Comparative proteomic analysis of two pathogenic Tritrichomonas foetus genotypes: there is more to the proteome than meets the eye

Stroud, LJ; Šlapeta, J; Padula, MP; Druery, D; Tsiotsioras, G; Coorssen, JR; Stack, CM

Comparative proteomic analysis of two pathogenic Tritrichomonas foetus genotypes: there is more to the proteome than meets the eye

Stroud, LJ Šlapeta, J Padula, MP

Druery, D Tsiotsioras, G Coorssen, JR Stack, CM

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Publication Type:: Journal Article
Citation:: International Journal for Parasitology, 2017, 47 (4), pp. 203 - 213
Issue Date:: 2017-03-01

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Field	Value	Language
dc.contributor.author	Stroud, LJ	en_US
dc.contributor.author	Šlapeta, J	en_US
dc.contributor.author	Padula, MP https://orcid.org/0000-0002-8283-0643	en_US
dc.contributor.author	Druery, D	en_US
dc.contributor.author	Tsiotsioras, G	en_US
dc.contributor.author	Coorssen, JR	en_US
dc.contributor.author	Stack, CM	en_US
dc.date.available	2016-11-14	en_US
dc.date.issued	2017-03-01	en_US
dc.identifier.citation	International Journal for Parasitology, 2017, 47 (4), pp. 203 - 213	en_US
dc.identifier.issn	0020-7519	en_US
dc.identifier.uri	http://hdl.handle.net/10453/109353
dc.description.abstract	© 2017 Australian Society for Parasitology Certain clinical isolates of Tritrichomonas foetus infect the urogenital tract of cattle while others infect the gastrointestinal tract of cats. Previous studies have identified subtle genetic differences between these isolates with the term “genotype” adopted to reflect host origin. The aim of this work was to seek evidence of host-specific adaptation and to clarify the relationship between T. foetus genotypes. To do this we characterised the proteomes of both genotypes using two-dimensional gel electrophoresis (2DE) coupled with LC-MS/MS. Our comparative analysis of the data revealed that both genotypes exhibited largely similar proteoform profiles; however differentiation was possible with 24 spots identified as having a four-fold or greater change. Deeper analysis using 2DE zymography and protease-specific fluorogenic substrates revealed marked differences in cysteine protease (CP) expression profiles between the two genotypes. These variances in CP activities could also account for the pathogenic and histopathological differences previously observed between T. foetus genotypes in cross-infection studies. Our findings highlight the importance of CPs as major determinants of parasite virulence and provide a foundation for future host-parasite interaction studies, with direct implications for the development of vaccines or drugs targeting T. foetus.	en_US
dc.relation.ispartof	International Journal for Parasitology	en_US
dc.relation.isbasedon	10.1016/j.ijpara.2016.11.004	en_US
dc.subject.classification	Mycology & Parasitology	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Cattle	en_US
dc.subject.mesh	Cats	en_US
dc.subject.mesh	Tritrichomonas foetus	en_US
dc.subject.mesh	Protozoan Infections	en_US
dc.subject.mesh	Cat Diseases	en_US
dc.subject.mesh	Cattle Diseases	en_US
dc.subject.mesh	Proteome	en_US
dc.subject.mesh	Protozoan Proteins	en_US
dc.subject.mesh	Chromatography, Liquid	en_US
dc.subject.mesh	Electrophoresis, Gel, Two-Dimensional	en_US
dc.subject.mesh	Proteomics	en_US
dc.subject.mesh	Genotype	en_US
dc.subject.mesh	Tandem Mass Spectrometry	en_US
dc.subject.mesh	Cysteine Proteases	en_US
dc.title	Comparative proteomic analysis of two pathogenic Tritrichomonas foetus genotypes: there is more to the proteome than meets the eye	en_US
dc.type	Journal Article
utslib.citation.volume	4	en_US
utslib.citation.volume	47	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	0608 Zoology	en_US
utslib.for	0707 Veterinary Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access
pubs.issue	4	en_US
pubs.publication-status	Published	en_US
pubs.volume	47	en_US

Abstract:

© 2017 Australian Society for Parasitology Certain clinical isolates of Tritrichomonas foetus infect the urogenital tract of cattle while others infect the gastrointestinal tract of cats. Previous studies have identified subtle genetic differences between these isolates with the term “genotype” adopted to reflect host origin. The aim of this work was to seek evidence of host-specific adaptation and to clarify the relationship between T. foetus genotypes. To do this we characterised the proteomes of both genotypes using two-dimensional gel electrophoresis (2DE) coupled with LC-MS/MS. Our comparative analysis of the data revealed that both genotypes exhibited largely similar proteoform profiles; however differentiation was possible with 24 spots identified as having a four-fold or greater change. Deeper analysis using 2DE zymography and protease-specific fluorogenic substrates revealed marked differences in cysteine protease (CP) expression profiles between the two genotypes. These variances in CP activities could also account for the pathogenic and histopathological differences previously observed between T. foetus genotypes in cross-infection studies. Our findings highlight the importance of CPs as major determinants of parasite virulence and provide a foundation for future host-parasite interaction studies, with direct implications for the development of vaccines or drugs targeting T. foetus.

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http://hdl.handle.net/10453/109353