesh	Immersion	en_US
dc.subject.mesh	Indicator Dilution Techniques	en_US
dc.subject.mesh	Body Temperature Regulation	en_US
dc.subject.mesh	Plasma Volume	en_US
dc.subject.mesh	Adult	en_US
dc.subject.mesh	Male	en_US
dc.subject.mesh	Statistics as Topic	en_US
dc.subject.mesh	Cold Temperature	en_US
dc.title	Direct and indirect methods for determining plasma volume during thermoneutral and cold-water immersion.	en_US
dc.type	Journal Article
utslib.citation.volume	5	en_US
utslib.citation.volume	89	en_US
utslib.location.activity	Germany	en_US
utslib.for	1106 Human Movement and Sports Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Health
utslib.copyright.status	closed_access
pubs.issue	5	en_US
pubs.publication-status	Published	en_US
pubs.volume	89	en_US

Abstract:

Plasma volume (PV), determined indirectly from changes in haematocrit (Hct) and haemoglobin concentration ([Hb]), underestimates the absolute PV change (Evans blue dye) during thermoneutral immersion. Since PV changes during cold-water immersion have only been determined indirectly, we hypothesised that a similar underestimation may occur. Therefore, we compared the indirectly-measured PV with a direct-tracer dilution method (Evans blue dye column elution) in seven healthy males, during three, 60-min exposures: air (control; 21.2 degrees C), thermoneutral immersion (34.5 degrees C) and cold-water immersion (18.6 degrees C). During thermoneutral immersion, the directly-measured PV increased by 16.2 (1.4)% (P<0.05) and the indirectly-measured by 8.5 (0.8)% (P<0.05), with the latter underestimating the former by 43 (9.1)% (P<0.05). During cold immersion, the direct PV decreased by 17.9 (3.0)% (P<0.05) and the indirect by 8.0 (1.2)% (P<0.05), with the latter representing a 52 (6.8)% (P<0.05) underestimation of the direct PV change. Directionally-equivalent underestimations of PV change occur when using the indirect method during both thermoneutral and cold-water immersion. The assumptions inherent in the indirect method (constant F-cell ratio) appear to be violated during water immersion.

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http://hdl.handle.net/10453/9691