Development of a whole cell biosensor for the detection of inorganic mercury

Mahbub, K; Krishnan, K; Naidu, R; Megharaj, M

Development of a whole cell biosensor for the detection of inorganic mercury

Mahbub, K

Krishnan, K Naidu, R Megharaj, M

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Publication Type:: Journal Article
Citation:: Environmental Technology and Innovation, 2017, 8 pp. 64 - 70
Issue Date:: 2017-11-01

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Field	Value	Language
dc.contributor.author	Mahbub, K https://orcid.org/0000-0001-7086-9965	en_US
dc.contributor.author	Krishnan, K	en_US
dc.contributor.author	Naidu, R	en_US
dc.contributor.author	Megharaj, M	en_US
dc.date.available	2020-05-25T19:16:57Z
dc.date.issued	2017-11-01	en_US
dc.identifier.citation	Environmental Technology and Innovation, 2017, 8 pp. 64 - 70	en_US
dc.identifier.uri	http://hdl.handle.net/10453/104587
dc.description.abstract	© 2017 Elsevier B.V. A whole cell bacterial biosensor was developed by modification of chromosomal DNA of an environmental bacterial isolate Sphingobium SA2. The sensing element contained green fluorescence protein gene gfp fused to short segment of merA gene of Sphingobium SA2. The sensing element was introduced into electro-competent cells of Sphingobium SA2, where it integrated into the bacterial DNA due to homologous recombination. The transformed cells were able to produce green fluorescence in 5 h in the presence of nano-molar concentrations of mercury. A linear positive correlation was observed between 0–40 nm Hg and fluorescence intensity.	en_US
dc.relation.ispartof	Environmental Technology and Innovation	en_US
dc.relation.isbasedon	10.1016/j.eti.2017.04.003	en_US
dc.title	Development of a whole cell biosensor for the detection of inorganic mercury	en_US
dc.type	Journal Article
utslib.citation.volume	8	en_US
utslib.for	090703 Environmental Technologies	en_US
utslib.for	0502 Environmental Science and Management	en_US
utslib.for	0907 Environmental Engineering	en_US
utslib.for	1002 Environmental Biotechnology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	open_access
pubs.publication-status	Published	en_US
pubs.volume	8	en_US

Abstract:

© 2017 Elsevier B.V. A whole cell bacterial biosensor was developed by modification of chromosomal DNA of an environmental bacterial isolate Sphingobium SA2. The sensing element contained green fluorescence protein gene gfp fused to short segment of merA gene of Sphingobium SA2. The sensing element was introduced into electro-competent cells of Sphingobium SA2, where it integrated into the bacterial DNA due to homologous recombination. The transformed cells were able to produce green fluorescence in 5 h in the presence of nano-molar concentrations of mercury. A linear positive correlation was observed between 0–40 nm Hg and fluorescence intensity.

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http://hdl.handle.net/10453/104587