Enhanced invasive properties exhibited by smooth muscle cells are associated with elevated production of MMP-2 in patients with aortic aneurysms

Goodall, S; Porter, KE; Bell, PR; Thompson, MM

Enhanced invasive properties exhibited by smooth muscle cells are associated with elevated production of MMP-2 in patients with aortic aneurysms

Goodall, S

Porter, KE Bell, PR Thompson, MM

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Publication Type:: Journal Article
Citation:: European Journal of Vascular and Endovascular Surgery, 2002, 24 (1), pp. 72 - 80
Issue Date:: 2002-01-01

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Field	Value	Language
dc.contributor.author	Goodall, S https://orcid.org/0000-0001-6611-6565	en_US
dc.contributor.author	Porter, KE	en_US
dc.contributor.author	Bell, PR	en_US
dc.contributor.author	Thompson, MM	en_US
dc.date.issued	2002-01-01	en_US
dc.identifier.citation	European Journal of Vascular and Endovascular Surgery, 2002, 24 (1), pp. 72 - 80	en_US
dc.identifier.issn	1078-5884	en_US
dc.identifier.uri	http://hdl.handle.net/10453/9986
dc.description.abstract	Background: abdominal aortic aneurysms (AAA) are associated with excessive vascular matrix remodelling. Recent findings suggest a systemic overproduction of matrix metalloproteinases-2 (MMP-2) by vascular smooth muscle cells (SMC) may be pivotal aetiologically. SMC migration is facilitated by MMP mediated proteolysis of the basement membrane and extracellular matrix. Our aim was to see if enhanced MMP-2 production by these SMC exhibit increased invasion, in an in vitro model of migration. Method: SMC were derived from inferior mesenteric vein (IMV) harvested from patients undergoing aneurysm repair (n = 6) or colectomy for diverticulosis (n = 6, control). Using a modified Boy den chamber chemotaxis was measured towards platelet derived growth factor (PDGF) and foetal calf serum (FCS) and invasion through a Matrigel layer. MMP-2 production was quantified by ELISA and gelatin zymography. Results: chemoattractant studies demonstrated no difference in the effect of PDGF or FCS between the two populations of SMC. However, invasive studies demonstrated a significant increase in the number of migrating SMC isolated from IMV of AAA patients. Analysis of culture media extracts revealed that this difference was associated with a significant increase in production of MMP-2. Conclusion: SMC derived from patients with AAA demonstrate increased invasive properties when compared to a control group. Increased migration appears to be due to overproduction of MMP-2. The enhanced migratory potential of these SMC may lead to extracellular matrix remodelling and subsequent medial disruption demonstrated in the aneurysmal aorta. These data further support evidence of the proteolytic role of MMP-2 in cell migration. © 2002 Elsevier Science Ltd. All rights reserved.	en_US
dc.relation.ispartof	European Journal of Vascular and Endovascular Surgery	en_US
dc.relation.isbasedon	10.1053/ejvs.2002.1675	en_US
dc.subject.classification	Cardiovascular System & Hematology	en_US
dc.subject.mesh	Muscle, Smooth, Vascular	en_US
dc.subject.mesh	Mesenteric Veins	en_US
dc.subject.mesh	Cells, Cultured	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Aortic Aneurysm, Abdominal	en_US
dc.subject.mesh	Collagen	en_US
dc.subject.mesh	Proteoglycans	en_US
dc.subject.mesh	Platelet-Derived Growth Factor	en_US
dc.subject.mesh	Laminin	en_US
dc.subject.mesh	Biocompatible Materials	en_US
dc.subject.mesh	Drug Combinations	en_US
dc.subject.mesh	Chemotaxis	en_US
dc.subject.mesh	Matrix Metalloproteinase 2	en_US
dc.title	Enhanced invasive properties exhibited by smooth muscle cells are associated with elevated production of MMP-2 in patients with aortic aneurysms	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.citation.volume	24	en_US
utslib.for	111799 Public Health and Health Services not elsewhere classified	en_US
utslib.for	140208 Health Economics	en_US
utslib.for	1102 Cardiorespiratory Medicine and Haematology	en_US
utslib.for	1103 Clinical Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Business
pubs.organisational-group	/University of Technology Sydney/Strength - CHERE - Centre for Health Economics Research and Evaluation
utslib.copyright.status	closed_access
pubs.issue	1	en_US
pubs.publication-status	Published	en_US
pubs.volume	24	en_US

Abstract:

Background: abdominal aortic aneurysms (AAA) are associated with excessive vascular matrix remodelling. Recent findings suggest a systemic overproduction of matrix metalloproteinases-2 (MMP-2) by vascular smooth muscle cells (SMC) may be pivotal aetiologically. SMC migration is facilitated by MMP mediated proteolysis of the basement membrane and extracellular matrix. Our aim was to see if enhanced MMP-2 production by these SMC exhibit increased invasion, in an in vitro model of migration. Method: SMC were derived from inferior mesenteric vein (IMV) harvested from patients undergoing aneurysm repair (n = 6) or colectomy for diverticulosis (n = 6, control). Using a modified Boy den chamber chemotaxis was measured towards platelet derived growth factor (PDGF) and foetal calf serum (FCS) and invasion through a Matrigel layer. MMP-2 production was quantified by ELISA and gelatin zymography. Results: chemoattractant studies demonstrated no difference in the effect of PDGF or FCS between the two populations of SMC. However, invasive studies demonstrated a significant increase in the number of migrating SMC isolated from IMV of AAA patients. Analysis of culture media extracts revealed that this difference was associated with a significant increase in production of MMP-2. Conclusion: SMC derived from patients with AAA demonstrate increased invasive properties when compared to a control group. Increased migration appears to be due to overproduction of MMP-2. The enhanced migratory potential of these SMC may lead to extracellular matrix remodelling and subsequent medial disruption demonstrated in the aneurysmal aorta. These data further support evidence of the proteolytic role of MMP-2 in cell migration. © 2002 Elsevier Science Ltd. All rights reserved.

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http://hdl.handle.net/10453/9986